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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
02/04/2020 |
Actualizado : |
02/04/2020 |
Tipo de producción científica : |
Documentos |
Autor : |
FERREIRA, I.; NÚÑEZ, E.; QUEZADA, M.; CABRERA, D.; VIGNALE, B.; PRITSCH, C. |
Afiliación : |
ISABEL FERREIRA, Depto. Biología Vegetal, Facultad de Agronomía UdelaR.; EVELYN NÚÑEZ, Depto. Biología Vegetal, Facultad de Agronomía UdelaR.; MARIANELLA QUEZADA, Depto. Biología Vegetal, Facultad de Agronomía UdelaR.; CARLOS DANILO CABRERA BOLOGNA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BEATRIZ VIGNALE, Depto. Producción Vegetal EEFAS. Facultad de Agronomía UdelaR.; CLARA PRITSCH, Depto. Biología Vegetal, Facultad de Agronomía UdelaR. |
Título : |
Una propuesta de marcadores moleculares para la identificación varietal en Guayabo del País. |
Fecha de publicación : |
2019 |
Fuente / Imprenta : |
In: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Encuentro Nacional sobre Frutos Nativos, 9°. Fiesta Nacional del Guayabo del País, 1. Centro de Recreación de Paso Severino, Florida; 28-29 de marzo de 2019. Canelones (Uruguay): INIA. |
Páginas : |
p. 7. |
Serie : |
(Serie Actividades de Difusión; 789). |
ISSN : |
1688-9258 |
Idioma : |
Español |
Contenido : |
Los marcadores moleculares son herramientas que complementan la caracterización morfológica necesaria para la identificación varietal. En particular, son valiosos para resolver errores en la identificación de variedades, estimar las relaciones de parentesco y verificar la fidelidad clonal de las multiplicaciones.
Este trabajo apunta a desarrollar e identificar marcadores moleculares SSR y SRAP que permitan la identificación varietal en A. sellowiana. |
Palabras claves : |
Acca sellowiana; Cultivar; SRAP; SSR. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14355/1/SAD-789-Ferreira-I.-et-al..pdf
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Marc : |
LEADER 01431naa a2200265 a 4500 001 1060981 005 2020-04-02 008 2019 bl uuuu u00u1 u #d 022 $a1688-9258 100 1 $aFERREIRA, I. 245 $aUna propuesta de marcadores moleculares para la identificación varietal en Guayabo del País.$h[electronic resource] 260 $c2019 300 $ap. 7. 490 $a(Serie Actividades de Difusión; 789). 520 $aLos marcadores moleculares son herramientas que complementan la caracterización morfológica necesaria para la identificación varietal. En particular, son valiosos para resolver errores en la identificación de variedades, estimar las relaciones de parentesco y verificar la fidelidad clonal de las multiplicaciones. Este trabajo apunta a desarrollar e identificar marcadores moleculares SSR y SRAP que permitan la identificación varietal en A. sellowiana. 653 $aAcca sellowiana 653 $aCultivar 653 $aSRAP 653 $aSSR 700 1 $aNÚÑEZ, E. 700 1 $aQUEZADA, M. 700 1 $aCABRERA, D. 700 1 $aVIGNALE, B. 700 1 $aPRITSCH, C. 773 $tIn: INIA (Instituto Nacional de Investigación Agropecuaria); Programa Nacional Producción Frutícola. Encuentro Nacional sobre Frutos Nativos, 9°. Fiesta Nacional del Guayabo del País, 1. Centro de Recreación de Paso Severino, Florida; 28-29 de marzo de 2019. Canelones (Uruguay): INIA.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
Fecha actual : |
22/07/2018 |
Actualizado : |
28/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
WEINROTH, M.D.; SCOTT, H.M.; NORBY, B.; LONERAGAN, G.H.; NOYES, N.R.; ROVIRA, P.J.; DOSTER, E.; YANG, X.; WOERNER, D.R.; MORLEY, P.S.; BELK, K.E. |
Afiliación : |
M.D. WEINROTH, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); H.M. SCOTT, Universidad de Texas A&M; B. NORBY, Universidad de Michigan State (MSU); G.H. LONERAGAN, Texas Tech University; N.R. NOYES, Universidad de Colorado State (CSU); PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. DOSTER, Universidad de Colorado State (CSU); X. YANG, Universidad de California Davis (UCD); D.R. WOERNER, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); P. S. MORLEY, Universidad de Colorado State (CSU); K. E. BELK, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program). |
Título : |
Effects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Applied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18. |
Descripción física : |
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
ISSN : |
0099-2240 |
DOI : |
10.1128/AEM.00610-18 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. |
Contenido : |
ABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome.
© 2018 American Society for Microbiology. MenosABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, gen... Presentar Todo |
Palabras claves : |
ANTIBIOTIC RESISTANCE; ANTIMICROBIAL AGENTS; CATTLE; METAGENOMICS; POSTANTIBIOTIC EFFECT. |
Thesagro : |
FEEDLOT. |
Asunto categoría : |
-- L02 Alimentación animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12027/1/e00610-18.full.pdf
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Marc : |
LEADER 03064naa a2200361 a 4500 001 1058828 005 2018-11-28 008 2018 bl uuuu u00u1 u #d 022 $a0099-2240 024 7 $a10.1128/AEM.00610-18$2DOI 100 1 $aWEINROTH, M.D. 245 $aEffects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle.$h[electronic resource] 260 $c2018 300 $cThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. 500 $aArticle history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. 520 $aABSTRACT. Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome. © 2018 American Society for Microbiology. 650 $aFEEDLOT 653 $aANTIBIOTIC RESISTANCE 653 $aANTIMICROBIAL AGENTS 653 $aCATTLE 653 $aMETAGENOMICS 653 $aPOSTANTIBIOTIC EFFECT 700 1 $aSCOTT, H.M. 700 1 $aNORBY, B. 700 1 $aLONERAGAN, G.H. 700 1 $aNOYES, N.R. 700 1 $aROVIRA, P.J. 700 1 $aDOSTER, E. 700 1 $aYANG, X. 700 1 $aWOERNER, D.R. 700 1 $aMORLEY, P.S. 700 1 $aBELK, K.E. 773 $tApplied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18.
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