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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
26/01/2024 |
Actualizado : |
26/01/2024 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
Complemento del título : |
Original full article. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
ISSN : |
1983-0246 |
DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
Idioma : |
Inglés |
Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additio... Presentar Todo |
Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
Asunto categoría : |
L10 Genética y mejoramiento animal |
URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03124naa a2200361 a 4500 001 1064440 005 2024-01-26 008 2023 bl uuuu u00u1 u #d 022 $a1983-0246 024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI 100 1 $aPICCOLI, R.J. 245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource] 260 $c2023 500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- 520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. 653 $aDiagnosis 653 $aImmunohistochemistry 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aPsittacosis 653 $aZoonosis 700 1 $aGRUCHOUSKEI, L. 700 1 $aBENINCA, A.L.V. 700 1 $aMAURENTE BERÓN, M. 700 1 $aCHENG, A.C. 700 1 $aDE ANDRADE, J.A. 700 1 $aFACCIN, M. 700 1 $aGRZEGOZEVSKI, A.P. 700 1 $aDA SILVA, G.C.R. 700 1 $aFRANÇA, J.C. 700 1 $aFERNANDES, N.L.M. 700 1 $aDE CARVALHO, A.L. 700 1 $aVIOTT, A.M. 773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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Registros recuperados : 686 | |
1. | | SOUSA, A.L.V.; SOUSA, D.E.R.; CERQUEIRA, L.A.; QUEIROZ-MACHADO, C.R.R.; GIARETTA, P.R.; RIET-CORREA, F.; CASTRO, M.B.; MACHADO, M. Cestrum axillare hepatotoxicity in cattle in the Distrito Federal, Goiás and Minas Gerais. [Hepatotoxicidade do Cestrum axillare em bovinos do Distrito Federal, Goiás e Minas Gerais].. Original Article. Livestock Diseases. Pesquisa Veterinaria Brasileira. 2024, Volume 44, e07399. https://doi.org/10.1590/1678-5150-PVB-7399 -- OPEN ACCESS. Article history: Received 08 Nov 2023, Accepted 09 Dec 2023, Publication in this collection 18 Mar 2024, Date of issue 2024. -- Correspondence: Machado, M.; Graduate Program in Animal Science, Faculdade de Agronomia e Medicina...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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2. | | FASIOLO, C.; VIETA, A.; ORIHUELA, C.; WATERSTON, S.; IURATO, A.; PRIMO, P.; MARTÍNEZ, F.; GODÍN, A.; VIANA, V.; BAO, L.; LORENZO, E.; GALVÁN, G. FPTA 383: Programa de integración de herramientas biológicas y culturales para el control de plagas y enfermedades en horticultura. Proyectos FPTA. Revista INIA Uruguay, Marzo 2024, no.76, p.86-88. (Revista INIA; 76).Tipo: Artículos en Revistas Agropecuarias |
Biblioteca(s): INIA Las Brujas. |
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3. | | GIANNITTI, F.; MACHADO, M.; SILVEIRA, C.S.; CIBILS-STEWART, X.; BARAIBAR, N.; QUEIROZ-MACHADO, C.R.R.; POPPENGA, R.H.; MENCHACA, A.; UZAL, F.A.; GARCÍA, J.A.; MATTO, C.; DUTRA, F.; RUPRECHTER, G.; CAFFARENA, D.; SARAVIA, A. Experimental oral administration of pollen beetle (Astylus atromaculatus) to cattle results in an acute lethal gastrointestinal disease. Research article. Veterinary Pathology. 2024. https://doi.org/10.1177/03009858241231557 -- [In Press] Article history: First published online March 4, 2024. -- Correspondence: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria, Estación Experimental La Estanzuela,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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4. | | COSTA, M.; MANSILLA, F.; MANUEL SALA, J.; SARAVIA, A.; UBIOS, D.; LORES, P.; CAPOZZO, A.V.; FREIRE, T. Fasciola hepatica infection modifies lgG1 specific immune response to foot-and-mouth disease virus induced by vaccination. Vaccine. 2024, Volume 42, Issue 3, Pages 541-547. https://doi.org/10.1016/j.vaccine.2023.12.067 Article history: Received 19 August 2023; Received in revised form 15 December 2023; Accepted 20 December 2023; Available online 6 January 2024. -- Correspondence: Freire, T.; UdelaR, Facultad de Medicina, Departamento de Inmunobiología,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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5. | | MACÊDO, I.L. DE; SOUSA, D.E.R. DE; SOUZA, A.D.R.; ARMIÉN, A.G.; MACHADO, M.; CASTRO, M.B. DE Fatal Africanized bee attack in captive non-human primates (Saimiri ustus and Sapajus libidinosus). Short communication. Toxicon. 