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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
10/03/2021 |
Actualizado : |
14/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
NASCIMENTO, D. C.; DINI, M.; CARPENEDO, S.; RASEIRA, M. DO C. B. |
Afiliación : |
D. C. NASCIMENTO, Postgraduate Program in Agronomy (PPGA), Faculty of Agronomy "Eliseu Maciel", Federal University of Pelotas (FAEM-UFPel), Pelotas (RS), Brazil; MAXIMILIANO ANTONIO DINI VIÑOLY, Postgraduate Program in Agronomy (PPGA), Faculty of Agronomy "Eliseu Maciel", Federal University of Pelotas (FAEM-UFPel), Pelotas (RS), Brazil; S. CARPENEDO, Laboratory of Fruit Breeding, Embrapa Temperate Agriculture Pelotas (RS), Brazil; M. C. B. RASEIRA, Laboratory of Fruit Breeding, Embrapa Temperate Agriculture Pelotas (RS), Brazil. |
Título : |
Germination and development of 'Precocinho' peach embryos: asepsis and use of PPM tm in culture medium. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Journal of Experimental Agriculture International, 2018, v. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065 |
ISSN : |
2457-0591 |
DOI : |
10.9734/JEAI/2018/41065 |
Idioma : |
Inglés |
Notas : |
Article history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018.
Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Republic of Serbia.
Reviewers: (1) Zeynel Dalkiliç, College of Agriculture, Adnan Menderes University, Turkey. (2) Nebi Bilir, Suleyman Demirel University, Turkey. (3) Martin Potgieter, University of Limpopo, South Africa. Complete Peer review History: http://www.sciencedomain.org/review-history/24416 |
Contenido : |
ABSTRACT.
Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos. |
Palabras claves : |
Disinfestation; Embryo culture; Immature embryos. |
Thesagro : |
PRUNUS PERSICA (L.). |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15317/1/Nascimento-et-al.-2018.-Germination-and-Development-of-Precocinho-Peach-JEAI.pdf
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Marc : |
LEADER 02746naa a2200241 a 4500 001 1061812 005 2021-06-14 008 2018 bl uuuu u00u1 u #d 022 $a2457-0591 024 7 $a10.9734/JEAI/2018/41065$2DOI 100 1 $aNASCIMENTO, D. C. 245 $aGermination and development of 'Precocinho' peach embryos$basepsis and use of PPM tm in culture medium.$h[electronic resource] 260 $c2018 500 $aArticle history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018. Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Republic of Serbia. Reviewers: (1) Zeynel Dalkiliç, College of Agriculture, Adnan Menderes University, Turkey. (2) Nebi Bilir, Suleyman Demirel University, Turkey. (3) Martin Potgieter, University of Limpopo, South Africa. Complete Peer review History: http://www.sciencedomain.org/review-history/24416 520 $aABSTRACT. Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos. 650 $aPRUNUS PERSICA (L.) 653 $aDisinfestation 653 $aEmbryo culture 653 $aImmature embryos 700 1 $aDINI, M. 700 1 $aCARPENEDO, S. 700 1 $aRASEIRA, M. DO C. B. 773 $tJournal of Experimental Agriculture International, 2018$gv. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
Fecha actual : |
21/02/2014 |
Actualizado : |
14/10/2019 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
RIOS, A.; OLIVA, M.A.; LOPEZ, N. F. |
Afiliación : |
AMALIA RIOS GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Avaliaçao do crescimento de plantas de três espécies cultivadas em solo proveniente de três sistemas de rotaçao no Uruguai |
Fecha de publicación : |
1995 |
Fuente / Imprenta : |
In: ASOCIACIÓN LATINOAMERICANA DE MALEZAS. CONGRESO LATINOAMERICANO DE MALEZAS, 12. 21-23 MARZO 1995, MONTEVIDEO (URY). RÍOS, A.; FERNÁNDEZ, G. (EDS.). Conferencias y trabajos. Montevideo: INIA, 1995. |
Páginas : |
p. 247-257 |
Serie : |
(INIA Serie Técnica ; 56) |
Idioma : |
Español |
Notas : |
Parte de tesis de Doctorado del primer autor. Congreso declarado de interés nacional (decreto del Poder Ejecutivo octubre/1994) |
Thesagro : |
COMPETICION VEGETAL; LOLIUM MULTIFLORUM; RAPHANUS; ROTACION DE CULTIVOS; SISTEMAS DE CULTIVO; TRIGO; TRITICUM AESTIVUM; URUGUAY. |
Asunto categoría : |
-- A50 Investigación agraria |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8417/1/st-56A-1995-p.247-257.pdf
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Marc : |
LEADER 01053naa a2200265 a 4500 001 1012180 005 2019-10-14 008 1995 bl uuuu u00u1 u #d 100 1 $aRIOS, A. 245 $aAvaliaçao do crescimento de plantas de três espécies cultivadas em solo proveniente de três sistemas de rotaçao no Uruguai 260 $c1995 300 $ap. 247-257 490 $a(INIA Serie Técnica ; 56) 500 $aParte de tesis de Doctorado del primer autor. Congreso declarado de interés nacional (decreto del Poder Ejecutivo octubre/1994) 650 $aCOMPETICION VEGETAL 650 $aLOLIUM MULTIFLORUM 650 $aRAPHANUS 650 $aROTACION DE CULTIVOS 650 $aSISTEMAS DE CULTIVO 650 $aTRIGO 650 $aTRITICUM AESTIVUM 650 $aURUGUAY 700 1 $aOLIVA, M.A. 700 1 $aLOPEZ, N. F. 773 $tIn: ASOCIACIÓN LATINOAMERICANA DE MALEZAS. CONGRESO LATINOAMERICANO DE MALEZAS, 12. 21-23 MARZO 1995, MONTEVIDEO (URY). RÍOS, A.; FERNÁNDEZ, G. (EDS.). Conferencias y trabajos. Montevideo: INIA, 1995.
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