02181naa a2200361 a 450000100080000000500110000800800410001902200140006002400270007410000190010124501360012026000090025650001570026552010580042265000100148065000140149065000230150465000120152765000150153965000170155465300100157165300090158165300090159070000140159970000150161370000180162870000150164670000180166170000130167970000180169270000270171077300820173710501912019-10-08       2010    bl uuuu     u00u1 u    #d  a0021-85617 a10.1021/jf90432592DOI1 aCARLOMAGNO, M.  aA clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture.h[electronic resource]  c2010  aArticle history:  received for review December 7, 2009. Revised manuscript received March 8, 2010. Accepted March 10, 2010. Published on Web 03/19/2010.  aAbstract: The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzymelinked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0-20 ng/mL range in field samples, with excellent correlation with highperformance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment.  aARROZ  aCLOMAZONE  aCONTROL DE MALEZAS  aESCARDA  aHERBICIDAS  aORYZA SATIVA  aELISA  aHPLC  aRICE1 aMATHO, C.1 aCANTOU, G.1 aSANBORN, J.R.1 aLAST, J.A.1 aHAMMOCK, B.D.1 aROEL, A.1 aGONZÁLEZ, D.1 aGONZÁLEZ-SAPIENZA, G.  tJournal of Agricultural and Food Chemistry, 2010gv. 58, no.7,  p. 4367-4371.