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| Registros recuperados : 594 | |
| 81. |  | CONSORCIO CITRÍCOLA DEL URUGUAY Catálogo de variedades cítricas. Salto (UY): INIA-MGAP-UPEFRUY, 2020. 19 p. El denominado Consorcio Citrícola del Uruguay está integrado por INIA Salto Grande (con sede en la zona de Salto Grande), conjuntamente con el Ministerio de Ganadería Agricultura y Pesca (MGAP) y la Unión de Productores de Frutas del...| Biblioteca(s): INIA Las Brujas. |
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| 86. | | ARRUABARRENA, A.; PINTOS, P.; LUQUE, E.; MOLTINI, A.; RIVAS, F.; LADO, J. Characterization of carotenoid accumulation in different hybrids from the Uruguayan Citrus Breeding Program. Conference Paper. Acta Horticulturae, 15 January 2019, N° 1230, p. 67-72. (Acta Horticulturae; 1230) Article history: Published 15 January 2019.
In: Acta Horticulturae (ISHS) 1230: IV International Symposium on Citrus Biotechnology. Editors: F. Rivas, Y. Erner, F.G. Gmitter Jr. Place: Canelones, Uruguay.| Biblioteca(s): INIA Las Brujas. |
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| 90. | | Citricos : evolución reciente para la exportación ln: Asociación de Ingenieros Agronomos del Uruguay. Anuario de divulgación técnica. Montevideo (Uruguay): Asociación de Ingenieros Agronomos del Uruguay, 1983. p126-129| Biblioteca(s): INIA La Estanzuela. |
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| 95. | | AGUSTÍ, M Citricultura. 2a. ed. rev. y ampl. Madrid: Mundi Prensa, 2003. 422 p.| Biblioteca(s): INIA Las Brujas; INIA Salto Grande. |
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| 99. | | Francis, M.; Verdier, R.; Etchevers, A.; Peralta, A.M. Citrus canker diagnostic using PCR: perspectives of application in Uruguay ln: Encuentro Latinoamericano de Biotecnología Vegetal, 2.; Encuentro Argentino de Biotecnología Vegetal, 3., 1995, Puerto Iguazú, Argentina REDBIO 95. Puerto Iguazú (Argentina): REDBIO, 1995. p. G.3| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 594 | |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
27/04/2021 |
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Actualizado : |
27/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SIMEONE, M.; GÓMEZ, C.; BERTALMIO, A.; RUIZ, E.; HAUTEVILLE, C.; GODOY, L.; TITO, B.; GARCÍA, M.L. |
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Afiliación : |
MELINA SIMEONE, Instituto de Biotecnología y Biología Molecular, CCT-La Plata CONICET-UNLP Facultad de Ciencias Exactas, UNLP, La Plata, Argentina; CLAUDIO GÓMEZ, Estación Experimental Agropecuaria Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA), Entre Ríos, Argentina; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ESPERANZA RUIZ, Laboratorio de Investigación y Desarrollo de Bioactivos, Facultad de Ciencias Exactas, UNLP, La Plata, Argentina; CLAUDIA HAUTEVILLE, Estación Experimental Agropecuaria Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA), Entre Ríos, Argentina; LAURA GODOY SUÁREZ, Estación Experimental Agropecuaria Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA), Entre Ríos, Argentina; BLAS TITO, Estación Experimental Agropecuaria Concordia, Instituto Nacional de Tecnología Agropecuaria (INTA), Entre Ríos, Argentina; MARÍA L. GARCÍA, Instituto de Biotecnología y Biología Molecular, CCT?La Plata CONICET?UNLP Facultad de Ciencias Exactas, UNLP, La Plata, Argentina. |
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Título : |
Detection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Plant Pathology, May 2021, Volume 70, Issue 4, Pages 980-986. Doi: https://doi.org/10.1111/ppa.13341 |
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ISSN : |
0032-0862 |
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DOI : |
10.1111/ppa.13341 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received, 8 September 2020; Accepted, 28 December 2020, First published, 18 January 2021.
