| |
|
|
 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha : |
28/03/2022 |
|
Actualizado : |
02/12/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Autor : |
DE ARMAS, S.; GALVÁN, G. A.; LAPAZ, M. I.; GONZÁLEZ-BARRIOS, P.; VICENTE, E.; PIANZZOLA, M. J.; SIRI, M. I. |
|
Afiliación : |
STEFANIE DE ARMAS, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY.; Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; GUILLERMO A. GALVÁN, Departamento de Producción Vegetal, Centro Regional Sur (CRS), Facultad de Agronomía, UDELAR, Canelones, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. LAPAZ, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, uy. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; PABLO GONZÁLEZ-BARRIOS, Departamento de Biometría, Estadística y Computación. Facultad de Agronomía, UDELAR, Montevideo, UY.; CARLOS ESTEBAN VICENTE CASTRO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA J. PIANZZOLA, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA).; MARÍA I. SIRI, Área Microbiología, Departamento de Biociencias, Facultad de Química, UDELAR, Montevideo, UY. Programa de Desarrollo de las Ciencias Básicas (PEDECIBA). |
|
Título : |
Phylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay. |
|
Fecha de publicación : |
2022 |
|
Fuente / Imprenta : |
Plant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE |
|
DOI : |
10.1094/PDIS-06-21-1140-RE |
|
Idioma : |
Inglés |
|
Notas : |
Published Online:27 Mar 2022.
Corresponding author: María I. Siri; Email: msiri@fq.edu.uy. |
|
Contenido : |
Abstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens. MenosAbstract
Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves... Presentar Todo |
|
Palabras claves : |
ALLIUM CEPA; CENTER ROT; PANTOEA SPP; PATHOGEN DIVERSITY. |
|
Asunto categoría : |
-- |
|
Marc : |
LEADER 03015naa a2200265 a 4500
001 1062926
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1094/PDIS-06-21-1140-RE$2DOI
100 1 $aDE ARMAS, S.
245 $aPhylogeny and identification of Pantoea species associated with bulb rot and bacterial leaf blight of onion crops in Uruguay.$h[electronic resource]
260 $c2022
500 $aPublished Online:27 Mar 2022. Corresponding author: María I. Siri; Email: msiri@fq.edu.uy.
520 $aAbstract Onion is among the most consumed vegetables in Uruguay, grown in the northwestern and southern regions of the country. The onion supply presents interannual variations associated with significant postharvest losses, mainly caused by bacterial rots. Besides bulb rotting, onion leaf lesions as well as infections on seed-stalks during seed production may be devastating for some varieties under conducive conditions. This research aimed to identify the causal agents of bulb rots and leaf blight of onion crops in Uruguay. Symptomatic bulbs, seeds-stalks, and leaves were collected from commercial fields from 2015 to 2020. Bacterial colonies were isolated and identified at genera level using physiological tests and 16S rRNA gene sequence analysis. A collection of 59 Pantoea spp. isolates was obtained (11 from bulbs and 48 from leaves and seeds-stalks). Multilocus sequence analysis using four housekeeping genes (rpoB, gyrB, leuS, and fusA) allowed the assignment of the isolates to five Pantoea species: P. ananatis, P. agglomerans, P. allii, P. eucalypti, and P. vagans. The last two species were not previously reported as onion pathogens elsewhere. The ability to cause disease symptoms was tested by leaf inoculation and red onion scale assays. P. ananatis isolates showed the highest aggressiveness in both assays. Specific isolates from P. allii (MAI 6022), P. eucalypti (MAI 6036), P. vagans (MAI 6050), and Pantoea sp. (MAI 6049) ranked second in aggressiveness on onion leaves, whereas only three isolates belonging to P. eucalypti (MAI 6036 and MAI 6058) and P. agglomerans (MAI 6045) exhibited the same scale-clearing phenotype as P. ananatis. Leaf inoculation assays were also performed on a set of eight onion cultivars and breeding lines. Overall, P. ananatis MAI 6032 showed the highest aggressiveness in all tested cultivars, followed by P. eucalypti MAI 6036. The presence of new reported bacterial species leads to complex disease management and highlights the need for further studies on virulence factors and the epidemiology of these pathogens.
653 $aALLIUM CEPA
653 $aCENTER ROT
653 $aPANTOEA SPP
653 $aPATHOGEN DIVERSITY
700 1 $aGALVÁN, G. A.
700 1 $aLAPAZ, M. I.
700 1 $aGONZÁLEZ-BARRIOS, P.
700 1 $aVICENTE, E.
700 1 $aPIANZZOLA, M. J.
700 1 $aSIRI, M. I.
