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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
12/09/2014 |
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Actualizado : |
08/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
CARLOMAGNO, M.; MATHO, C.; CANTOU, G.; SANBORN, J.R.; LAST, J.A.; HAMMOCK, B.D.; ROEL, A.; GONZÁLEZ, D.; GONZÁLEZ-SAPIENZA, G. |
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Afiliación : |
MARIA GUILLERMINA CANTOU MAYOL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture. |
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Fecha de publicación : |
2010 |
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Fuente / Imprenta : |
Journal of Agricultural and Food Chemistry, 2010, v. 58, no.7, p. 4367-4371. |
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ISSN : |
0021-8561 |
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DOI : |
10.1021/jf9043259 |
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Idioma : |
Inglés |
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Notas : |
Article history: received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010. |
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Contenido : |
Abstract:
The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can
contribute to minimizing the release of the herbicide in the environment. |
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Palabras claves : |
ELISA; HPLC; RICE. |
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Thesagro : |
ARROZ; CLOMAZONE; CONTROL DE MALEZAS; ESCARDA; HERBICIDAS; ORYZA SATIVA. |
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Asunto categoría : |
H60 Malezas y escardas |
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Marc : |
LEADER 02181naa a2200361 a 4500
001 1050191
005 2019-10-08
008 2010 bl uuuu u00u1 u #d
022 $a0021-8561
024 7 $a10.1021/jf9043259$2DOI
100 1 $aCARLOMAGNO, M.
245 $aA clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture.$h[electronic resource]
260 $c2010
500 $aArticle history: received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010.
520 $aAbstract: The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment.
650 $aARROZ
650 $aCLOMAZONE
650 $aCONTROL DE MALEZAS
650 $aESCARDA
650 $aHERBICIDAS
650 $aORYZA SATIVA
653 $aELISA
653 $aHPLC
653 $aRICE
700 1 $aMATHO, C.
700 1 $aCANTOU, G.
700 1 $aSANBORN, J.R.
700 1 $aLAST, J.A.
700 1 $aHAMMOCK, B.D.
700 1 $aROEL, A.
700 1 $aGONZÁLEZ, D.
700 1 $aGONZÁLEZ-SAPIENZA, G.
773 $tJournal of Agricultural and Food Chemistry, 2010$gv. 58, no.7, p. 4367-4371.
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
15/04/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASANOVAS-MASSANA, A.; HAMOND, C.; SANTOS, L.A.; DE OLIVEIRA, D.; HACKER, K.P.; BALASSIANO, I.; COSTA, F.; MEDEIROS, M.A.; REIS, M.G.; KO, A.I.; WUNDER, E.A. |
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Afiliación : |
ARNAU CASANOVAS-MASSANA, Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA.; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; INIA (UMPI), Institut Pasteur de Montevideo; Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA.; LUCIANE A. SANTOS, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; DAIANA DE OLIVEIRA, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; KATHRYN P. HACKER, Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA; Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; ILANA BALASSIANO, Centro de Refernencia Nacional para Leptospirose/Coleçao de Leptospira, Laboratório de Zoonoses Bacterianas, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.; FEDERICO COSTA,, Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA; Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; MARCO A. MEDEIROS, Bio-Manguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, Brazil.; MITERMAYER G. REIS,, Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; ALBERT I. KO, Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA; Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil.; ELSIO A. WUNDER, Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA; Moniz Institute, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Bahia, Brazil. |
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Título : |
Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
International Journal of Systematic and Evolutionary Microbiology, 1 March 2020, Volume 70, Issue 3, Pages 1450-1456. OPEN ACCESS. Doi: https://doi.org/10.1099/ijsem.0.003480 |
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DOI : |
