|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
11/09/2014 |
Actualizado : |
15/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
NARANCIO, R.; ZORRILLA, P.; ROBELLO, C.; GONZÁLEZ-ARCOS, M.; VILARÓ, F.; PRITSCH, C.; DALLA RIZZA, M. |
Afiliación : |
RAFAEL NARANCIO FERES, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MATIAS GONZALEZ ARCOS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FRANCISCO LUIS VILARO PAREJA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARCO DALLA RIZZA VILARO, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
Título : |
Insights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum. |
Fecha de publicación : |
2013 |
Fuente / Imprenta : |
European Journal of Plant Pathology, 2013, v.136, no.4, p.823-835. |
ISSN : |
0929-1873 |
DOI : |
10.1007/s10658-013-0210-y |
Idioma : |
Inglés |
Notas : |
Article history: Accepted: 27 March 2013 / Published online: 28 April 2013. |
Contenido : |
ABSTRACT.
Solanum commersonii is a wild species related to the cultivated potato. Some S. commersonii genotypes have been proven to be resistant to the pathogenic bacteria Ralstonia solanacearum, which causes damage in potato and other economically important crops. Here an expression analysis of the response of a resistant S. commersonii genotype against R. solanacearum was performed using microarrays. The aims of this work were to elucidate the molecular processes involved in the interaction, establish the timing of the response, and contribute to identify genes related to the resistance. The response to the treatment was already initiated at 6 h post-inoculation (hpi) and was established at 24 hpi; during this period, a high number of genes was differentially expressed and several candidate genes for the resistance of S. commersonii to R. solanacearum were identified. At an early stage, the photosynthetic process was highly repressed and several genes encoding proteins related to reactive oxygen species (ROS) production were differentially expressed. The induction of ERF and ACC-oxidase genes related to the ethylene pathway and PR1 related to the salicylic acid pathway suggested the induction of both pathways, and back up the previously reported hemibiotrophic nature of the pathogen. Five genes related to plant defence and observed to be differentially expressed at the first two time points were validated by real time PCR. This work gives a glimpse to the molecular processes involved in S. commersonii resistance and identifies the species as a valuable genetic source for potato breeding against bacterial wilt.
© 2013 KNPV. MenosABSTRACT.
Solanum commersonii is a wild species related to the cultivated potato. Some S. commersonii genotypes have been proven to be resistant to the pathogenic bacteria Ralstonia solanacearum, which causes damage in potato and other economically important crops. Here an expression analysis of the response of a resistant S. commersonii genotype against R. solanacearum was performed using microarrays. The aims of this work were to elucidate the molecular processes involved in the interaction, establish the timing of the response, and contribute to identify genes related to the resistance. The response to the treatment was already initiated at 6 h post-inoculation (hpi) and was established at 24 hpi; during this period, a high number of genes was differentially expressed and several candidate genes for the resistance of S. commersonii to R. solanacearum were identified. At an early stage, the photosynthetic process was highly repressed and several genes encoding proteins related to reactive oxygen species (ROS) production were differentially expressed. The induction of ERF and ACC-oxidase genes related to the ethylene pathway and PR1 related to the salicylic acid pathway suggested the induction of both pathways, and back up the previously reported hemibiotrophic nature of the pathogen. Five genes related to plant defence and observed to be differentially expressed at the first two time points were validated by real time PCR. This work gives a glimpse to the molecular proce... Presentar Todo |
Thesagro : |
BACTERIOSIS; CULTIVO; FENOTIPOS; FITOMEJORAMIENTO; PAPA; RESISTENCIA A ENFERMEDADES. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02622naa a2200301 a 4500 001 1050149 005 2019-10-15 008 2013 bl uuuu u00u1 u #d 022 $a0929-1873 024 7 $a10.1007/s10658-013-0210-y$2DOI 100 1 $aNARANCIO, R. 245 $aInsights on gene expression response of a characterized resistant genotype of Solanum commersonii Dun. against Ralstonia solanacearum.$h[electronic resource] 260 $c2013 500 $aArticle history: Accepted: 27 March 2013 / Published online: 28 April 2013. 