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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
01/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
QUEZADA, M.; PASTINA, M.M.; RAVEST, G.; SILVA, P.; VIGNALE, B.; CABRERA, D.; HINRICHSEN, P.; GARCÍA, A.A.F.; PRITSCH, C. |
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Afiliación : |
CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
A first genetic map of Acca sellowiana based on ISSR, AFLP and SSR markers. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Scientia Horticulturae, 2014, v.169, no.2, p. 138-146. |
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ISSN : |
0304-4238 |
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DOI : |
10.1016/j.scienta.2014.02.009 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 13 May 2013 / Received in revised form 24 January 2014 / Accepted 12 February 2014 / Available online 14 March 2014. |
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Contenido : |
ABSTRACT.
A first integrated linkage map of Acca sellowiana (Berg.) Burret (2. n= 2. x= 22), commonly known as 'pineapple guava' was constructed using a 160 (TCO. ×. BR) full-sib F1 progeny based on intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. To develop an integrated map, we used a novel statistical approach that provided a simultaneous estimation of linkage and linkage phases based on maximum-likelihood analysis. The integrated map comprised 219 (27 ISSRs and 192 AFLPs) linked markers that were assigned to 10 major linkage groups (LG) representing a total map length of 2875. cM and average distance between markers of 13.8. cM. TCO (14 LG, 83 markers, 1264.6. cM) and BR (18 LG, 94 markers, 1295.1. cM) parent maps were also constructed through the same statistical approach but using only appropriate pseudo-testcross configuration markers. All the LG of the integrated map shared markers with either TCO or, BR or both parental maps. The integrated map provides a framework for further genetic and genomic analyses in A. sellowiana.
© 2014 Elsevier B.V. |
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Palabras claves : |
Feijoa sellowiana; Linkage map; Multipoint estimates; Orphan species; Two-way pseudo-testcross. |
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Thesagro : |
ACCA SELLOWIANA; FITOMEJORAMIENTO; GUAYABO DEL PAÍS; MAPAS GENÉTICOS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02290naa a2200361 a 4500
001 1050792
005 2019-10-09
008 2014 bl uuuu u00u1 u #d
022 $a0304-4238
024 7 $a10.1016/j.scienta.2014.02.009$2DOI
100 1 $aQUEZADA, M.
245 $aA first genetic map of Acca sellowiana based on ISSR, AFLP and SSR markers.$h[electronic resource]
260 $c2014
500 $aArticle history: Received 13 May 2013 / Received in revised form 24 January 2014 / Accepted 12 February 2014 / Available online 14 March 2014.
520 $aABSTRACT. A first integrated linkage map of Acca sellowiana (Berg.) Burret (2. n= 2. x= 22), commonly known as 'pineapple guava' was constructed using a 160 (TCO. ×. BR) full-sib F1 progeny based on intersimple sequence repeat (ISSR), amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers. To develop an integrated map, we used a novel statistical approach that provided a simultaneous estimation of linkage and linkage phases based on maximum-likelihood analysis. The integrated map comprised 219 (27 ISSRs and 192 AFLPs) linked markers that were assigned to 10 major linkage groups (LG) representing a total map length of 2875. cM and average distance between markers of 13.8. cM. TCO (14 LG, 83 markers, 1264.6. cM) and BR (18 LG, 94 markers, 1295.1. cM) parent maps were also constructed through the same statistical approach but using only appropriate pseudo-testcross configuration markers. All the LG of the integrated map shared markers with either TCO or, BR or both parental maps. The integrated map provides a framework for further genetic and genomic analyses in A. sellowiana. © 2014 Elsevier B.V.
650 $aACCA SELLOWIANA
650 $aFITOMEJORAMIENTO
650 $aGUAYABO DEL PAÍS
650 $aMAPAS GENÉTICOS
653 $aFeijoa sellowiana
653 $aLinkage map
653 $aMultipoint estimates
653 $aOrphan species
653 $aTwo-way pseudo-testcross
700 1 $aPASTINA, M.M.
700 1 $aRAVEST, G.
700 1 $aSILVA, P.
700 1 $aVIGNALE, B.
700 1 $aCABRERA, D.
700 1 $aHINRICHSEN, P.
700 1 $aGARCÍA, A.A.F.
700 1 $aPRITSCH, C.
773 $tScientia Horticulturae, 2014$gv.169, no.2, p. 138-146.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/01/2020 |
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Actualizado : |
22/01/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PAZ, L.N.; HAMOND, C.; DIAS, C.S.; CURVELO, V.P.; MEDEIROS, M.A.; ORIÁ, A.P.; PINNA, M.H. |
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Afiliación : |
LUCAS NOGUEIRA PAZ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; CAMILA HAMOND, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLA SILVA DIAS, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; VITOR P. CURVELO, Getúlio Vargas Zoobotanic Park, Salvador, BA, Brazil; MARCO ALBERTO MEDEIROS, Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Ministry of Health, FIOCRUZ, Brazil; ARIANNE PONTES ORIÁ, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil; MELISSA HANZEN PINNA, School of Veterinary Medicine and Zootechny, Federal University of Bahia, Salvador, BA, Brazil. |
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Título : |
Detection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution] |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
EcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0 |
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ISSN : |
1612-9202 |
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DOI : |
10.1007/s10393-019-01452-0 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019.
The online version of this article contains supplementary material, which is available to authorized users. |
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Contenido : |
ABSTRACT.
Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers ≥ 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians.
© 2019, EcoHealth Alliance. |
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Palabras claves : |
Crocodilian; Diagnosis; PLATAFORMA SALUD ANIMAL. |
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Thesagro : |
LEPTOSPIROSIS; REPTILES; ZOONOSIS. |
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Asunto categoría : |
A50 Investigación agraria |
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Marc : |
LEADER 02457naa a2200301 a 4500
001 1060670
005 2020-01-22
008 2019 bl uuuu u00u1 u #d
022 $a1612-9202
024 7 $a10.1007/s10393-019-01452-0$2DOI
100 1 $aPAZ, L.N.
245 $aDetection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution]$h[electronic resource]
260 $c2019
500 $aArticle history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019. The online version of this article contains supplementary material, which is available to authorized users.
520 $aABSTRACT. Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers ≥ 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians. © 2019, EcoHealth Alliance.
650 $aLEPTOSPIROSIS
650 $aREPTILES
650 $aZOONOSIS
653 $aCrocodilian
653 $aDiagnosis
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aHAMOND, C.
700 1 $aDIAS, C.S.
700 1 $aCURVELO, V.P.
700 1 $aMEDEIROS, M.A.
700 1 $aORIÁ, A.P.
700 1 $aPINNA, M.H.
773 $tEcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0
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