02703naa a2200325 a 450000100080000000500110000800800410001902200140006002400360007410000140011024501990012426000090032350004760033252011680080865300230197665300320199965300230203165300240205465300150207870000210209370000140211470000170212870000180214570000300216370000270219370000140222070000150223470000140224977301140226310640412023-04-18       2023    bl uuuu     u00u1 u    #d  a0304-86087 a10.1007/s00705-023-05749-y2DOI1 aRUBIO, L.  aBiological and molecular characterization of a resistance-breaking isolate of citrus tristeza virus from Uruguay and its effects on Poncirus trifoliata growth performance.h[electronic resource]  c2023  aArticle history: Received 24 November 2022; Accepted 11 February 2023; Published online 29 March 2023. -- Correspondence author: Rubio, L.; Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental INIA Salto Grande. Camino al Terrible, Salto, Uruguay; email:lrubio@inia.org.uy  -- Funding: This research was funded by the National Institute of Agricultural Research (INIA), Uruguay (project CT-22), through the National Program of Citrus Research.  aResistance-breaking (RB) isolates of citrus tristeza virus (CTV) can replicate and move systemically in Poncirus trifoliata, a rootstock widely used for management of decline caused by CTV and other purposes. In Uruguay, severe CTV isolates are prevalent, and an RB isolate (designated as RB-UY1) was identified. In order to predict the implications of this genotype circulating in citrus crops grafted on trifoliate rootstocks, the aim of this work was to determine the biological and molecular characteristics of this isolate, the efficiency of its transmission by Toxoptera citricida, and its effects on plant growth performance of P. trifoliata. Our results show that RB-UY1 can be classified as a mild isolate, that it is phylogenetically associated with the RB1 group, and that it is efficiently transmitted by T. citrida. They also suggest that the RB-UY1 isolate should not affect the performance of citrus crops grafted on trifoliate rootstocks, although some growth parameters of P. trifoliata seedlings were affected four years after inoculation. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.  aAphid transmission  aBiological characterization  aGrowth performance  aPoncirus trifoliata  aRB isolate1 aARRUABARRENA, A.1 aSALVO, M.1 aCASTELLS, M.1 aBERTALMIO, A.1 aHERNÁNDEZ-RODRÍGUEZ, L.1 aBENÍTEZ-GALEANO, M.J.1 aMAESO, D.1 aCOLINA, R.1 aRIVAS, F.  tArchives of Virology, 2023, Volume 168, Issue 4, article 123. doi: https://doi.org/10.1007/s00705-023-05749-y