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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
22/01/2020 |
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Actualizado : |
24/02/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RODRIGO, M.J.; LADO, J.; ALÓS, E.; ALQUÉZAR, B.; DERY, O.; HIRSCHBERG, J.; ZACARÍAS, L. |
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Afiliación : |
MARÍA JESÚS RODRIGO, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; ENRIQUETA ALÓS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; BERTA ALQUÉZAR, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain; Instituto de Biología Molecular y Celular de Plantas (IBMCP), UPV-CSIC, Spain; ORLY DERY, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; JOSEPH HIRSCHBERG, Alexander Silberman Institute of Life Sciences, Hebrew University of Jerusalem, Jerusalem, Israel; LORENZO ZACARÍAS, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Spain. |
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Título : |
A mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
BMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2 |
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ISSN : |
1471-2229 |
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DOI : |
10.1186/s12870-019-2078-2 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019.
Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).
Supplementary information accompanies this paper. |
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Contenido : |
Abstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits.
© 2019 The Author(s). MenosAbstract.
Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit an... Presentar Todo |
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Palabras claves : |
Carotenoid; GENE EXPRESSION; MUTANT; Pigmentation; PLATAFORMA AGROALIMENTOS; RIPENING; Zeta-carotene isomerase. |
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Thesagro : |
CAROTENOIDES; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14051/1/BMC-Plant-Pathology-2019-s12870-019-2078-2.pdf
https://bmcplantbiol.biomedcentral.com/track/pdf/10.1186/s12870-019-2078-2
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Marc : |
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022 $a1471-2229
024 7 $a10.1186/s12870-019-2078-2$2DOI
100 1 $aRODRIGO, M.J.
245 $aA mutant allele of ζ-carotene isomerase (Z-ISO) is associated with the yellow pigmentation of the "pinalate" sweet orange mutant and reveals new insights into its role in fruit carotenogenesis.$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 17 June 2019 / Accepted: 16 October 2019 / Published online: 4 November 2019. Funding text: Financial support of the research grants AGL2012?34576 and AGL2015? 70218 (Ministerio Economía y Competitividad, Spain), and RTI2018-095131-BI00 (Ministerio Ciencia, Innovacion y Universidades, Spain). Work in the group of JH was supported by Israel Science Foundation Grant 850/13. MJR, LZ, JH and OD are members of Eurocaroten (COST_Action CA15136). MJR and LZ belong to CaRed (Spanish Carotenoid Network BIO2017?90877-REDT, Minis-terio de Ciencia, Innovación y Universidades, Spain). Authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Supplementary information accompanies this paper.
520 $aAbstract. Background: Fruit coloration is one of the main quality parameters of Citrus fruit primarily determined by genetic factors. The fruit of ordinary sweet orange (Citrus sinensis) displays a pleasant orange tint due to accumulation of carotenoids, representing β,β-xanthophylls more than 80% of the total content. 'Pinalate' is a spontaneous bud mutant, or somatic mutation, derived from sweet orange 'Navelate', characterized by yellow fruits due to elevated proportions of upstream carotenes and reduced β,β-xanthophylls, which suggests a biosynthetic blockage at early steps of the carotenoid pathway. Results: To identify the molecular basis of 'Pinalate' yellow fruit, a complete characterization of carotenoids profile together with transcriptional changes in carotenoid biosynthetic genes were performed in mutant and parental fruits during development and ripening. 'Pinalate' fruit showed a distinctive carotenoid profile at all ripening stages, accumulating phytoene, phytofluene and unusual proportions of 9,15,9′-tri-cis- and 9,9′-di-cis-ζ-carotene, while content of downstream carotenoids was significantly decreased. Transcript levels for most of the carotenoid biosynthetic genes showed no alterations in 'Pinalate'; however, the steady-state level mRNA of ζ-carotene isomerase (Z-ISO), which catalyses the conversion of 9,15,9′-tri-cis- to 9,9′-di-cis-ζ-carotene, was significantly reduced both in 'Pinalate' fruit and leaf tissues. Isolation of the 'Pinalate' Z-ISO genomic sequence identified a new allele with a single nucleotide insertion at the second exon, which generates an alternative splicing site that alters Z-ISO transcripts encoding non-functional enzyme. Moreover, functional assays of citrus Z-ISO in E.coli showed that light is able to enhance a non-enzymatic isomerization of tri-cis to di-cis-ζ-carotene, which is in agreement with the partial rescue of mutant phenotype when 'Pinalate' fruits are highly exposed to light during ripening. Conclusion: A single nucleotide insertion has been identified in 'Pinalate' Z-ISO gene that results in truncated proteins. This causes a bottleneck in the carotenoid pathway with an unbalanced content of carotenes upstream to β,β-xanthophylls in fruit tissues. In chloroplastic tissues, the effects of Z-ISO alteration are mainly manifested as a reduction in total carotenoid content. Taken together, our results indicate that the spontaneous single nucleotide insertion in Z-ISO is the molecular basis of the yellow pigmentation in 'Pinalate' sweet orange and points this isomerase as an essential activity for carotenogenesis in citrus fruits. © 2019 The Author(s).
