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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
09/09/2014 |
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Actualizado : |
25/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROSAS, J.; BONNECARRERE, M.; PÉREZ DE VIDA, F. |
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Afiliación : |
JUAN EDUARDO ROSAS CAISSIOLS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.). |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2014, v. 17, no. 2, p.95-101 |
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ISSN : |
0717-3458 |
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DOI : |
10.1016/j.ejbt.2014.02.003 |
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Idioma : |
Inglés |
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Notas : |
Article history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014. |
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Contenido : |
Abstract
Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice. |
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Palabras claves : |
CLEARFIELD RICE; DNA - BASED RESISTANCE DIAGNOSIS; HERBICIDE RESISTANCE; KASP; RED RICE; SNP. |
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Thesagro : |
ARROZ ROJO; IMIDAZOLINONAS; RESISTENCIA A HERBICIDAS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02123naa a2200289 a 4500
001 1050077
005 2018-09-25
008 2014 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.1016/j.ejbt.2014.02.003$2DOI
100 1 $aROSAS, J.
245 $aOne-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.).$h[electronic resource]
260 $c2014
500 $aArticle history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014.
520 $aAbstract Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice.
650 $aARROZ ROJO
650 $aIMIDAZOLINONAS
650 $aRESISTENCIA A HERBICIDAS
653 $aCLEARFIELD RICE
653 $aDNA - BASED RESISTANCE DIAGNOSIS
653 $aHERBICIDE RESISTANCE
653 $aKASP
653 $aRED RICE
653 $aSNP
700 1 $aBONNECARRERE, M.
700 1 $aPÉREZ DE VIDA, F.
773 $tElectronic Journal of Biotechnology, 2014$gv. 17, no. 2, p.95-101
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