|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
05/05/2017 |
Actualizado : |
24/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
OLINDA, R. G.; MAIA, L. A.; CARGNELUTTI, J. F.; GOIS, R. C. S.; BATISTA, J. S.; DANTAS, A. F. M.; FLORES, E. F.; RIET-CORREA, F. |
Afiliación : |
FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Programa de Pós-Graduação, em Medicina Veterinária, Hospital Veterinário, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande (UFCG), Brasil. |
Título : |
Swinepox dermatitis in backyard pigs in Northeastern Brazil. |
Fecha de publicación : |
2016 |
Fuente / Imprenta : |
Pesquisa Veterinária Brasileira, Brasília, DF v. 36, n. 6, p. 468-472, 2016. |
DOI : |
10.1590/S0100-736X2016000600002 |
Idioma : |
Inglés |
Notas : |
Article History:Received on June 11, 2015.//Accepted for publication on March 23, 2016. OPEN ACCESS |
Contenido : |
This article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs. MenosThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvi... Presentar Todo |
Palabras claves : |
Doença viral. |
Thesagro : |
Dermatite; Dermatitis; Poxviridae; Suíno; Swine; Varíola; Viral diseases of animals and humans. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/6764/1/Pesq.-Vet.-Bras.-366p.468-472-2016.pdf
|
Marc : |
LEADER 02654naa a2200325 a 4500 001 1057176 005 2021-06-24 008 2016 bl uuuu u00u1 u #d 024 7 $a10.1590/S0100-736X2016000600002$2DOI 100 1 $aOLINDA, R. G. 245 $aSwinepox dermatitis in backyard pigs in Northeastern Brazil.$h[electronic resource] 260 $c2016 500 $aArticle History:Received on June 11, 2015.//Accepted for publication on March 23, 2016. OPEN ACCESS 520 $aThis article describes five outbreaks of swinepox in backyard pigs in Northeastern Brazil. It affected backyard pigs from herds of poor hygienic-sanitary conditions with severe fly and lice infestations. The morbidity ranged from 33.3 to 100% among affected herds, with mortality reaching up to 60%. The affected pigs developed multifocal to coalescent gray to white papules and blisters in the skin, with eventual eruptions, evolving to erosions and crusts. In addition to skin lesions, affected piglets presented apathy, anorexia and fever. The disease was auto-limiting, resolving within 15 to 25 days. Histological examination revealed proliferative and ulcerative vesiculopustular dermatitis with ballooning degeneration of epithelial cells, perivascular inflammatory infiltrates of lymphocytes, plasma cells, neutrophils, eosinophils and some macrophages in the dermis. Intracytoplasmic eosinophilic inclusions were consistently observed in keratinocytes. Total DNA extracted from fresh tissue fragments obtained from one outbreak and formalin-fixed, paraffin-embedded (FFPE) tissue from the other four outbreaks was submitted to polymerase chain reaction (PCR) for Swinepox virus (SWPV) and Vaccinia virus (VACV). Genetic SWPV material was identified by PCR in fresh material from one outbreak. Nucleotide sequencing and phylogenetic analysis of the PCR amplicons (viral polymerase gene) demonstrated 100% homology with sequences from SWPV. All tissues were PCR negative for VACV. Swine poxvirus is present in backyard pigs in Northeastern Brazil, indicating the need of including SWPV in the differential diagnosis of dermatitis in pigs. 650 $aDermatite 650 $aDermatitis 650 $aPoxviridae 650 $aSuíno 650 $aSwine 650 $aVaríola 650 $aViral diseases of animals and humans 653 $aDoença viral 700 1 $aMAIA, L. A. 700 1 $aCARGNELUTTI, J. F. 700 1 $aGOIS, R. C. S. 700 1 $aBATISTA, J. S. 700 1 $aDANTAS, A. F. M. 700 1 $aFLORES, E. F. 700 1 $aRIET-CORREA, F. 773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 36, n. 6, p. 468-472, 2016.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
| Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
04/01/2021 |
Actualizado : |
04/01/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
-- - -- |
Autor : |
CRUPPE, G.; SILVA, P.; SILVA, C. LEMES DA; PETERSON, G.; PEDLEY, K. F.; CRUZ, C. D.; ASIF, M.; LOLLATO, R. P.; FRITZ, A. K.; VALENT, B. |
