|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
18/04/2023 |
Actualizado : |
18/04/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
RUBIO, L.; ARRUABARRENA, A.; SALVO, M.; CASTELLS, M.; BERTALMIO, A.; HERNÁNDEZ-RODRÍGUEZ, L.; BENÍTEZ-GALEANO, M.J.; MAESO, D.; COLINA, R.; RIVAS, F. |
Afiliación : |
LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA ARRUABARRENA PASCOVICH, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATÍAS SALVO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR Litoral Norte), Universidad de la República, Rivera 1350, Salto, CP 50000, Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LESTER HERNÁNDEZ RODRÍGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MARÍA J. BENÍTEZ-GALEANO, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR Litoral Norte), Universidad de la República, Rivera 1350, Salto, CP 50000, Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional Litoral Norte (CENUR Litoral Norte), Universidad de la República, Rivera 1350, Salto, CP 50000, Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Biological and molecular characterization of a resistance-breaking isolate of citrus tristeza virus from Uruguay and its effects on Poncirus trifoliata growth performance. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Archives of Virology, 2023, Volume 168, Issue 4, article 123. doi: https://doi.org/10.1007/s00705-023-05749-y |
ISSN : |
0304-8608 |
DOI : |
10.1007/s00705-023-05749-y |
Idioma : |
Inglés |
Notas : |
Article history: Received 24 November 2022; Accepted 11 February 2023; Published online 29 March 2023. -- Correspondence author: Rubio, L.; Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande. Camino al Terrible, Salto, Uruguay; email:lrubio@inia.org.uy -- Funding: This research was funded by the National Institute of Agricultural Research (INIA), Uruguay (project CT-22), through the National Program of Citrus Research. |
Contenido : |
Resistance-breaking (RB) isolates of citrus tristeza virus (CTV) can replicate and move systemically in Poncirus trifoliata, a rootstock widely used for management of decline caused by CTV and other purposes. In Uruguay, severe CTV isolates are prevalent, and an RB isolate (designated as RB-UY1) was identified. In order to predict the implications of this genotype circulating in citrus crops grafted on trifoliate rootstocks, the aim of this work was to determine the biological and molecular characteristics of this isolate, the efficiency of its transmission by Toxoptera citricida, and its effects on plant growth performance of P. trifoliata. Our results show that RB-UY1 can be classified as a mild isolate, that it is phylogenetically associated with the RB1 group, and that it is efficiently transmitted by T. citrida. They also suggest that the RB-UY1 isolate should not affect the performance of citrus crops grafted on trifoliate rootstocks, although some growth parameters of P. trifoliata seedlings were affected four years after inoculation. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. |
Palabras claves : |
Aphid transmission; Biological characterization; Growth performance; Poncirus trifoliata; RB isolate. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
Marc : |
LEADER 02703naa a2200325 a 4500 001 1064041 005 2023-04-18 008 2023 bl uuuu u00u1 u #d 022 $a0304-8608 024 7 $a10.1007/s00705-023-05749-y$2DOI 100 1 $aRUBIO, L. 245 $aBiological and molecular characterization of a resistance-breaking isolate of citrus tristeza virus from Uruguay and its effects on Poncirus trifoliata growth performance.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 24 November 2022; Accepted 11 February 2023; Published online 29 March 2023. -- Correspondence author: Rubio, L.; Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande. Camino al Terrible, Salto, Uruguay; email:lrubio@inia.org.uy -- Funding: This research was funded by the National Institute of Agricultural Research (INIA), Uruguay (project CT-22), through the National Program of Citrus Research. 520 $aResistance-breaking (RB) isolates of citrus tristeza virus (CTV) can replicate and move systemically in Poncirus trifoliata, a rootstock widely used for management of decline caused by CTV and other purposes. In Uruguay, severe CTV isolates are prevalent, and an RB isolate (designated as RB-UY1) was identified. In order to predict the implications of this genotype circulating in citrus crops grafted on trifoliate rootstocks, the aim of this work was to determine the biological and molecular characteristics of this isolate, the efficiency of its transmission by Toxoptera citricida, and its effects on plant growth performance of P. trifoliata. Our results show that RB-UY1 can be classified as a mild isolate, that it is phylogenetically associated with the RB1 group, and that it is efficiently transmitted by T. citrida. They also suggest that the RB-UY1 isolate should not affect the performance of citrus crops grafted on trifoliate rootstocks, although some growth parameters of P. trifoliata seedlings were affected four years after inoculation. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature. 653 $aAphid transmission 653 $aBiological characterization 653 $aGrowth performance 653 $aPoncirus trifoliata 653 $aRB isolate 700 1 $aARRUABARRENA, A. 700 1 $aSALVO, M. 700 1 $aCASTELLS, M. 700 1 $aBERTALMIO, A. 700 1 $aHERNÁNDEZ-RODRÍGUEZ, L. 700 1 $aBENÍTEZ-GALEANO, M.J. 700 1 $aMAESO, D. 700 1 $aCOLINA, R. 700 1 $aRIVAS, F. 773 $tArchives of Virology, 2023, Volume 168, Issue 4, article 123. doi: https://doi.org/10.1007/s00705-023-05749-y
