| |
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Las Brujas. |
|
Fecha : |
26/01/2024 |
|
Actualizado : |
26/01/2024 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
|
Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
|
Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
|
Complemento del título : |
Original full article. |
|
Fecha de publicación : |
2023 |
|
Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
|
ISSN : |
1983-0246 |
|
DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
|
Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
|
Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
|
Asunto categoría : |
L10 Genética y mejoramiento animal |
|
URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
|
|
Marc : |
LEADER 03124naa a2200361 a 4500
001 1064440
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a1983-0246
024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI
100 1 $aPICCOLI, R.J.
245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br --
520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.
653 $aDiagnosis
653 $aImmunohistochemistry
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPsittacosis
653 $aZoonosis
700 1 $aGRUCHOUSKEI, L.
700 1 $aBENINCA, A.L.V.
700 1 $aMAURENTE BERÓN, M.
700 1 $aCHENG, A.C.
700 1 $aDE ANDRADE, J.A.
700 1 $aFACCIN, M.
700 1 $aGRZEGOZEVSKI, A.P.
700 1 $aDA SILVA, G.C.R.
700 1 $aFRANÇA, J.C.
700 1 $aFERNANDES, N.L.M.
700 1 $aDE CARVALHO, A.L.
700 1 $aVIOTT, A.M.
773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Las Brujas (LB) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
| Registros recuperados : 687 | |
| 21. |  | DORSCH, M.; CASAUX, M.L.; CALLEROS, L.; ARÁOZ, V.; CAFFARENA, D.; MONESIGLIO, M.C.; BARCELLOS, M.; SILVEIRA, C.S.; PERDOMO, T.; BANCHERO, G.; UZAL, F.A.; FRAGA, M.; GIANNITTI, F. Corrigendum to "Placentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay". (Revista Argentina de Microbiologia (2022) 54(1) (25-30), (S0325754121000389), (10.1016/j.ram.2021.02.005)). Erratum. Revista Argentina de Microbiologia. 2023, Volume 55, Issue 4, Pages 397-398. https://doi.org/10.1016/j.ram.2023.11.002 -- OPEN ACCESS. Article history: Available online 21 December 2023; Version of Record 21 December 2023. -- Correspondence: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA),...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|    |
| 25. | | CASAUX, M.L.; NETO, W.S.; SCHILD, C.; COSTA, R.A. DA; MACÍAS-RIOSECO, M.; CAFFARENA, D.; SILVEIRA, C.S.; ARÁOZ, V.; DONCEL, B.; GIANNITTI, F.; FRAGA, M. Epidemiological and clinicopathological findings in 15 fatal outbreaks of salmonellosis in dairy calves and virulence genes in the causative Salmonella enterica Typhimurium and Dublin strains. Veterinary Microbiology - Research Paper. Brazilian Journal of Microbiology, 2023, volume 54, isuue 1, pp. 475-490. doi: https://doi.org/10.1007/s42770-022-00898-9 Article history: Received 06 December 2021; Accepted 20 December 2022; Published 05 January 2023. -- Correspondence author: Fraga, M.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 26. | | CIBILS-STEWART, X.; CALISTRO, P.; BARAIBAR, N.; GIAMBIASI, M.; GIANNITTI, F.; MACHADO, M.; SILVEIRA, C.S.; MENCHACA, A.; CAFFARENA, D.; SARAVIA, A. Escarabajo siete de oro (Astylus atromaculatus): actualización y recomendaciones en vísperas del verano 2024. Producción animal. Revista INIA Uruguay, Diciembre 2023, no.75 p.35-39. (Revista INIA; 75).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
|  |
| 28. | | BUSCHIAZZO, M.; MERINO, N.; DE MORI, J.; PASSADORE, A.; VILLARINO, A.; BERTOLA, B.; CONIBERTI, A.; FASIOLO, C. FPTA 353: Culmina una etapa y se evoluciona a una vitivinicultura cada vez más sostenible. Proyectos FPTA. Revista INIA Uruguay, Junio 2023, no.73, p.89-92. (Revista INIA; 73).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 30. | | VIETA, A.; ORIHUELA, C.; FASIOLO, C.; BAO, L.; LORENZO, E.; GALVÁN, G.; WATERSTON, S.; IURATO, A.; PRIMO, P.; MARTÍNEZ, F-; GODÍN, A.; VIANA, V. FPTA 383: Programa de integración de herramientas biológicas y culturales para el control de plagas y enfermedades en horticultura. Proyectos FPTA. Revista INIA Uruguay, Marzo 2023, no.72, p.112-114. (Revista INIA; 72).| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Las Brujas. |
| |
| 31. | | GIANNITTI, F.; GARCÍA, J. P.; ADAMS, V.; ARMENDANO, J.; BEINGESSER, J.; ROOD, J.; UZAL, F. A. Experimental acute Clostridium perfringens type D enterotoxemia in sheep is not characterized by specific renal lesions. Infectious Disease - Original Article. Veterinary Pathology. 2023, vol.60(4):412-419. https://doi.org/10.1177/03009858231171669 Article history: First published online May 12, 2023. -- Corresponding Author: Francisco A. Uzal, California Animal Health and Food Safety Laboratory, School of Veterinary Medicine, University of California at Davis, 105 W CVentral Ave,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
| |
| 34. | | RABAZA, A.; FRAGA, M.; MENDOZA, A.; GIANNITTI, F. A meta-analysis of the effects of colostrum heat-treatment on its viscosity and IgG concentration, and the transfer of passive immunity in newborn dairy calves. Journal of Dairy Science, 2023. https://doi.org/10.3168/jds.2022-22555 -- [Article In Press]. OPEN ACCESS. Article history: Received July 20, 2022; Accepted April 19, 2023; Published online May 08, 2023. -- Corresponding authors: Ana Rabaza, arabaza@pasteur.edu.uy; Federico Giannitti, fgiannitti@inia.org.uy -- License: This is an open access...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
| |
| 39. | | MACHADO, M.; OLIVEIRA, L.G.S.; SCHILD, C.; BOABAID, F.; LUCAS, M.; BURONI, F.; CASTRO, M. B.; RIET-CORREA, F. Lantana camara poisoning in cattle that took refuge during a storm in a forest invaded by this plant. Toxicon, 2023, Volume 229, article 107124. https://doi.org/10.1016/j.toxicon.2023.107124 Article history: Received 8 March 2023; Received in revised form 7 April 2023; Accepted 10 April 2023; Available online 11 April 2023. -- Corresponding author: Riet-Correa, F.; Instituto Nacional de Investigación Agropecuaria,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
| |
| Registros recuperados : 687 | |
|
| Expresión de búsqueda válido. Check! |
|
|
