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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
03/08/2021 |
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Actualizado : |
03/08/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MARTÍNEZ, R.; PALLADINO, D.A.; BANCHERO, G.; FERNÁNDEZ-MARTIN, R.; NANNI, M.; JULIANO, N.; LORIO, J.; LA MANNA, A. |
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Afiliación : |
ROCÍO MARTÍNEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL ALEJANDRO PALLADINO, Instituto de Producción Animal (INPA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina C1417DSE; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RAFAEL FERNÁNDEZ-MARTIN, Instituto de Producción Animal (INPA), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina C1417DSE.; MARIANA NANNI, Instituto de Investigación Tecnología de los Alimentos?Instituto Nacional de Tecnología Agropecuaria, Buenos Aires, Argentina B1686.; NICOLÁS JULIANO, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina C1417DSE.; JESICA LORIO, Facultad de Agronomía, Universidad de Buenos Aires, Buenos Aires, Argentina C1417DSE.; ALEJANDRO FRANCISCO LA MANNA ALONSO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Providing heat-stress abatement to late-lactation Holstein cows affects hormones, metabolite blood profiles, and hepatic gene expression but not productive responses. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Applied Animal Science Volume 37, Issue 4, August 2021, Pages 490-503. OPEN ACCESS. Doi: https://doi.org/10.15232/aas.2020-02109 |
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DOI : |
10.15232/aas.2020-02109 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 7 October 2020, Accepted 7 June 2021, Available online 28 July 2021. Corresponding author: rmartine@agro.uba.ar.
This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed. |
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Contenido : |
ABSTRACT: Objective: Our objective was to evaluate the effects of providing shade and shade combined with evaporative cooling on production, cow activity, metabolism, and hepatic gene expression of late-lactation Holstein dairy cows under moderate heat-stress conditions. Materials and Methods: Forty-eight multiparous Holstein cows were used in a completely randomized blockdesign trial and randomly assigned to 1 of 3 treatments: control (CTL), without access to shade; access to artificial shade (SH); and shade combined with evaporative cooling (SHplus). Results were analyzed using a mixed procedure
with repeated measures. Results and Discussion: No differences were observed in DMI. Milk yield was not different among treatments, but lactose concentration was greater in SHplus. Treatments CTL and SH had greater BW losses than SHplus. Control cows spent less time grazing. The CTL and SH had higher p.m. rectal temperatures than SHplus, whereas CTL had the highest p.m. respiration rate. Control cows had greater serum insulin levels. Control and SH had
greater BHB and urea concentrations and lower glucose concentration compared with SHplus. The hepatic expression of PCK1, PDK4, and HP genes was downregulated in SH and SHplus relative to control. Hepatic expression of NFKB1 was downregulated, whereas SOCS2 was upregulated, for SHplus compared with CTL.Implications and Applications: Despite the absence of treatment effects on productive variables, changes in blood profiles and hepatic expression of target genes were observed among treatments. These results suggest that the provision of shade combined with evaporative cooling was effective in alleviating the negative effects of heat stress. MenosABSTRACT: Objective: Our objective was to evaluate the effects of providing shade and shade combined with evaporative cooling on production, cow activity, metabolism, and hepatic gene expression of late-lactation Holstein dairy cows under moderate heat-stress conditions. Materials and Methods: Forty-eight multiparous Holstein cows were used in a completely randomized blockdesign trial and randomly assigned to 1 of 3 treatments: control (CTL), without access to shade; access to artificial shade (SH); and shade combined with evaporative cooling (SHplus). Results were analyzed using a mixed procedure
with repeated measures. Results and Discussion: No differences were observed in DMI. Milk yield was not different among treatments, but lactose concentration was greater in SHplus. Treatments CTL and SH had greater BW losses than SHplus. Control cows spent less time grazing. The CTL and SH had higher p.m. rectal temperatures than SHplus, whereas CTL had the highest p.m. respiration rate. Control cows had greater serum insulin levels. Control and SH had
greater BHB and urea concentrations and lower glucose concentration compared with SHplus. The hepatic expression of PCK1, PDK4, and HP genes was downregulated in SH and SHplus relative to control. Hepatic expression of NFKB1 was downregulated, whereas SOCS2 was upregulated, for SHplus compared with CTL.Implications and Applications: Despite the absence of treatment effects on productive variables, changes in blood profiles and hepa... Presentar Todo |
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Palabras claves : |
Energy metabolismhepatic; Gene expression; Heat stress; Late lactation. |
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Thesagro : |
LECHERÍA. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15914/1/Applied-Animal-Science-37p.490503-Martinez.pdf
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Marc : |
LEADER 03020naa a2200289 a 4500
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024 7 $a10.15232/aas.2020-02109$2DOI
100 1 $aMARTÍNEZ, R.