2024, Volume 241, Article 107666. https://doi.org/10.1016/j.toxicon.2024.107666 Article history: Received 8 February 2024, Revised 26 February 2024, Accepted 27 February 2024, Available online 28 February 2024, Version of Record 8 March 2024. -- Correspondence: Castro, M.B.D.; Veterinary Pathology and Forensic...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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9. | | MACHADO, M.; QUEIROZ-MACHADO, C.R.R.; GARDNER, D.R.; CASTRO, M.B.; CÂMARA, A.C.L.; PIMENTEL, L.A.; GALIZA, G.J.N.; RIET-CORREA, F. Leucaena leucocephala toxicity in Brazilian horses. Toxicon. 2024, Volume 240, Article 107655. https://doi.org/10.1016/j.toxicon.2024.107655 Article history: Received 10 January 2024; Received in revised form 16 February 2024; Accepted 18 February 2024; Available online 19 February 2024. -- Correspondence: Machado, M.; Plataforma de Investigación en Salud Animal (PSA),...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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10. | | PARODI, P.; BAZZANO, V.; ARMÚA-FERNÁNDEZ, M.T.; FÉLIX, M.L.; CARVALHO, L.A.; FREIRE, J.; VENZAL, J.M. Molecular survey of Piroplasmida, Hepatozoon spp. and Anaplasmataceae in anemic and thrombocytopenic dogs from Uruguay. Original article. Veterinary Parasitology: Regional Studies and Reports. 2024, Volume 51, 101027. https://doi.org/10.1016/j.vprsr.2024.101027 Article history: Received 28 February 2024, Revised 8 April 2024, Accepted 17 April 2024, Available online 21 April 2024, Version of Record 23 April 2024. -- Corresponding author at: Rivera 1350, CP 50000 Salto, Uruguay. E-mail address:...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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13. | | GARCÍA, J.A.; LIVIO, J.M.; MATTO, C.; DUTRA, F.; SCIOLI, V.; GIANNITTI, F.; LANGSTON, J.; POPPENGA, R.H.; CANTÓN, G.J.; UZAL, F.A. Pollen beetle (Astylus atromaculatus)-associated gastroenteric disease in cattle: report of 6 natural outbreaks. Full Scientific Report. Journal of Veterinary Diagnostic Investigation, 2024, Volume 36, Issue 1, Pages 95-102. https://doi.org/10.1177/10406387231215756 Article history: Article first published online: November 29, 2023; Issue published: January 2024. -- Correspondence: Uzal, F.A.; California Animal Health and Food Safety Laboratory, San Bernardino branches, School of Veterinary Medicine,...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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17. | | MENCHACA, A. Assisted Reproductive Technologies (ART) and genome editing to support a sustainable livestock. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230074. http://dx.doi.org/10.1590/1984-3143-AR2023-0074 -- OPEN ACCESS. Article history: Received May 26, 2023; Accepted July 21, 2023. -- Document type: Article Gold Open Access, Green Open Access. -- Correspondence: Menchaca, A.; Plataforma de Salud Animal, Instituto Nacional de Investigación...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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19. | | PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. Original full article. Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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20. | | GIANNITTI, F.; DORSCH, M.; FERNÁNDEZ-CIGANDA, S.; RABAZA, A.; VÁZQUEZ, S.; CÉSAR, C.; HURTADO, J.; GREIF, G.; RABENECK, D.; BHATNAGAR, J.; RITTER, J. Canine leproid granuloma caused by a member of the Mycobacterium tuberculosis complex. (Brief Report). Journal of Veterinary Diagnostic Investigation. 2023, Volume 35, Issue 4, pages 439-443. https://doi.org/10.1177/10406387231176816 Article history: First published online May 19, 2023; Issue published July 2023. -- Corresponding author: Federico Giannitti, Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 686 | |
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