03124naa a2200361 a 450000100080000000500110000800800410001902200140006002400480007410000180012224501730014026000090031350003030032252016230062565300140224865300250226265300560228765300160234365300130235970000200237270000200239270000240241270000160243670000210245270000150247370000230248870000210251170000180253270000220255070000220257270000160259477301520261010644402024-01-26       2023    bl uuuu     u00u1 u    #d  a1983-02467 a10.0.94.6/bjvp.1983-0246.v16i3p159-1662DOI1 aPICCOLI, R.J.  aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.h[electronic resource]  c2023  aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br  --  aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.  aDiagnosis  aImmunohistochemistry  aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA  aPsittacosis  aZoonosis1 aGRUCHOUSKEI, L.1 aBENINCA, A.L.V.1 aMAURENTE BERÓN, M.1 aCHENG, A.C.1 aDE ANDRADE, J.A.1 aFACCIN, M.1 aGRZEGOZEVSKI, A.P.1 aDA SILVA, G.C.R.1 aFRANÇA, J.C.1 aFERNANDES, N.L.M.1 aDE CARVALHO, A.L.1 aVIOTT, A.M.  tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166  -- OPEN ACCESS.