650 $aCAROTENOIDES
650 $aCITRUS
653 $aCarotenoid
653 $aGENE EXPRESSION
653 $aMUTANT
653 $aPigmentation
653 $aPLATAFORMA AGROALIMENTOS
653 $aRIPENING
653 $aZeta-carotene isomerase
700 1 $aLADO, J.
700 1 $aALÓS, E.
700 1 $aALQUÉZAR, B.
700 1 $aDERY, O.
700 1 $aHIRSCHBERG, J.
700 1 $aZACARÍAS, L.
773 $tBMC Plant Biology, 4 November 2019, Volume 19, Issue 1, Article number 465. OPEN ACCESS. Doi: DOI: 10.1186/s12870-019-2078-2
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| 3. |  | GRATTAROLA, M.; PÉREZ JONES, J. Día del merino 2004. ln: INIA Tacuarembó. Sociedad Criadores Merino Australiano del Uruguay. SUL. Proyecto Merino Fino del Uruguay: cuarta distribución de carneros generados en el Núcleo Fundacional de Merino Fino de la la Unidad Experimental Glencoe, 1999 - 2003. Glencoe, Paysandú, 10 de diciembre, 2003. Tacuarembó (Uruguay): INIA, 2003. p. 72-74 (INIA Serie Actividades de Difusión ; 343)| Biblioteca(s): INIA Tacuarembó. |
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| 10. | | MONTOSSI, F.; DE MATTOS, D.; MEDEROS, A.; DE BARBIERI, I.; GRATTAROLA, M.; PÉREZ JONES, J. Avances del Proyecto Merino Fino del Uruguay, obtenidos en el núcleo fundacional de la Unidad Experimental Glencoe de INIA Tacuarembó El País Agropecuario, 2001, v. 6, no. 73, p. 25-28.| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Tacuarembó. |
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| 11. | | ABELLA, I.; JAURENA, M.; RAMOS, Z.; PREVE, F.; GRATTAROLA, M.; DE BARBIERI, I. Experiencias en la producción de lanas finas en sistemas comerciales del Basalto. In: DE BARBIERI, I.; RAMOS, Z.; MONTOSSI, F. (Ed.). Lana superfina: un camino conjunto de la investigación, la transferencia y la producción. Montevideo (Uruguay): INIA, 2018. p. 11-30 (INIA Serie Técnica; 242)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 12. | | CIAPPESONI, G.; GIMENO, D.; RAVAGNOLO, O.; DE BARBIERI, I.; MONTOSSI, F.; GRATTAROLA, M.; MEDEROS, A. Evaluación genética del núcleo fundacional: caracterización de los animales que se entregan. ln: INIA Tacuarembó. Unidad Experimental Glencoe. Proyecto Merino Fino del Uruguay: sexta distribución de carneros generados en el núcleo fundacional de merino fino de la Unidad Experimental Glencoe, INIA Tacuarembó, 1999 - 2005. 16 diciembre, Glencoe, Paysandú, 2005. Tacuarembó (Uruguay): INIA, 2005. p. 49-74 (INIA Serie Actividades de Difusión ; 439)| Biblioteca(s): INIA Tacuarembó. |
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| 13. | | CIAPPESONI, G.; GIMENO, D.; DE BARBIERI, I.; MONTOSSI, F.; GRATTAROLA, M.; MEDEROS, A. Evaluación genética poblacional: Caracterización de los animales del Núcleo que se entregan (475). In: INIA Tacuarembó. Unidad Experimental Glencoe. Avances obtenidos en el Proyecto Merino Fino del Uruguay: núcleo fundacional Unidad Experimental Glencoe, 1999-2006. Paysandú, 19 diciembre, 2006. Tacuarembó (Uruguay): INIA, 2006. p. 54-78 (INIA Serie Actividades de Difusión ; 475)| Biblioteca(s): INIA Tacuarembó. |