This work was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT) PICT 2014‐1007 and PICT Start UP 2014‐3762, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Proyectos de Investigación de Unidades Ejecutoras?(IBBM), Universidad Nacional de La Plata (UNLP) X‐692, and Instituto Nacional de Tecnología Agropecuaria (INTA) (PNFRU‐1172; 11721; ERIOS‐630081, PD I081 and RIST I091). M.S. was supported by ANPCyT and CONICET. M.L.G. belongs to CONICET and Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, UNLP. We thank Beatriz Stein and Julia Figueroa from the Estación Experimental Agroindustrial Obispo Colombres (EEOC), Tucumán, for providing samples from their collection and Magalí Gabrielli for technical assistance in the total RNA extractions. We thank Pedro Moreno for helpful discussion and critical reading of the manuscript. |
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Contenido : |
ABSTRACT.
Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees.
© 2021 British Society for Plant Pathology |
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Palabras claves : |
Citrus psorosis virus; Diagnostic parameters; RT-qPCR. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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Marc : |
LEADER 03265naa a2200277 a 4500
001 1061995
005 2021-04-27
008 2021 bl uuuu u00u1 u #d
022 $a0032-0862
024 7 $a10.1111/ppa.13341$2DOI
100 1 $aSIMEONE, M.
245 $aDetection of citrus psorosis virus by RT‐qPCR validated by diagnostic parameters.$h[electronic resource]
260 $c2021
500 $aArticle history: Received, 8 September 2020; Accepted, 28 December 2020, First published, 18 January 2021. This work was supported by Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT) PICT 2014‐1007 and PICT Start UP 2014‐3762, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Proyectos de Investigación de Unidades Ejecutoras?(IBBM), Universidad Nacional de La Plata (UNLP) X‐692, and Instituto Nacional de Tecnología Agropecuaria (INTA) (PNFRU‐1172; 11721; ERIOS‐630081, PD I081 and RIST I091). M.S. was supported by ANPCyT and CONICET. M.L.G. belongs to CONICET and Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, UNLP. We thank Beatriz Stein and Julia Figueroa from the Estación Experimental Agroindustrial Obispo Colombres (EEOC), Tucumán, for providing samples from their collection and Magalí Gabrielli for technical assistance in the total RNA extractions. We thank Pedro Moreno for helpful discussion and critical reading of the manuscript.
520 $aABSTRACT. Citrus psorosis virus (CPsV) is the causal agent of psorosis, an important disease of citrus. Sanitary and certification programmes helped reduce disease damage caused by psorosis and other graft‐transmissible diseases in many citrus‐growing regions. For quarantine and certification programmes, most of these diseases are currently diagnosed using biological indexing (BI) on sensitive indicator plants. In the case of citrus psorosis, CPsV can be detected by molecular methods such as quantitative reverse transcription PCR (RT‐qPCR), which is cheaper and faster than BI, but sensitivity, reliability, and reproducibility of both procedures have not been compared so far. In this work, 128 plants from Argentina and Uruguay were analysed using BI and CPsV detection by the RT‐qPCR assay. Almost perfect agreement between both diagnostic procedures and sensitivity, specificity, and estimated likelihood ratios indicate that RT‐qPCR is equivalent to BI for citrus psorosis diagnosis, thus providing confidence in the quick diagnostic procedure to monitor the sanitary status of citrus trees. © 2021 British Society for Plant Pathology
653 $aCitrus psorosis virus
653 $aDiagnostic parameters
653 $aRT-qPCR
700 1 $aGÓMEZ, C.
700 1 $aBERTALMIO, A.
700 1 $aRUIZ, E.
700 1 $aHAUTEVILLE, C.
700 1 $aGODOY, L.
700 1 $aTITO, B.
700 1 $aGARCÍA, M.L.
773 $tPlant Pathology, May 2021, Volume 70, Issue 4, Pages 980-986. Doi: https://doi.org/10.1111/ppa.13341
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