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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/09/2023 |
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Actualizado : |
22/09/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BERAIS-RUBIO, A.; MOREL-REVETRIA, M.; FILIPPI, C.V.; REYNO, R.; MONZA, J. |
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Afiliación : |
ANDRÉS BERAIS-RUBIO, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; MARÍA MOREL-REVETRIA, Laboratorio de Microbiología de Suelos (LMS), Facultad de Ciencias, Universidad de la República, Iguá 4225, Montevideo PC 11.400, Uruguay; CARLA VALERIA FILIPPI, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay; RAFAEL ALEJANDRO REYNO PODESTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE MONZA, Departamento de Biología Vegetal, Laboratorio de Bioquímica, Universidad de la República, Av. Garzón 809, Montevideo PC 12.900, Uruguay. |
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Título : |
Ensifer meliloti elite strain U143 used as alfalfa inoculant in Uruguay: Characterization and draft genome sequence. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
The Microbe. 2023, Volume 1, article 100008. https://doi.org/10.1016/j.microb.2023.100008 -- OPEN ACCESS. |
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ISSN : |
2950-1946 |
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DOI : |
10.1016/j.microb.2023.100008 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 23 May 2023, Revised 14 August 2023, Accepted 12 September 2023, Available online 13 September 2023, Version of Record 19 September 2023. -- Correspondence: Jorge Monza, mail: jmonza@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). -- FUNDING: This work was financially supported by Instituto Nacional de
Investigación Agropecuaria (INIA) L-1, Grant Comisión Académica de Postgrado, Universidad de la República, Programa de Desarrollo de
Ciencias Básicas (PEDECIBA) and Sistema Nacional de Investigadores (SNI). |
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Contenido : |
ABSTRACT.- Ensifer (syn. Sinorhizobium) meliloti U143 is an effective nitrogen-fixing strain isolated from Uruguayan soils. For decades, this strain has been used as an inoculant for different alfalfa cultivars. Here we report for the first time a characterization of the U143 elite strain that includes the preliminary genomic sequence, its annotation, and physiological parameters related to its symbiotic efficiency and nitrate respiration capacity. Through Illumina sequencing, the genome of the U143 strain was sequenced. The genome length was 6,801,966 bp, and it contained two megaplasmids, an average GC content of 62.15 %, and 6522 protein-coding sequences. In the symbiotic plasmid, we identified nap, nir, nor, and nos sequences that explain the ability of the U143 strain to respire NO3- in free-living and microaerobic conditions. Field assays performed in two locations for two years showed that alfalfa inoculated with the U143 strain produced 41 % more total shoot dry matter than the non-inoculated control, and between 61.3 % and 66.5 % of shoot N in alfalfa inoculated with strain U143 derived from nitrogen fixation. © 2023 The Authors. Published by Elsevier Ltd |
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Palabras claves : |
Draft genome; Elite strain; Inoculant. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.sciencedirect.com/science/article/pii/S2950194623000080/pdf
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Marc : |
LEADER 02580naa a2200241 a 4500
001 1064314
005 2023-09-22
008 2023 bl uuuu u00u1 u #d
022 $a2950-1946
024 7 $a10.1016/j.microb.2023.100008$2DOI
100 1 $aBERAIS-RUBIO, A.
245 $aEnsifer meliloti elite strain U143 used as alfalfa inoculant in Uruguay$bCharacterization and draft genome sequence.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 23 May 2023, Revised 14 August 2023, Accepted 12 September 2023, Available online 13 September 2023, Version of Record 19 September 2023. -- Correspondence: Jorge Monza, mail: jmonza@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/). -- FUNDING: This work was financially supported by Instituto Nacional de Investigación Agropecuaria (INIA) L-1, Grant Comisión Académica de Postgrado, Universidad de la República, Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Sistema Nacional de Investigadores (SNI).
520 $aABSTRACT.- Ensifer (syn. Sinorhizobium) meliloti U143 is an effective nitrogen-fixing strain isolated from Uruguayan soils. For decades, this strain has been used as an inoculant for different alfalfa cultivars. Here we report for the first time a characterization of the U143 elite strain that includes the preliminary genomic sequence, its annotation, and physiological parameters related to its symbiotic efficiency and nitrate respiration capacity. Through Illumina sequencing, the genome of the U143 strain was sequenced. The genome length was 6,801,966 bp, and it contained two megaplasmids, an average GC content of 62.15 %, and 6522 protein-coding sequences. In the symbiotic plasmid, we identified nap, nir, nor, and nos sequences that explain the ability of the U143 strain to respire NO3- in free-living and microaerobic conditions. Field assays performed in two locations for two years showed that alfalfa inoculated with the U143 strain produced 41 % more total shoot dry matter than the non-inoculated control, and between 61.3 % and 66.5 % of shoot N in alfalfa inoculated with strain U143 derived from nitrogen fixation. © 2023 The Authors. Published by Elsevier Ltd
653 $aDraft genome
653 $aElite strain
653 $aInoculant
700 1 $aMOREL-REVETRIA, M.
700 1 $aFILIPPI, C.V.
700 1 $aREYNO, R.
700 1 $aMONZA, J.
773 $tThe Microbe. 2023, Volume 1, article 100008. https://doi.org/10.1016/j.microb.2023.100008 -- OPEN ACCESS.