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| 14. | | CIAPPESONI, G.; GIMENO, D.; DE BARBIERI, I.; MONTOSSI, F.; GRATTAROLA, M.; MEDEROS, A. Evaluación genética poblacional: caracterización de los animales del Núcleo que se entregan. ln: INIA Tacuarembó. Estación Experimental Glencoe. Proyecto Merino Fino del Uruguay: octava distribución de carneros generados en el núcleo fundacional de Merino fino de la Unidad Experimental Glencoe, 1999-2007, Paysandú, 13 de diciembre, 2006. Tacuarembó (Uruguay): INIA, 2007. p. 8-33 (INIA Serie Actividades de Difusión ; 523)| Biblioteca(s): INIA Tacuarembó. |
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| 15. | | CIAPPESONI, G.; GIMENO, D.; RAVAGNOLO, O.; DE BARBIERI, I.; AGUILAR, I.; MONTOSSI, F.; GRATTAROLA, M. Evaluación genética preliminar del Núcleo Fundacional Merino Fino: análisis combinado población Merino fino - generación 2003. ln: INIA Tacuarembó. Sociedad Criadores Merino Australiano del Uruguay. SUL. Proyecto Merino Fino del Uruguay: quinta distribución de carneros generados en el núcleo fundacional de merino fino de la Unidad Experimental Glencoe, INIA Tacuarembó, 1999 - 2004. Glencoe, Paysandú, 10 diciembre, 2004. Tacuarembó (Uruguay): INIA, 2004. p. 69-83 (INIA Serie Actividades de Difusión ; 392)| Biblioteca(s): INIA Tacuarembó. |
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| 18. | | ABELLA, I.; GRATTAROLA, M.; JAURENA, M.; DE BARBIERI, I.; RAMOS, Z.; PREVE, F. Producción de lanas finas, una alternativa validada en sistemas comerciales. Revista INIA Uruguay, 2017, no.51, p.15-19. (Revista INIA; 51)| Tipo: Artículos en Revistas Agropecuarias |
| Biblioteca(s): INIA Tacuarembó. |
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| 19. | | DE BARBIERI, I.; MONTOSSI, F.; NOLLA, M.; LUZARDO, S.; GRATTAROLA, M.; PÉREZ JONES, J.; FROS, A.; MEDEROS, A.; DONAGARAY, F. Encuesta de opinión sobre los avances logrados en el Proyecto Merino Fino - Fase I (período 1998 - 2004). ln: INIA Tacuarembó. Unidad Experimental Glencoe. Proyecto Merino Fino del Uruguay: sexta distribución de carneros generados en el núcleo fundacional de merino fino de la Unidad Experimental Glencoe, INIA Tacuarembó, 1999 - 2005. 16 diciembre, Glencoe, Paysandú, 2005. Tacuarembó (Uruguay): INIA, 2005. p. 39-48 (INIA Serie Actividades de Difusión ; 439)| Biblioteca(s): INIA Tacuarembó. |
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| 20. | | DE BARBIERI, I.; MONTOSSI, F.; NOLLA, M.; LUZARDO, S.; GRATTAROLA, M.; PÉREZ JONES, M.; FROS, A.; MEDEROS, A.; DONAGARAY, F. V. Encuesta de opinión sobre los avances logrados en el proyecto Merino Fino - Fase I (Período 1998-2004) ln: MONTOSSI, F.; DE BARBIERI, I. (Eds). Proyecto Merino Fino del Uruguay: una visión con perspectiva histórica. Montevideo (Uruguay): INIA, 2007. p. 95-103 (INIA Boletín de Divulgación ; 90)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Las Brujas; INIA Tacuarembó. |
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| Registros recuperados : 54 | |
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