02457naa a2200301 a 450000100080000000500110000800800410001902200140006002400360007410000140011024501340012426000090025850002210026752013670048865000180185565000130187365000130188665300160189965300140191565300280192970000150195770000150197270000180198770000190200570000160202470000160204077300990205610606702020-01-22       2019    bl uuuu     u00u1 u    #d  a1612-92027 a10.1007/s10393-019-01452-02DOI1 aPAZ, L.N.  aDetection of Leptospira spp. in Captive Broad-Snouted Caiman (Caiman latirostris). [Original Contribution]h[electronic resource]  c2019  aArticle history: Received 12 November 2018 / Accepted 30 September 2019 / Published Online 07 November 2019. The online version of this article contains supplementary material, which is available to authorized users.  aABSTRACT. Leptospira sp. is an important waterborne zoonotic bacterium, known to cause infection in animals and humans worldwide. The role of reptiles in the transmission of this microorganism is poorly understood and historically neglected. This study aimed to investigate the presence of anti-Leptospira spp. antibodies and leptospiral DNA in captive Caiman latirostris (broad-snouted caiman). Of the 23 reptiles studied by microscopic agglutination test (MAT), 22/23 (95.65%) were considered reactive (titers &#8805; 100) and 1/23 (4.35%) non-reactive (titer < 100). The serogroup with highest occurrence was Grippotyphosa (68.18%, n = 15/22) followed by serogroup Djasiman (18.18%, n = 4/22). Specific amplification of Leptospira spp. gene lipL32 was observed in six (26.09%, n = 6/23) blood samples. Five of six samples, previously detected as pathogenic leptospira by PCR, were amplified and sequenced. All the samples corresponded to the pathogenic species Leptospira interrogans (presented 100% of identity) using the PCR targeting to secY gene. We demonstrated high detection of DNA of L. interrogans in crocodilians, and the authors suggest that further research is needed to elucidate the impact of Leptospira spp. infection in health broad-snouted caimans as well as the pathophysiology of leptospirosis in crocodilians.  © 2019, EcoHealth Alliance.  aLEPTOSPIROSIS  aREPTILES  aZOONOSIS  aCrocodilian  aDiagnosis  aPLATAFORMA SALUD ANIMAL1 aHAMOND, C.1 aDIAS, C.S.1 aCURVELO, V.P.1 aMEDEIROS, M.A.1 aORIÁ, A.P.1 aPINNA, M.H.  tEcoHealth, 1 December 2019, Volume 16, Issue 4, Pages 694-700. Doi: 10.1007/s10393-019-01452-0