Afiliación : |
GIOVANA CRUPPE, Department of Plant pathology, Kansas State University, USA.; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. Deparment of Plant Pathology, Kansas State University, KS, USA.; CRISTIANO LEMES DA SILVA, Corteva Agriscience, Windfall, IN, USA.; GARY PETERSON, United State Department of Agriculture Agricultural Research Service, Foreign Desease-Weed Science Research Unit, USA.; KERRY F. PEDLEY, United State Department of Agriculture Agricultural Research Service, Foreign Desease-Weed Science Research Unit, USA.; CHRISTIAN D. CRUZ, Department of Botany and Plant Pathology, Purdue University, USA.; MOHAMMAD ASIF, Department of Agronomy, Kansas State University, USA. Heartland Plant Innovations, Manhattan KS, USA.; ROMULO P. LOLLATO, Department of Agronomy, Kansas State University, USA.; ALLAN K. FRITZ, Department of Agronomy, Kansas State University, USA.; BARBARA VALENT, Deparment of Plant Pathology, Kansas State University, USA. |
Título : |
Genome wide association reveals limited benefits of pyramiding the 1B and 1D loci with the 2Nv S translocation for wheat blast control. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
Crop Science [First Online]. DOI: https://doi.org/10.1002/csc2.20397 |
Páginas : |
42 p. |
DOI : |
10.1002/csc2.20397 |
Idioma : |
Inglés |
Notas : |
Article history: Manuscript received, 09 June 2020 // Manuscript accepted, 23 October 2020 // Accepted manuscript online, 31 October 2020 // Version of Record online,
22 December 2020.
Corresponding authors: Barbara Valent, bvalent@ksu.edu ; Giovana Cruppe, gicruppe@ksu.edu |
Contenido : |
Resistance to wheat spike blast (WSB), caused by the Magnaporthe oryzae triticum pathotype (MoT), has relied upon a single major source: the 2NvS translocation introgressed from the wild relative Aegilops ventricosa Tausch. However, this resistance is partial and recently partially overcome by newer MoT races. To characterize potential novel loci conferring resistance to WSB, we conducted a genome?wide association study (GWAS) using a diverse panel of 384 wheat genotypes phenotyped under three controlled?environment conditions using MoT isolates T?25 (301 genotypes), B?71 (87 genotypes), and 008 (49 genotypes). Genotyping?by?sequencing identified 13,175 single nucleotide polymorphisms (SNPs) after filtering. Principal components analysis (PCA) identified two clusters based on the presence or absence of the 2NvS translocation, and the first three PCAs explained 13% of the genetic variation. Three individual analyses were performed (full [all genotypes combined], 2NvS genotypes only, and non?2NvS genotypes only) using a linear mixed model and a threshold of significance of false discovery rate at 5%. Association analysis detected 25 significant SNPs for the full GWAS with isolate T?25, in which 21 were mapped on chromosome 2A in the same physical position as the 2NvS translocation. Highly significant linkage disequilibrium among these SNPs suggested they might tag the same quantitative trait locus (QTL). No significant associations were identified with isolates B?71 and 008, likely due to the small sample size. A QTL pyramiding analysis showed that the combination of multiple QTL was not statistically different from the individual effect of the 2A QTL. Further validation of these genomic regions can aid breeding for broad spectrum and durable WSB resistanc MenosResistance to wheat spike blast (WSB), caused by the Magnaporthe oryzae triticum pathotype (MoT), has relied upon a single major source: the 2NvS translocation introgressed from the wild relative Aegilops ventricosa Tausch. However, this resistance is partial and recently partially overcome by newer MoT races. To characterize potential novel loci conferring resistance to WSB, we conducted a genome?wide association study (GWAS) using a diverse panel of 384 wheat genotypes phenotyped under three controlled?environment conditions using MoT isolates T?25 (301 genotypes), B?71 (87 