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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
07/07/2021 |
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Actualizado : |
27/06/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. |
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Afiliación : |
NATALIA MANISSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; JUAN HERNÁN, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; MARIANA COSSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA E. FRANCIA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Facultad de Medicina, Instituto de Higiene, Universidad de La República, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; ANDRÉS IRIARTE, Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de La República Facultad de Medicina, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Pós-graduação em Ciência Animal nos Trópicos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; SUSANA GONZÁLEZ, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay. |
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Título : |
Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w |
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DOI : |
10.1007/s11033-021-06492-w |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). |
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Contenido : |
Abstract:
Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. |
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Palabras claves : |
Hammondia spp; NEOSPOROSIS; Neotropical canids; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Real-time PCR; TOXOPLASMOSIS. |
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Asunto categoría : |
E16 Enfermedades de los animales |
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Marc : |
LEADER 02829naa a2200349 a 4500
001 1062284
005 2022-06-27
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-021-06492-w$2DOI
100 1 $aMANISSE, N.
245 $aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes$ba contribution to their molecular epidemiology.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026).
520 $aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.
653 $aHammondia spp
653 $aNEOSPOROSIS
653 $aNeotropical canids
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aReal-time PCR
653 $aTOXOPLASMOSIS
700 1 $aCABRERA, A.
700 1 $aHERNÁN, J.
700 1 $aCOSSE, M.
700 1 $aGIANNITTI, F.
700 1 $aFRANCIA, M.E.
700 1 $aGONZÁLEZ, T.
700 1 $aIRIARTE, A.
700 1 $aRIET-CORREA, F.
700 1 $aROBELLO, C.
700 1 $aGONZÁLEZ, S.
773 $tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w
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| 142. |  | SARAVIA, A.; MIRABALLES, C.; RIET-CORREA, F.; CASTRO-JANER, E. Eimeria spp. in dairy calves in Uruguay. Identification, dynamics of oocyst excretion and association with the age of calves. Veterinary Parasitology: Regional Studies and Reports, 25 July 2021, Volume 25, Article number 100588. Doi: https://doi.org/10.1016/j.vprsr.2021.100588. Article history: Received 12 February 2020, Revised 19 February 2021, Accepted 12 May 2021, Available online 14 May 2021.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 145. | | SCHILD, C.; BOABAID F.M.; OLIVERA L.G.S.; MACHADO, M.; VILDOZA, A.; SARAVIA, A.; CUSTODIO A.; COMMAND, C.; MARTÍNEZ, A.; JAURENA, M.; DIXON, R.; RIET-CORREA, F. Osteomalacia as a result of phosphorus deficiency in beef cattle grazing subtropical native pastures in Uruguay. Journal of Veterinary Diagnostic Investigation, 2021. OPEN ACCESS. Doi: https://doi.org/10.1177/10406387211025828| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
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| 147. | | DONCEL, B.; PUENTES, J.D.; CAFFARENA, D.; RIET-CORREA, F.; COSTA, R.A. DA; GIANNITTI, F. Hypomagnesemia in beef cattle.[Hipomagnesemia em bovinos de corte.] Pesquisa Veterinária Brasileira, vol 41:e06826, 2021. Doi:https://doi.org/10.1590/1678-5150-pvb-6826 Article history: Received on December 7, 2020./Accepted for publication on December 21, 2020.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 148. | | MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 157. | | RABAZA, A.; GIANNITTI, F.; FRAGA, M.; MACÍAS-RIOSECO, M.; CORBELLINI, L.G.; RIET-CORREA, F.; HIRIGOYEN, D.; TURNER, K.M.E.; EISLER, ,M.C. Serological evidence of human infection with Coxiella burnetii after occupational exposure to aborting cattle. Veterinary Sciences, 2021, Volume 8, Issue 9, Article Number196. Doi: https://doi.org/10.3390/vetsci8090196 Article history: Received: 22 July 2021/Accepted: 10 September 2021/Published: 16 September 2021.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 159. | | GARZÓN, J. P.; SILVEIRA, C.S.; URIOSTE, V.; STIRLING, S.; LOCKHART, B.; FARIÑA, S.; GIANNITTI, F.; GASTAL, G.D.A. Pérdidas embrionarias y fetales en un rodeo de bovinos holstein de uruguay: efectos de la paridad, el biotipo y la exposición a Neospora caninum y el virus de la leucosis bovina. In: Jornadas Uruguayas de Buiatría, 48., 2021. Paysandú, Uruguay: Centro Médico Veterinario de Paysandú; Filial de la Sociedad de Medicina Veterinaria del Uruguaya; Sociedad Uruguaya de Buiatría, 31 de Mayo al 4 de Junio 2021. [Poster]. p.260-263.| Biblioteca(s): INIA La Estanzuela. |
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| 160. | | DORSCH, M.; CASAUX, M.L.; CALLEROS, L.; ARÁOZ, V.; CAFFARENA, D.; MONESIGLIO, M.C.; BARCELLOS, M; SILVEIRA, C.S.; PERDOMO, T.; BANCHERO, G.; UZAL, F.A.; FRAGA, M.; GIANNITTI, F. Placentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay. Revista Argentina de Microbiología, 2022, volume 54, issue 1, pages 25-30. Open Acces. Doi:https://doi.org/10.1016/j.ram.2021.02.005 Article history: Received 22 July 2020/ Accepted 28 February 2021. Corresponding author:E-mail address: fgiannitti@inia.org.uy (F. Giannitti).
This work was financially supported by grants N-15156 PL 150 00 and PL 27 N-23398 of the...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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