10.1099/ijsem.0.003480 |
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Idioma : |
Inglés |
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Notas : |
Article history: First Published: 11 June 2019//Correspondence: Arnau Casanovas-Massana, arnau.casanovas@yale.edu. Funding information:This research was supported by the National Institutes of Health research grants R01 AI052473, U01 AI088752, R25 TW009338, R01
TW009504 and R01 AI121207. |
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Contenido : |
ABSTRACT:
Four spirochetes (F1T, B21, YaleT and AMB6-RJ) were isolated from environmental sources: F1T and B21 from soils of an urban slum community in Salvador (Brazil), YaleT from river water in New Haven, Connecticut (USA) and AMB6-RJ from a pond in a horse farm in Rio de Janeiro (Brazil). Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus Leptospira . The analysis of 498 core genes showed that strains F1T/B21 and YaleT/AMB6-RJ formed two distinct phylogenetic clades within the ?Pathogens? group (group I). The average nucleotide identity (ANI) values of strains F1T/B21 and YaleT/AMB6-RJ to other previously described Leptospira species were below <84?%?and <82?%, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov. as new species in the genus Leptospira . The type strains are F1T (=ATCC-TSD-163=KIT0259=CLEP00287) and YaleT (=ATCC-TDS-162=KIT0258=CLEP00288), respectively. |
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Palabras claves : |
LEPTOSPIRA; LEPTOSPIRA STIMSONII SP; LEPTOSPIROSIS; PATHOGENIC; PLATAFORMA SALUD ANIMAL; SOIL; WATER; WHOLE-GENOME SEQUENCING. |
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Thesagro : |
CABALLOS; PATOGENOS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16690/1/1450-ijsem003480.pdf
https://www.microbiologyresearch.org/docserver/fulltext/ijsem/70/3/1450_ijsem003480.pdf?expires=1586896068&id=id&accname=guest&checksum=1B7512C0A3DF7B855A1C90438A02BE38
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Marc : |
LEADER 02696naa a2200385 a 4500
001 1061024
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1099/ijsem.0.003480$2DOI
100 1 $aCASANOVAS-MASSANA, A.
245 $aLeptospira yasudae sp. nov. and Leptospira stimsonii sp. nov., two new species of the pathogenic group isolated from environmental sources.$h[electronic resource]
260 $c2020
500 $aArticle history: First Published: 11 June 2019//Correspondence: Arnau Casanovas-Massana, arnau.casanovas@yale.edu. Funding information:This research was supported by the National Institutes of Health research grants R01 AI052473, U01 AI088752, R25 TW009338, R01 TW009504 and R01 AI121207.
520 $aABSTRACT: Four spirochetes (F1T, B21, YaleT and AMB6-RJ) were isolated from environmental sources: F1T and B21 from soils of an urban slum community in Salvador (Brazil), YaleT from river water in New Haven, Connecticut (USA) and AMB6-RJ from a pond in a horse farm in Rio de Janeiro (Brazil). Isolates were helix-shaped, aerobic, highly motile and non-virulent in a hamster model of infection. Draft genomes of the strains were obtained and analysed to determine the relatedness to other species of the genus Leptospira . The analysis of 498 core genes showed that strains F1T/B21 and YaleT/AMB6-RJ formed two distinct phylogenetic clades within the ?Pathogens? group (group I). The average nucleotide identity (ANI) values of strains F1T/B21 and YaleT/AMB6-RJ to other previously described Leptospira species were below <84?%?and <82?%, respectively, which confirmed that these isolates should be classified as representatives of two novel species. Therefore, we propose Leptospira yasudae sp. nov. and Leptospira stimsonii sp. nov. as new species in the genus Leptospira . The type strains are F1T (=ATCC-TSD-163=KIT0259=CLEP00287) and YaleT (=ATCC-TDS-162=KIT0258=CLEP00288), respectively.
650 $aCABALLOS
650 $aPATOGENOS
653 $aLEPTOSPIRA
653 $aLEPTOSPIRA STIMSONII SP
653 $aLEPTOSPIROSIS
653 $aPATHOGENIC
653 $aPLATAFORMA SALUD ANIMAL
653 $aSOIL
653 $aWATER
653 $aWHOLE-GENOME SEQUENCING
700 1 $aHAMOND, C.
700 1 $aSANTOS, L.A.
700 1 $aDE OLIVEIRA, D.
700 1 $aHACKER, K.P.
700 1 $aBALASSIANO, I.
700 1 $aCOSTA, F.
700 1 $aMEDEIROS, M.A.
700 1 $aREIS, M.G.
700 1 $aKO, A.I.
700 1 $aWUNDER, E.A.
773 $tInternational Journal of Systematic and Evolutionary Microbiology, 1 March 2020, Volume 70, Issue 3, Pages 1450-1456. OPEN ACCESS. Doi: https://doi.org/10.1099/ijsem.0.003480
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