genotypes), and 008 (49 genotypes). Genotyping?by?sequencing identified 13,175 single nucleotide polymorphisms (SNPs) after filtering. Principal components analysis (PCA) identified two clusters based on the presence or absence of the 2NvS translocation, and the first three PCAs explained 13% of the genetic variation. Three individual analyses were performed (full [all genotypes combined], 2NvS genotypes only, and non?2NvS genotypes only) using a linear mixed model and a threshold of significance of false discovery rate at 5%. Association analysis detected 25 significant SNPs for the full GWAS with isolate T?25, in which 21 were mapped on chromosome 2A in the same physical position as the 2NvS translocation. Highly significant linkage disequilibrium among these SNPs suggested they might tag the same quantitative trait locus (QTL). No significant associations were identified with isolates B?71 and 008, l... Presentar Todo |
Palabras claves : |
2Nv S TRANSLOCATION; DURABLE RESISTANCE; MAGNAPORTHE ORYZAE TRITICUM; TRIGO; WHEAT; WHEAT SPIKE BLAST. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 03037naa a2200337 a 4500 001 1061645 005 2021-01-04 008 2020 bl uuuu u00u1 u #d 024 7 $a10.1002/csc2.20397$2DOI 100 1 $aCRUPPE, G. 245 $aGenome wide association reveals limited benefits of pyramiding the 1B and 1D loci with the 2Nv S translocation for wheat blast control.$h[electronic resource] 260 $c2020 300 $a42 p. 500 $aArticle history: Manuscript received, 09 June 2020 // Manuscript accepted, 23 October 2020 // Accepted manuscript online, 31 October 2020 // Version of Record online, 22 December 2020. Corresponding authors: Barbara Valent, bvalent@ksu.edu ; Giovana Cruppe, gicruppe@ksu.edu 520 $aResistance to wheat spike blast (WSB), caused by the Magnaporthe oryzae triticum pathotype (MoT), has relied upon a single major source: the 2NvS translocation introgressed from the wild relative Aegilops ventricosa Tausch. However, this resistance is partial and recently partially overcome by newer MoT races. To characterize potential novel loci conferring resistance to WSB, we conducted a genome?wide association study (GWAS) using a diverse panel of 384 wheat genotypes phenotyped under three controlled?environment conditions using MoT isolates T?25 (301 genotypes), B?71 (87 genotypes), and 008 (49 genotypes). Genotyping?by?sequencing identified 13,175 single nucleotide polymorphisms (SNPs) after filtering. Principal components analysis (PCA) identified two clusters based on the presence or absence of the 2NvS translocation, and the first three PCAs explained 13% of the genetic variation. Three individual analyses were performed (full [all genotypes combined], 2NvS genotypes only, and non?2NvS genotypes only) using a linear mixed model and a threshold of significance of false discovery rate at 5%. Association analysis detected 25 significant SNPs for the full GWAS with isolate T?25, in which 21 were mapped on chromosome 2A in the same physical position as the 2NvS translocation. Highly significant linkage disequilibrium among these SNPs suggested they might tag the same quantitative trait locus (QTL). No significant associations were identified with isolates B?71 and 008, likely due to the small sample size. A QTL pyramiding analysis showed that the combination of multiple QTL was not statistically different from the individual effect of the 2A QTL. Further validation of these genomic regions can aid breeding for broad spectrum and durable WSB resistanc 653 $a2Nv S TRANSLOCATION 653 $aDURABLE RESISTANCE 653 $aMAGNAPORTHE ORYZAE TRITICUM 653 $aTRIGO 653 $aWHEAT 653 $aWHEAT SPIKE BLAST 700 1 $aSILVA, P. 700 1 $aSILVA, C. LEMES DA 700 1 $aPETERSON, G. 700 1 $aPEDLEY, K. F. 700 1 $aCRUZ, C. D. 700 1 $aASIF, M. 700 1 $aLOLLATO, R. P. 700 1 $aFRITZ, A. K. 700 1 $aVALENT, B. 773 $tCrop Science [First Online]. DOI: https://doi.org/10.1002/csc2.20397
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Treinta y Tres (TT) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|