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
23/01/2023 |
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Actualizado : |
27/04/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASAUX, M.L.; NETO, W.S.; SCHILD, C.; COSTA, R.A. DA; MACÍAS-RIOSECO, M.; CAFFARENA, D.; SILVEIRA, C.S.; ARÁOZ, V.; DONCEL, B.; GIANNITTI, F.; FRAGA, M. |
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Afiliación : |
MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; WALDEMIR SANTIAGO NETO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ALMEIDA DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; California Animal Health and Food Safety (CAHFS) Laboratory, Tulare Branch, University of California at Davis, Tulare, CA, USA; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Unidad Académica Salud de los Rumiantes, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BENJAMÍN DONCEL DÍAZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Laboratorio de Patología Veterinaria Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Epidemiological and clinicopathological findings in 15 fatal outbreaks of salmonellosis in dairy calves and virulence genes in the causative Salmonella enterica Typhimurium and Dublin strains. |
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Complemento del título : |
Veterinary Microbiology - Research Paper. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Brazilian Journal of Microbiology, 2023, volume 54, isuue 1, pp. 475-490. doi: https://doi.org/10.1007/s42770-022-00898-9 |
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ISSN : |
1517-8382 |
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DOI : |
10.1007/s42770-022-00898-9 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 06 December 2021; Accepted 20 December 2022; Published 05 January 2023. -- Correspondence author: Fraga, M.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Ruta 50, Km 11, El Semillero, Uruguay; email:mfraga@inia.org.uy -- Funding: This work was partially funded by INIA's grant PL-15, project FMV-104922 from the Uruguayan "Agencia Nacional de Investigación e Innovación" (ANII) and ANII (POS_NAC_2016_1_130296). -- Responsible Editor: Luiz Henrique Rosa. Supplementary information: https://static-content.springer.com/esm/art%3A10.1007%2Fs42770-022-00898-9/MediaObjects/42770_2022_898_MOESM1_ESM.xlsx |
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Contenido : |
ABSTRACT.- Salmonella enterica is a major food-borne pathogen that affects cattle-rearing systems worldwide. Little information is available on the epidemiology and pathology of salmonellosis and the virulence genes (VGs) carried by Salmonella in spontaneous outbreaks in cattle. We describe epidemiological findings in 15 fatal outbreaks of salmonellosis in Uruguayan dairy farms and the age, clinical signs, and pathology in 20 affected calves. We also describe the serotypes and frequencies of 17 VGs in the causative Salmonella strains and explore their associations with epidemiological, clinical, and pathological findings. Salmonella Typhimurium and Dublin were identified in 11/15 and 4/15 outbreaks, respectively. The most frequent reason for consultation was digestive disease (8 outbreaks caused by S. Typhimurium), followed by sudden death (4 outbreaks, 3 caused by S. Dublin). © 2023, The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia. |
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Palabras claves : |
Calves; Epidemiology; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Salmonella enterica; Uruguay; Virulence genes. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 02878naa a2200361 a 4500
001 1063946
005 2023-04-27
008 2023 bl uuuu u00u1 u #d
022 $a1517-8382
024 7 $a10.1007/s42770-022-00898-9$2DOI
100 1 $aCASAUX, M.L.
245 $aEpidemiological and clinicopathological findings in 15 fatal outbreaks of salmonellosis in dairy calves and virulence genes in the causative Salmonella enterica Typhimurium and Dublin strains.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 06 December 2021; Accepted 20 December 2022; Published 05 January 2023. -- Correspondence author: Fraga, M.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Ruta 50, Km 11, El Semillero, Uruguay; email:mfraga@inia.org.uy -- Funding: This work was partially funded by INIA's grant PL-15, project FMV-104922 from the Uruguayan "Agencia Nacional de Investigación e Innovación" (ANII) and ANII (POS_NAC_2016_1_130296). -- Responsible Editor: Luiz Henrique Rosa. Supplementary information: https://static-content.springer.com/esm/art%3A10.1007%2Fs42770-022-00898-9/MediaObjects/42770_2022_898_MOESM1_ESM.xlsx
520 $aABSTRACT.- Salmonella enterica is a major food-borne pathogen that affects cattle-rearing systems worldwide. Little information is available on the epidemiology and pathology of salmonellosis and the virulence genes (VGs) carried by Salmonella in spontaneous outbreaks in cattle. We describe epidemiological findings in 15 fatal outbreaks of salmonellosis in Uruguayan dairy farms and the age, clinical signs, and pathology in 20 affected calves. We also describe the serotypes and frequencies of 17 VGs in the causative Salmonella strains and explore their associations with epidemiological, clinical, and pathological findings. Salmonella Typhimurium and Dublin were identified in 11/15 and 4/15 outbreaks, respectively. The most frequent reason for consultation was digestive disease (8 outbreaks caused by S. Typhimurium), followed by sudden death (4 outbreaks, 3 caused by S. Dublin). © 2023, The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.
653 $aCalves
653 $aEpidemiology
653 $aPathology
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aSalmonella enterica
653 $aUruguay
653 $aVirulence genes
700 1 $aNETO, W.S.
700 1 $aSCHILD, C.
700 1 $aCOSTA, R.A. DA
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aCAFFARENA, D.
700 1 $aSILVEIRA, C.S.
700 1 $aARÁOZ, V.
700 1 $aDONCEL, B.
700 1 $aGIANNITTI, F.
700 1 $aFRAGA, M.
773 $tBrazilian Journal of Microbiology, 2023, volume 54, isuue 1, pp. 475-490. doi: https://doi.org/10.1007/s42770-022-00898-9
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