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Biblioteca (s) : |
INIA Tacuarembó. |
Fecha : |
18/10/2018 |
Actualizado : |
18/10/2018 |
Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
Autor : |
MARTÍNEZ, G.; SCOZ, R. |
Afiliación : |
GONZALO ANIBAL MARTINEZ CROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ROBERTO JAVIER SCOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Control biológico de la chiche del eucalipto Thaumastocoris peregrinus en Uruguay: principales avances. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: INTA. ASOCIACIÓN DE INGENIEROS AGRÓNOMOS DEL NORDESTE DE ENTRE RÍOS (AIANER). Jornadas forestales de Entre Ríos, 32., Concordia 2018. Concordia: INTA; AIANER, 2018. |
Páginas : |
p. 13-21 |
Idioma : |
Español |
Contenido : |
El control biológico es la herramienta principal para el manejo de plagas forestales. Para iniciar el control biológico de la chinche del eucalipto en Uruguay se importaron hue\l)s parasitados por Cleruchoides noackae desde una cria masiva en Brasil y se inició una colonia de cria. Las avispas fueron criadas en tubos plásticos con hue\l)s del hospedero y una solución de agua y miel. Se comparó un conjunto de parámetros de calidad contra estándares establecidos a priori. Se evaluó el efecto de la temperatura sobre el tiempo de desarrollo. A partir de marzo de 2013 se real izaron liberaciones anuales de parasitoides en rodales de Eucalyplus durante los veranos. Las tasas de emergencia y de supervivencia fueron estables o se incrementaron a lo largo de 30 generaciones. La razón sexual mostró una ligera desviación hacia las hembras. El ciclo se enlenteció a 18°C con respecto a la cria a 22°C sin afectar la productividad. Se lograron instalar tres poblaciones silvestres de avispas en sitios de liberación. Relevamientos a campo revelaron que las avispas sobrevivieron por dos años. Los parasitoides colectados a campo presentaron mejores indices de calidad que los de la cria de laboratorio. |
Palabras claves : |
CALIDAD DE CRÍA; CLERUCHOIDES NOACKAE; LIBERACCIÓN INUNDATIVA. |
Thesagro : |
CONTROL BIOLÓGICO; EUCALYPTUS; FORESTACIÓN; THAUMASTOCORIS PEREGRINUS; URUGUAY. |
Asunto categoría : |
H10 Plagas de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11611/1/Jornadas-forestales-de-Entre-Rios-2018.pdf
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Marc : |
LEADER 02014nam a2200229 a 4500 001 1059218 005 2018-10-18 008 2018 bl uuuu u01u1 u #d 100 1 $aMARTÍNEZ, G. 245 $aControl biológico de la chiche del eucalipto Thaumastocoris peregrinus en Uruguay$bprincipales avances. 260 $aIn: INTA. ASOCIACIÓN DE INGENIEROS AGRÓNOMOS DEL NORDESTE DE ENTRE RÍOS (AIANER). Jornadas forestales de Entre Ríos, 32., Concordia 2018. Concordia: INTA; AIANER$c2018 300 $ap. 13-21 520 $aEl control biológico es la herramienta principal para el manejo de plagas forestales. Para iniciar el control biológico de la chinche del eucalipto en Uruguay se importaron hue\l)s parasitados por Cleruchoides noackae desde una cria masiva en Brasil y se inició una colonia de cria. Las avispas fueron criadas en tubos plásticos con hue\l)s del hospedero y una solución de agua y miel. Se comparó un conjunto de parámetros de calidad contra estándares establecidos a priori. Se evaluó el efecto de la temperatura sobre el tiempo de desarrollo. A partir de marzo de 2013 se real izaron liberaciones anuales de parasitoides en rodales de Eucalyplus durante los veranos. Las tasas de emergencia y de supervivencia fueron estables o se incrementaron a lo largo de 30 generaciones. La razón sexual mostró una ligera desviación hacia las hembras. El ciclo se enlenteció a 18°C con respecto a la cria a 22°C sin afectar la productividad. Se lograron instalar tres poblaciones silvestres de avispas en sitios de liberación. Relevamientos a campo revelaron que las avispas sobrevivieron por dos años. Los parasitoides colectados a campo presentaron mejores indices de calidad que los de la cria de laboratorio. 650 $aCONTROL BIOLÓGICO 650 $aEUCALYPTUS 650 $aFORESTACIÓN 650 $aTHAUMASTOCORIS PEREGRINUS 650 $aURUGUAY 653 $aCALIDAD DE CRÍA 653 $aCLERUCHOIDES NOACKAE 653 $aLIBERACCIÓN INUNDATIVA 700 1 $aSCOZ, R.
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INIA Tacuarembó (TBO) |
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
26/01/2024 |
Actualizado : |
26/01/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
Complemento del título : |
Original full article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
ISSN : |
1983-0246 |
DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500 001 1064440 005 2024-01-26 008 2023 bl uuuu u00u1 u #d 022 $a1983-0246 024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI 100 1 $aPICCOLI, R.J. 245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- 520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. 653 $aDiagnosis 653 $aImmunohistochemistry 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aPsittacosis 653 $aZoonosis 700 1 $aGRUCHOUSKEI, L. 700 1 $aBENINCA, A.L.V. 700 1 $aMAURENTE BERÓN, M. 700 1 $aCHENG, A.C. 700 1 $aDE ANDRADE, J.A. 700 1 $aFACCIN, M. 700 1 $aGRZEGOZEVSKI, A.P. 700 1 $aDA SILVA, G.C.R. 700 1 $aFRANÇA, J.C. 700 1 $aFERNANDES, N.L.M. 700 1 $aDE CARVALHO, A.L. 700 1 $aVIOTT, A.M. 773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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