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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
24/05/2022 |
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Actualizado : |
24/05/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
RAFFO, M.A.; AZZIMONTI, G.; PEREYRA, S.; PRITSCH, C.; LADO, B.; DREISIGACKER, S.; QUINCKE, M.; CASTRO, A.; SILVA, P.; GARCIA, R.; PEREIRA, F.; GERMAN, S. |
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Afiliación : |
MIGUEL ANGEL RAFFO BUSCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GUSTAVO AZZIMONTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ANTONIA PEREYRA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CLARA PRITSCH, Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; BETTINA LADO, Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, DF, Mexico.; MARTIN CONRADO QUINCKE WALDEN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Facultad de Agronomía, Universidad de la República, Garzón 780, CP 12900 Montevideo, Uruguay.; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICHARD ANSELMO GARCIA USUCA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO PEREIRA CALISTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Introgression of the coupled Fhb1-Sr2 to increase Fusarium head blight and stem rust resistance of elite wheat cultivars. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Plant Genetic Resources: Characterization and Utilization, 1-10, 2022. Doi: https://doi.org/10.1017/S1479262122000107 |
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DOI : |
10.1017/S1479262122000107 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 1 June 2021/Revised: 20 April 2022/Accepted: 20 April 2022. Author for correspondence: S. Germán, E-mail: sgerman@inia.org.uy. The supplementary material for this article can be found at https://doi.org/10.1017/S1479262122000107 |
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Contenido : |
Abstract:
Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ?Génesis 2375?. The most resistant lines to both diseases are expected to be valuable genetic resources in breeding for durable resistance to FHB and SR. MenosAbstract:
Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ... Presentar Todo |
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Palabras claves : |
FUSARIUM HEAD BLIGHT; STEM RUST; TRITICUM AESTIVUM; UMN10. |
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Thesagro : |
TRIGO. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02904naa a2200337 a 4500
001 1063162
005 2022-05-24
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1017/S1479262122000107$2DOI
100 1 $aRAFFO, M.A.
245 $aIntrogression of the coupled Fhb1-Sr2 to increase Fusarium head blight and stem rust resistance of elite wheat cultivars.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 1 June 2021/Revised: 20 April 2022/Accepted: 20 April 2022. Author for correspondence: S. Germán, E-mail: sgerman@inia.org.uy. The supplementary material for this article can be found at https://doi.org/10.1017/S1479262122000107
520 $aAbstract: Fusarium head blight (FHB) and stem rust (SR) threaten the sustainability of wheat production worldwide. Fhb1 and Sr2 confer partial durable resistance to FHB and SR, respectively. Despite resistant alleles of both genes are linked in repulsion, lines with Fhb1-Sr2 in coupling were developed at the University of Minnesota, USA. Marker-assisted backcrossing was used to incorporate the coupled Fhb1-Sr2 into four elite INIA-Uruguay spring wheat varieties lacking both genes and expressing different levels of FHB and SR resistance. In each case, the initial cross between the donor line and recurrent parent was backcrossed three times. Genotypes carrying Fhb1-Sr2 were selected using the molecular marker UMN10. In BC3F3 families, retention of Fhb1-Sr2 was further confirmed with the markers SNP3BS-8 and Sr2-ger9 for Fhb1 and Sr2, respectively. BC3F3 homozygous lines contrasting at UMN10, SNP3BS-8 and Sr2-ger9 were obtained to quantify the effect of Fhb1-Sr2 on the resistance to FHB under controlled conditions and to SR under field conditions. After 26 months period, successful introgression of Fhb1-Sr2 into the four cultivars was achieved, representing novel wheat genetic resources. Lines homozygous for the resistant alleles of Fhb1 were significantly more resistant to FHB as reflected by an 18% reduction of average FHB area under the disease progress curve. A significant effect of Sr2 on SR field resistance was observed in lines derived from the most susceptible cultivar ?Génesis 2375?. The most resistant lines to both diseases are expected to be valuable genetic resources in breeding for durable resistance to FHB and SR.
650 $aTRIGO
653 $aFUSARIUM HEAD BLIGHT
653 $aSTEM RUST
653 $aTRITICUM AESTIVUM
653 $aUMN10
700 1 $aAZZIMONTI, G.
700 1 $aPEREYRA, S.
700 1 $aPRITSCH, C.
700 1 $aLADO, B.
700 1 $aDREISIGACKER, S.
700 1 $aQUINCKE, M.
700 1 $aCASTRO, A.
700 1 $aSILVA, P.
700 1 $aGARCIA, R.
700 1 $aPEREIRA, F.
700 1 $aGERMAN, S.
773 $tPlant Genetic Resources: Characterization and Utilization, 1-10, 2022. Doi: https://doi.org/10.1017/S1479262122000107
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
09/03/2018 |
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Actualizado : |
24/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GARCÍA, J.A.; GARCÍA Y SANTOS, C.; ROSAS, J.E.; DUTRA, F.; GARDNER, D. |
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Afiliación : |
JUAN AGUSTÍN GARCÍA, Centro Universitario Regional Este, Universidad de la República, Uruguay.; CARMEN GARCÍA Y SANTOS, Departamento de Toxicología, Facultad de Veterinaria, Universidad de la República, Uruguay.; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA, DILAVE (División Laboratorios Veterinarios), Regional Este, MGAP, Uruguay.; DALE GARDNER, USDA, ARX, Poisonuous Plant Research Laboratory, USA. |
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Título : |
A survey of Senecio spp. affecting livestock in Uruguay and their associated pyrrolizidine alkaloid content. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Ciência Rural, feb., 2018, v.48(2), p. e20170621. |
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ISSN : |
1678-4596 |
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DOI : |
10.1590/0103-8478cr20170621 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 09.04.17; Approved 11.30.17 Returned by the author 12.27.17. CR-2017-0621.R1 |
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Contenido : |
ABSTRACT:
In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved in the reported outbreaks of seneciosis. MenosABSTRACT:
In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved... Presentar Todo |
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Palabras claves : |
ASTERACEAE; PLANT SECONDARY METABOLITES; PLANTAS TÓXICAS; POISONOUS PLANTS; SENECIOSIS. |
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Thesagro : |
RUMIANTES; SENECIO. |
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Asunto categoría : |
Q53 Contaminación y toxicología de alimentos para animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8891/1/Rosas-Arb-2018-1.pdf
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Marc : |
LEADER 02472naa a2200289 a 4500
001 1058223
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
022 $a1678-4596
024 7 $a10.1590/0103-8478cr20170621$2DOI
100 1 $aGARCÍA, J.A.
245 $aA survey of Senecio spp. affecting livestock in Uruguay and their associated pyrrolizidine alkaloid content.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 09.04.17; Approved 11.30.17 Returned by the author 12.27.17. CR-2017-0621.R1
520 $aABSTRACT: In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved in the reported outbreaks of seneciosis.
650 $aRUMIANTES
650 $aSENECIO
653 $aASTERACEAE
653 $aPLANT SECONDARY METABOLITES
653 $aPLANTAS TÓXICAS
653 $aPOISONOUS PLANTS
653 $aSENECIOSIS
700 1 $aGARCÍA Y SANTOS, C.
700 1 $aROSAS, J.E.
700 1 $aDUTRA, F.
700 1 $aGARDNER, D.
773 $tCiência Rural, feb., 2018$gv.48(2), p. e20170621.
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