245 $aProviding heat-stress abatement to late-lactation Holstein cows affects hormones, metabolite blood profiles, and hepatic gene expression but not productive responses.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 7 October 2020, Accepted 7 June 2021, Available online 28 July 2021. Corresponding author: rmartine@agro.uba.ar. This article is available under the Creative Commons CC-BY-NC-ND license and permits non-commercial use of the work as published, without adaptation or alteration provided the work is fully attributed.
520 $aABSTRACT: Objective: Our objective was to evaluate the effects of providing shade and shade combined with evaporative cooling on production, cow activity, metabolism, and hepatic gene expression of late-lactation Holstein dairy cows under moderate heat-stress conditions. Materials and Methods: Forty-eight multiparous Holstein cows were used in a completely randomized blockdesign trial and randomly assigned to 1 of 3 treatments: control (CTL), without access to shade; access to artificial shade (SH); and shade combined with evaporative cooling (SHplus). Results were analyzed using a mixed procedure with repeated measures. Results and Discussion: No differences were observed in DMI. Milk yield was not different among treatments, but lactose concentration was greater in SHplus. Treatments CTL and SH had greater BW losses than SHplus. Control cows spent less time grazing. The CTL and SH had higher p.m. rectal temperatures than SHplus, whereas CTL had the highest p.m. respiration rate. Control cows had greater serum insulin levels. Control and SH had greater BHB and urea concentrations and lower glucose concentration compared with SHplus. The hepatic expression of PCK1, PDK4, and HP genes was downregulated in SH and SHplus relative to control. Hepatic expression of NFKB1 was downregulated, whereas SOCS2 was upregulated, for SHplus compared with CTL.Implications and Applications: Despite the absence of treatment effects on productive variables, changes in blood profiles and hepatic expression of target genes were observed among treatments. These results suggest that the provision of shade combined with evaporative cooling was effective in alleviating the negative effects of heat stress.
650 $aLECHERÍA
653 $aEnergy metabolismhepatic
653 $aGene expression
653 $aHeat stress
653 $aLate lactation
700 1 $aPALLADINO, D.A.
700 1 $aBANCHERO, G.
700 1 $aFERNÁNDEZ-MARTIN, R.
700 1 $aNANNI, M.
700 1 $aJULIANO, N.
700 1 $aLORIO, J.
700 1 $aLA MANNA, A.
773 $tApplied Animal Science Volume 37, Issue 4, August 2021, Pages 490-503. OPEN ACCESS. Doi: https://doi.org/10.15232/aas.2020-02109
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó; INIA Treinta y Tres. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
06/06/2022 |
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Autor : |
KOHLI, M.M.; FRANCIS, M. (Ed.). |
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Afiliación : |
MAN MOHAN KOHLI; MARTHA FRANCIS. |
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Título : |
Application of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998. |
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Fecha de publicación : |
2000 |
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Fuente / Imprenta : |
Montevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo, 2000. |
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Páginas : |
175 p. |
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ISBN : |
9974-7586-1-0 |
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Idioma : |
Español
Inglés |
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Contenido : |
The application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies. MenosThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these lat... Presentar Todo |
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Palabras claves : |
BIOTECHNOLOGY; GENETIC TRANSFORMATION; MOLECULAR MARKERS; PLANT BREEDING; RESEARCH PROYECTS; TRITICUM AESTIVUM. |
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Thesagro : |
ARGENTINA; BIOTECNOLOGIA; BRASIL; FITOMEJORAMIENTO; MARCADORES MOLECULARES; MEXICO; PROYECTOS DE INVESTIGACION; SUD AMERICA; TRANSFORMACION GENETICA; TRIGO; TRITICUM AESTIVUM; URUGUAY; USA. |
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Asunto categoría : |
--
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5843/1/RBI-5022-Application.pdf
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Marc : |
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020 $a9974-7586-1-0
100 1 $aKOHLI, M.M.
245 $aApplication of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998.
260 $aMontevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo$c2000
300 $a175 p.
520 $aThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies.
650 $aARGENTINA
650 $aBIOTECNOLOGIA
650 $aBRASIL
650 $aFITOMEJORAMIENTO
650 $aMARCADORES MOLECULARES
650 $aMEXICO
650 $aPROYECTOS DE INVESTIGACION
650 $aSUD AMERICA
650 $aTRANSFORMACION GENETICA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
650 $aURUGUAY
650 $aUSA
653 $aBIOTECHNOLOGY
653 $aGENETIC TRANSFORMATION
653 $aMOLECULAR MARKERS
653 $aPLANT BREEDING
653 $aRESEARCH PROYECTS
653 $aTRITICUM AESTIVUM
700 1 $aFRANCIS, M.
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