773 $tPlant Disease, 2022, volume 106, issue 4, pp. 1216-1225. doi: https://doi.org/10.1094/PDIS-06-21-1140-RE
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
|
|
Registro completo
|
|
Biblioteca (s) : |
INIA Treinta y Tres. |
|
Fecha actual : |
09/03/2018 |
|
Actualizado : |
24/09/2018 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
GARCÍA, J.A.; GARCÍA Y SANTOS, C.; ROSAS, J.E.; DUTRA, F.; GARDNER, D. |
|
Afiliación : |
JUAN AGUSTÍN GARCÍA, Centro Universitario Regional Este, Universidad de la República, Uruguay.; CARMEN GARCÍA Y SANTOS, Departamento de Toxicología, Facultad de Veterinaria, Universidad de la República, Uruguay.; JUAN EDUARDO ROSAS CAISSIOLS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO DUTRA, DILAVE (División Laboratorios Veterinarios), Regional Este, MGAP, Uruguay.; DALE GARDNER, USDA, ARX, Poisonuous Plant Research Laboratory, USA. |
|
Título : |
A survey of Senecio spp. affecting livestock in Uruguay and their associated pyrrolizidine alkaloid content. |
|
Fecha de publicación : |
2018 |
|
Fuente / Imprenta : |
Ciência Rural, feb., 2018, v.48(2), p. e20170621. |
|
ISSN : |
1678-4596 |
|
DOI : |
10.1590/0103-8478cr20170621 |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received 09.04.17; Approved 11.30.17 Returned by the author 12.27.17. CR-2017-0621.R1 |
|
Contenido : |
ABSTRACT:
In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved in the reported outbreaks of seneciosis. MenosABSTRACT:
In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved... Presentar Todo |
|
Palabras claves : |
ASTERACEAE; PLANT SECONDARY METABOLITES; PLANTAS TÓXICAS; POISONOUS PLANTS; SENECIOSIS. |
|
Thesagro : |
RUMIANTES; SENECIO. |
|
Asunto categoría : |
Q53 Contaminación y toxicología de alimentos para animales |
|
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8891/1/Rosas-Arb-2018-1.pdf
|
|
Marc : |
LEADER 02472naa a2200289 a 4500
001 1058223
005 2018-09-24
008 2018 bl uuuu u00u1 u #d
022 $a1678-4596
024 7 $a10.1590/0103-8478cr20170621$2DOI
100 1 $aGARCÍA, J.A.
245 $aA survey of Senecio spp. affecting livestock in Uruguay and their associated pyrrolizidine alkaloid content.$h[electronic resource]
260 $c2018
500 $aArticle history: Received 09.04.17; Approved 11.30.17 Returned by the author 12.27.17. CR-2017-0621.R1
520 $aABSTRACT: In Eastern Uruguay there has been a significant increase of seneciosis in grazing livestock with most affected localities related to counties neighboring the Brazilian border. A survey in 28 farms associated with poisoning outbreaks in grazing cattle in Eastern Uruguay was carried out. Fifty populations of Senecio plants were collected for alkaloid analysis and species identification. Four species were identified: S. oxyphyllus DC, S. madagascariensis Poir, S. brasiliensis (Spreng.) Less., and S. selloi DC. Alkaloids were identified by a combination of GC-MS and HPLC-MS analysis and included: retrorsine in S. oxyphyllus; retrorsine, usaramine, and senecivernine/senecionine in S. selloi; retrorsine, senecivernine/senecionine, integerrimine, and usaramine in S. madagascariensis; and integerrimine, retrorsine and senecionine in S. brasiliensis. Total mean alkaloid concentration was reported to be highest in S. brasiliensis (17.6mg/g) followed by S. oxyphyllus (6.2mg/g), S. selloi (1.8mg/g) and S. adagascariensis (0.6mg/g). Alkaloid concentrations were also reported to be higher in 2015 vs. 2016 probably due to a common environmental factor. The species S. oxyphyllus and S. madagascariensis were not previously recognized as toxic plants in Eastern Uruguay. Particularly, S. oxyphyllus was present in 82% of the farms surveyed and occurred in high density with relative high concentrations of pyrrolizidine alkaloids suggesting S. oxyphyllus may be the main species involved in the reported outbreaks of seneciosis.
650 $aRUMIANTES
650 $aSENECIO
653 $aASTERACEAE
653 $aPLANT SECONDARY METABOLITES
653 $aPLANTAS TÓXICAS
653 $aPOISONOUS PLANTS
653 $aSENECIOSIS
700 1 $aGARCÍA Y SANTOS, C.
700 1 $aROSAS, J.E.
700 1 $aDUTRA, F.
700 1 $aGARDNER, D.
773 $tCiência Rural, feb., 2018$gv.48(2), p. e20170621.
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA Treinta y Tres (TT) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
