02123naa a2200289 a 450000100080000000500110000800800410001902200140006002400360007410000140011024501290012426000090025350001050026252011770036765000150154465000190155965000290157865300200160765300370162765300250166465300090168965300130169865300080171170000210171970000230174077300700176310500772018-09-25       2014    bl uuuu     u00u1 u    #d  a0717-34587 a10.1016/j.ejbt.2014.02.0032DOI1 aROSAS, J.  aOne-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.).h[electronic resource]  c2014  aArticle history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014.  aAbstract Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice.  aARROZ ROJO  aIMIDAZOLINONAS  aRESISTENCIA A HERBICIDAS  aCLEARFIELD RICE  aDNA - BASED RESISTANCE DIAGNOSIS  aHERBICIDE RESISTANCE  aKASP  aRED RICE  aSNP1 aBONNECARRERE, M.1 aPÉREZ DE VIDA, F.  tElectronic Journal of Biotechnology, 2014gv. 17, no. 2, p.95-101