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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
07/10/2014 |
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Actualizado : |
05/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ALARCÓN-REVERTE, R.; GARCIA, M.A.; WATSON, S.B.; ABDALLAH, I.; SABATÉ, S.; HERNÁNDEZ, M.J.; DAYAN ,F.E.; FISCHER, A.J. |
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Afiliación : |
MILTON ALEJANDRO GARCIA LATASA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Concerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Pest Management Science, v. 71, n. 7, p. 996-2007, 2015. |
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ISSN : |
1526-4998. |
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DOI : |
10.1002/ps.3878 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014 |
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Contenido : |
Abstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different populations may carry different resistance mechanisms and different mechanisms can
act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant
expression of EPSPS sensitivity to glyphosate. MenosAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population
carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide,
was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected
glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of
California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance.
RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than
S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption,
translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to
Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was
found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants.
Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants.
CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same
population, individuals from different popula... Presentar Todo |
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Palabras claves : |
5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE; EPSPS EXPRESSION; EPSPS INHIBITION ASSAY; POLYPLOIDY; RESISTANCE MECHANISM. |
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Thesagro : |
RESISTENCIA A GLIFOSATO. |
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Asunto categoría : |
H60 Malezas y escardas |
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Marc : |
LEADER 02883naa a2200313 a 4500
001 1050961
005 2019-11-05
008 2015 bl uuuu u00u1 u #d
022 $a1526-4998.
024 7 $a10.1002/ps.3878$2DOI
100 1 $aALARCÓN-REVERTE, R.
245 $aConcerted action of target-site mutations and high EPSPS activity in glyphosate-resistant junglerice (Echinochloa colona) from California.$h[electronic resource]
260 $c2015
500 $aArticle history: Received: 4 April 2014 Revised: 31 July 2014/Accepted article published: 13 August 2014 /Published online in Wiley Online Library: 5 September 2014
520 $aAbstract:BACKGROUND: Echinochloa colona is an annual weed affecting field crops and orchards in California. An E. colona population carrying a mutation in the EPSPS gene endowing resistance to glyphosate, the most widely used non-selective herbicide, was recently identified in the Northern Sacramento Valley of California. Plants from this population, from a suspected glyphosate-resistant (GR) population, and from one susceptible (S) population collected in the Northern Sacramento Valley of California, were used to generate three GR and one S selfedlines to study possiblemechanismsinvolved in glyphosate resistance. RESULTS: Based on the amount of glyphosate required to kill 50% of the plants (LD50), GR lines were 4?9-fold more resistant than S plants and accumulated less shikimate after glyphosate treatment. GR and S lines did not differ in glyphosate absorption, translocation or metabolism. A different target-site mutation was found in each of two of the GR lines corresponding to Pro106Thr and Pro106Ser substitutions; the mutations were found in different homoeologous EPSPS genes. No mutation was found in the third GR line, which exhibited 1.4-fold higher basal EPSPS activity and a fivefold greater LD50 than S plants. Quantitative RT-PCR revealed that GR lines had similar or lower EPSPS expression than S plants. CONCLUSION: It is demonstrated that individuals with different glyphosate resistance mechanisms can coexist in the same population, individuals from different populations may carry different resistance mechanisms and different mechanisms can act in concert within single E. colona plants. However, other plant factors or resistance mechanisms appear to modulate plant expression of EPSPS sensitivity to glyphosate.
650 $aRESISTENCIA A GLIFOSATO
653 $a5-ENOLPYRUVYLSHIKIMATE-3-PHOSPHATE SYNTHASE
653 $aEPSPS EXPRESSION
653 $aEPSPS INHIBITION ASSAY
653 $aPOLYPLOIDY
653 $aRESISTANCE MECHANISM
700 1 $aGARCIA, M.A.
700 1 $aWATSON, S.B.
700 1 $aABDALLAH, I.
700 1 $aSABATÉ, S.
700 1 $aHERNÁNDEZ, M.J.
700 1 $aDAYAN ,F.E.
700 1 $aFISCHER, A.J.
773 $tPest Management Science$gv. 71, n. 7, p. 996-2007, 2015.
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