02746naa a2200241 a 450000100080000000500110000800800410001902200140006002400330007410000220010724501310012926000090026050005520026952013940082165000240221565300190223965300190225865300210227770000130229870000180231170000250232977301500235410618122021-06-14       2018    bl uuuu     u00u1 u    #d  a2457-05917 a10.9734/JEAI/2018/410652DOI1 aNASCIMENTO, D. C.  aGermination and development of 'Precocinho' peach embryosbasepsis and use of PPM tm in culture medium.h[electronic resource]  c2018  aArticle history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018. Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Republic of Serbia. Reviewers: (1) Zeynel Dalkiliç, College of Agriculture, Adnan Menderes University, Turkey. (2) Nebi Bilir, Suleyman Demirel University, Turkey. (3) Martin Potgieter, University of Limpopo, South Africa. Complete Peer review History: http://www.sciencedomain.org/review-history/24416  aABSTRACT. Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The  experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.  aPRUNUS PERSICA (L.)  aDisinfestation  aEmbryo culture  aImmature embryos1 aDINI, M.1 aCARPENEDO, S.1 aRASEIRA, M. DO C. B.  tJournal of Experimental Agriculture International, 2018gv. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065