520 $aABSTRACT. Solanum commersonii is a wild species related to the cultivated potato. Some S. commersonii genotypes have been proven to be resistant to the pathogenic bacteria Ralstonia solanacearum, which causes damage in potato and other economically important crops. Here an expression analysis of the response of a resistant S. commersonii genotype against R. solanacearum was performed using microarrays. The aims of this work were to elucidate the molecular processes involved in the interaction, establish the timing of the response, and contribute to identify genes related to the resistance. The response to the treatment was already initiated at 6 h post-inoculation (hpi) and was established at 24 hpi; during this period, a high number of genes was differentially expressed and several candidate genes for the resistance of S. commersonii to R. solanacearum were identified. At an early stage, the photosynthetic process was highly repressed and several genes encoding proteins related to reactive oxygen species (ROS) production were differentially expressed. The induction of ERF and ACC-oxidase genes related to the ethylene pathway and PR1 related to the salicylic acid pathway suggested the induction of both pathways, and back up the previously reported hemibiotrophic nature of the pathogen. Five genes related to plant defence and observed to be differentially expressed at the first two time points were validated by real time PCR. This work gives a glimpse to the molecular processes involved in S. commersonii resistance and identifies the species as a valuable genetic source for potato breeding against bacterial wilt. © 2013 KNPV. 650 $aBACTERIOSIS 650 $aCULTIVO 650 $aFENOTIPOS 650 $aFITOMEJORAMIENTO 650 $aPAPA 650 $aRESISTENCIA A ENFERMEDADES 700 1 $aZORRILLA, P. 700 1 $aROBELLO, C. 700 1 $aGONZÁLEZ-ARCOS, M. 700 1 $aVILARÓ, F. 700 1 $aPRITSCH, C. 700 1 $aDALLA RIZZA, M. 773 $tEuropean Journal of Plant Pathology, 2013$gv.136, no.4, p.823-835.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela; INIA Tacuarembó; INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
06/06/2022 |
Autor : |
KOHLI, M.M.; FRANCIS, M. (Ed.). |
Afiliación : |
MAN MOHAN KOHLI; MARTHA FRANCIS. |
Título : |
Application of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998. |
Fecha de publicación : |
2000 |
Fuente / Imprenta : |
Montevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo, 2000. |
Páginas : |
175 p. |
ISBN : |
9974-7586-1-0 |
Idioma : |
Español Inglés |
Contenido : |
The application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies. MenosThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these lat... Presentar Todo |
Palabras claves : |
BIOTECHNOLOGY; GENETIC TRANSFORMATION; MOLECULAR MARKERS; PLANT BREEDING; RESEARCH PROYECTS; TRITICUM AESTIVUM. |
Thesagro : |
ARGENTINA; BIOTECNOLOGIA; BRASIL; FITOMEJORAMIENTO; MARCADORES MOLECULARES; MEXICO; PROYECTOS DE INVESTIGACION; SUD AMERICA; TRANSFORMACION GENETICA; TRIGO; TRITICUM AESTIVUM; URUGUAY; USA. |
Asunto categoría : |
-- F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5843/1/RBI-5022-Application.pdf
|
Marc : |
LEADER 02905nam a2200373 a 4500 001 1055271 005 2022-06-06 008 2000 bl uuuu u00u1 u #d 020 $a9974-7586-1-0 100 1 $aKOHLI, M.M. 245 $aApplication of biotechnologies to wheat breeding. Proceedings of a conference at La Estanzuela, Uruguay. November 19-20, 1998. 260 $aMontevideo (Uruguay): Instituto Nacional de Investigación Agropecuaria; Centro Internacional de Mejoramiento de Maíz y Trigo$c2000 300 $a175 p. 520 $aThe application of biotechn'ology to crop improvement has been the focus of much research around the world. Tissue culture and selection of somaclonal variation were developed many years ago, followed more recently by molecular markers and genetic engineering. This meeting focused on providing updates on the actual successes to date on the application of several areas of biotechnology to wheat improvement. What is clear is that there continues to be optimism regarding the potential for biotechnology to be applied to wheat improvement. It is also noteworthy that as was predicted by many scientists, many approaches are proving more difficult in wheat than in other crops. Molecular markers, for example, are difficult to apply routinely given the large genome, hexaploid genetics, and lower levels of polymorphisms. New marker systems such as AFLPs and microsatellites are an improvement over previous RFLPs, but still require significant investments to achieve similar results possible in other, more amenable species. What is encouraging are the successes to identify the genetics of complex traits in wheat such as quality, Fusarium and rust resistance. Some of these will surely provide useful applications in wheat improvement and all mapping efforts provide critical genetic information for further studies at the gene level. These will be increasingly important as genomic approaches (gene expression, proteomics, etc.) become available and are applied to wheat. In many ways, these latest genomics approaches should be more 'genome independent' and thus readily applicable to large, complex genome species such as wheat. Coupled with the relatively easy ability to produce doubled haploids, wheat may prove to be an extremely important species for genomic studies. 650 $aARGENTINA 650 $aBIOTECNOLOGIA 650 $aBRASIL 650 $aFITOMEJORAMIENTO 650 $aMARCADORES MOLECULARES 650 $aMEXICO 650 $aPROYECTOS DE INVESTIGACION 650 $aSUD AMERICA 650 $aTRANSFORMACION GENETICA 650 $aTRIGO 650 $aTRITICUM AESTIVUM 650 $aURUGUAY 650 $aUSA 653 $aBIOTECHNOLOGY 653 $aGENETIC TRANSFORMATION 653 $aMOLECULAR MARKERS 653 $aPLANT BREEDING 653 $aRESEARCH PROYECTS 653 $aTRITICUM AESTIVUM 700 1 $aFRANCIS, M.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|