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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/03/2021 |
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Actualizado : |
14/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NASCIMENTO, D. C.; DINI, M.; CARPENEDO, S.; RASEIRA, M. DO C. B. |
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Afiliación : |
D. C. NASCIMENTO, Postgraduate Program in Agronomy (PPGA), Faculty of Agronomy "Eliseu Maciel", Federal University of Pelotas (FAEM-UFPel), Pelotas (RS), Brazil; MAXIMILIANO ANTONIO DINI VIÑOLY, Postgraduate Program in Agronomy (PPGA), Faculty of Agronomy "Eliseu Maciel", Federal University of Pelotas (FAEM-UFPel), Pelotas (RS), Brazil; S. CARPENEDO, Laboratory of Fruit Breeding, Embrapa Temperate Agriculture Pelotas (RS), Brazil; M. C. B. RASEIRA, Laboratory of Fruit Breeding, Embrapa Temperate Agriculture Pelotas (RS), Brazil. |
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Título : |
Germination and development of 'Precocinho' peach embryos: asepsis and use of PPM tm in culture medium. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Journal of Experimental Agriculture International, 2018, v. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065 |
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ISSN : |
2457-0591 |
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DOI : |
10.9734/JEAI/2018/41065 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018.
Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Republic of Serbia.
Reviewers: (1) Zeynel Dalkiliç, College of Agriculture, Adnan Menderes University, Turkey. (2) Nebi Bilir, Suleyman Demirel University, Turkey. (3) Martin Potgieter, University of Limpopo, South Africa. Complete Peer review History: http://www.sciencedomain.org/review-history/24416 |
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Contenido : |
ABSTRACT.
Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos. |
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Palabras claves : |
Disinfestation; Embryo culture; Immature embryos. |
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Thesagro : |
PRUNUS PERSICA (L.). |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15317/1/Nascimento-et-al.-2018.-Germination-and-Development-of-Precocinho-Peach-JEAI.pdf
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Marc : |
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024 7 $a10.9734/JEAI/2018/41065$2DOI
100 1 $aNASCIMENTO, D. C.
245 $aGermination and development of 'Precocinho' peach embryos$basepsis and use of PPM tm in culture medium.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 19 February 2018; Accepted 27 April 2018; Published 2 May 2018. Article information: Editor(s): (1) Biljana Bojovic, Assistant Professor, Faculty of Science, Institute of Biology and Ecology, University of Kragujevac, Republic of Serbia. Reviewers: (1) Zeynel Dalkiliç, College of Agriculture, Adnan Menderes University, Turkey. (2) Nebi Bilir, Suleyman Demirel University, Turkey. (3) Martin Potgieter, University of Limpopo, South Africa. Complete Peer review History: http://www.sciencedomain.org/review-history/24416
520 $aABSTRACT. Embryo culture is a technique used to rescue immature embryos which would not germinate under natural conditions. However, one of the biggest problems of this process is the contamination by microorganisms. The objective of this work was to test asepsis methods and the use of PPMTM (Plant Preservative MixtureTM) in the culture medium for the germination and development of 'Precocinho' peach embryos. After extraction from the fruits, seeds were submitted to treatments with thimerosal, sodium hypochlorite (NaClO), flame sterilization and moxifloxacin hydrochloride, in culture medium with and without addition of PPMTM, totaling nine treatments. The experimental design was completely randomized, with 20 replicates per treatment. Three evaluations were performed: in vitro establishment (at 55 days of cultivation), embryo development (at 65 days of cultivation) and acclimatization (20 days after transplanting in the greenhouse). Six treatments did not had any contamination, on the first evaluation. However, 65 days after culture, the NaClO with PPMTM addition presented the best results for the variables analyzed. The average seedling survival was 81.6%, after acclimatization. The use of sodium hypochlorite with the addition of PPMTM is shown as the most efficient treatment, among the tested ones, for the germination and development of 'Precocinho' peach embryos.
650 $aPRUNUS PERSICA (L.)
653 $aDisinfestation
653 $aEmbryo culture
653 $aImmature embryos
700 1 $aDINI, M.
700 1 $aCARPENEDO, S.
700 1 $aRASEIRA, M. DO C. B.
773 $tJournal of Experimental Agriculture International, 2018$gv. 22, n. 6, p. 1-7. Article no.JEAI.41065. Doi: https://doi.org/10.9734/JEAI/2018/41065
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
21/02/2014 |
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Actualizado : |
08/06/2018 |
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Tipo de producción científica : |
Documentos |
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Autor : |
MÉNDEZ, R.; ROEL, A. |
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Afiliación : |
RAMÓN FELIPE MÉNDEZ LARROSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ALVARO ROEL DELLAZOPPA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Bioclimático de variedades y líneas experimentales. |
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Complemento del título : |
Ecofisiología del cultivo. II. Modelos de simulación. |
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Fecha de publicación : |
2003 |
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Fuente / Imprenta : |
ln: INIA TREINTA Y TRES. PROGRAMA NACIONAL DE ARROZ. Investigaciones agronómicas: reporte técnico anual 2003. Área cultivos. Treinta y Tres (Uruguay): INIA, 2003. |
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Páginas : |
cap. 2, p. 9-16. |
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Serie : |
(INIA Reporte Técnico Anual; 01) |
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Idioma : |
Español |
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Thesagro : |
ARROZ; CLIMATOLOGIA; MODELOS DE SIMULACION; VARIEDADES. |
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Asunto categoría : |
P40 Meteorología y climatología |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/10099/1/RTA-2003-cap-2-p.9-16.pdf
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Marc : |
LEADER 00647naa a2200193 a 4500
001 1031115
005 2018-06-08
008 2003 bl uuuu u00u1 u #d
100 1 $aMÉNDEZ, R.
245 $aBioclimático de variedades y líneas experimentales.
260 $c2003
300 $acap. 2, p. 9-16.
490 $a(INIA Reporte Técnico Anual; 01)
650 $aARROZ
650 $aCLIMATOLOGIA
650 $aMODELOS DE SIMULACION
650 $aVARIEDADES
700 1 $aROEL, A.
773 $tln: INIA TREINTA Y TRES. PROGRAMA NACIONAL DE ARROZ. Investigaciones agronómicas: reporte técnico anual 2003. Área cultivos. Treinta y Tres (Uruguay): INIA, 2003.
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INIA Treinta y Tres (TT) |
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