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
08/06/2021 |
Actualizado : |
02/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
ESTEVEZ M.B.; CASAUX, M.L.; FRAGA, M.; FACCIO, R.; ALBORÉS, S. |
Afiliación : |
MARÍA BELÉN ESTEVEZ, Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay./Posgrado en Química, Facultad de Química, Universidad de la República, Montevideo, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO FACCIO, Departamento de Experimentación y Teoría de la Estructura de la Materia y sus Aplicaciones (DETEMA), Facultad de Química, Centro NanoMat and Grupo Física, Universidad de la República, Montevideo, Uruguay.; SILVANA ALBORÉS, Area de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo, Uruguay. |
Título : |
Biogenic silver nanoparticles as a strategy in the fight against multi-resistant salmonella enterica isolated from dairy calves. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Frontiers in Bioengineering and Biotechnology, April 2021, Volume 926, Article number 644014. OPEN ACCESS. Doi: https://doi.org/10.3389/fbioe.2021.644014 |
DOI : |
10.3389/fbioe.2021.644014 |
Idioma : |
Inglés |
Notas : |
Article history:Received: 19 December 2020/Accepted: 31 March 2021/Published: 26 April 2021,
This work was supported by the Comisión Sectorial de Investigación Científica (CSIC), Espacio Interdisciplinario (EI) y Comisión Académica de Posgrado (CAP) (Universidad de la República, Uruguay), Program for the Development of Basic Sciences (PEDECIBA-Química), and Agencia Nacional de Investigación e Innovación (ANII) Uruguay. |
Contenido : |
Abstract: Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise,the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures. MenosAbstract: Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise,the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which sugges... Presentar Todo |
Palabras claves : |
Antimicrobial; Biogenic nanoparticles; Confocal Raman Microscopy; Multi-resistance; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Salmonella Typhimurium; TERNEROS LECHEROS. |
Asunto categoría : |
E16 Enfermedades de los animales |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16669/1/fbioe-09-644014.pdf
https://www.frontiersin.org/articles/10.3389/fbioe.2021.644014/pdf
|
Marc : |
LEADER 02991naa a2200277 a 4500 001 1062117 005 2022-09-02 008 2021 bl uuuu u00u1 u #d 024 7 $a10.3389/fbioe.2021.644014$2DOI 100 1 $aESTEVEZ M.B. 245 $aBiogenic silver nanoparticles as a strategy in the fight against multi-resistant salmonella enterica isolated from dairy calves.$h[electronic resource] 260 $c2021 500 $aArticle history:Received: 19 December 2020/Accepted: 31 March 2021/Published: 26 April 2021, This work was supported by the Comisión Sectorial de Investigación Científica (CSIC), Espacio Interdisciplinario (EI) y Comisión Académica de Posgrado (CAP) (Universidad de la República, Uruguay), Program for the Development of Basic Sciences (PEDECIBA-Química), and Agencia Nacional de Investigación e Innovación (ANII) Uruguay. 520 $aAbstract: Infectious diseases are one of the most important health problems worldwide, one of the main causes being the development of multi-resistant microorganisms. Likewise,the zoonotic potential of some pathogens and their ability to transfer resistance mechanisms, reduce the therapeutic options in both humans and animals. Salmonella enterica is an important pathogen that affects a wide range of animal species and humans, being Salmonella Typhimurium one of the most frequent serotypes affecting cattle, causing enteritis, diarrhea, and septicemia. The search for alternative therapeutic approaches has gained importance since the emergence of multidrug resistance to antibiotics and periodic outbreaks of salmonellosis. In this sense, the discovery of new drugs and the development of new strategies, such as the use of nanoparticles with antimicrobial activity, are very promising. The aim of this work was the extracellular production of biogenic silver nanoparticles using fungal extracts and the evaluation of their antimicrobial activity against resistant and multi-resistant Salmonella Typhimurium strains. We here demonstrated the potential of the biogenic nanoparticles as effective bacteriostatic and bactericidal agents for use in biomedical applications. In addition, Confocal Raman Microscopy and Atomic Force Microscopy were used to advance the understanding of the antimicrobial mechanism of biogenic nanoparticles against these pathogenic strains, the results of which suggested that the nanoparticles produced damage in several bacterial cell structures. 653 $aAntimicrobial 653 $aBiogenic nanoparticles 653 $aConfocal Raman Microscopy 653 $aMulti-resistance 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL 653 $aSalmonella Typhimurium 653 $aTERNEROS LECHEROS 700 1 $aCASAUX, M.L. 700 1 $aFRAGA, M. 700 1 $aFACCIO, R. 700 1 $aALBORÉS, S. 773 $tFrontiers in Bioengineering and Biotechnology, April 2021, Volume 926, Article number 644014. OPEN ACCESS. Doi: https://doi.org/10.3389/fbioe.2021.644014
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|