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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha : |
05/04/2022 |
Actualizado : |
01/09/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
SCHVARTZMAN, C.; FRESIA, P.; MURCHIO, S.; MUJICA, V.; DALLA RIZZA, M. |
Afiliación : |
CLAUDIA SCHVARTZMAN DISEGNI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; MARIA SARA MURCHIO VIGNOLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA VALENTINA MUJICA TELIZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
RNAi in Piezodorus guildinii (Hemiptera: Pentatomidae): transcriptome assembly for the development of pest control strategies. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
Frontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839 |
Páginas : |
13 p. |
DOI : |
10.3389/fpls.2022.804839 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022.
Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy
Al abrir el registro se visualiza el enlace al material suplementario. |
Contenido : |
Red-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. |
Palabras claves : |
DSRNA,; PEST CONTROL; RNA-SEQ; RNAI; STINK BUG; VATPASE A,. |
Asunto categoría : |
H10 Plagas de las plantas |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16649/1/Frontiers-Plant-Science-2022-Schvartzman.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/16650/1/Frontiers-Plant-Science-2022-Schvartzman-1.PDF
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Marc : |
LEADER 02536naa a2200277 a 4500 001 1062960 005 2022-09-01 008 2022 bl uuuu u00u1 u #d 024 7 $a10.3389/fpls.2022.804839$2DOI 100 1 $aSCHVARTZMAN, C. 245 $aRNAi in Piezodorus guildinii (Hemiptera$bPentatomidae): transcriptome assembly for the development of pest control strategies.$h[electronic resource] 260 $c2022 300 $a13 p. 500 $aArticle history: Received: 29 October 2021; Accepted: 28 February 2022; Published: 01 April 2022. Correspondence: Marco Dalla-Rizza, mdallarizza@inia.org.uy Al abrir el registro se visualiza el enlace al material suplementario. 520 $aRed-banded stink bug Piezodorus guildinii (P. guildinii) has been described as the most damaging stink bug regarding soybean crops, leading to seed injury, low germination percentages, and foliar retention, at low population densities. In recent years, RNA interference (RNAi), a conserved eukaryote silencing mechanism has been explored to develop species-selective pesticides. In this work, we evaluated RNAi in P. guildinii to develop new pest-control strategies. For this, we assembled and annotated a P. guildinii transcriptome from a pool of all developmental stages. Analysis of this transcriptome led to the identification of 56 genes related to the silencing process encompassing siRNA, miRNA, and piRNA pathways. To evaluate the functionality of RNAi machinery, P. guildinii adults were injected with 28 ng/mg of body weight of double stranded RNA (dsRNA) targeting vATPase A. A mortality of 35 and 51.6% was observed after 7 and 14 days, respectively, and a downregulation of vATPase A gene of 84% 72 h post-injection. In addition, Dicer-2 and Argonaute-2 genes, core RNAi proteins, were upregulated 1.8-fold 48 h after injection. These findings showed for the first time that RNAi is functional in P. guildinii and the silencing of essential genes has a significant effect in adult viability. Taken together, the work reported here shows that RNAi could be an interesting approach for the development of red-banded stink bug control strategies. 653 $aDSRNA, 653 $aPEST CONTROL 653 $aRNA-SEQ 653 $aRNAI 653 $aSTINK BUG 653 $aVATPASE A, 700 1 $aFRESIA, P. 700 1 $aMURCHIO, S. 700 1 $aMUJICA, V. 700 1 $aDALLA RIZZA, M. 773 $tFrontiers in Plant Science, 01 April 2022, volume13, article 804839. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2022.804839
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
03/05/2018 |
Actualizado : |
03/05/2018 |
Tipo de producción científica : |
Documentos |
Autor : |
CASTRO, M.; VÁZQUEZ, D.; MORALES, M.; CASTRO, B. |
Afiliación : |
MARINA CASTRO DERENYI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DANIEL VÁZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA XIMENA MORALES LORENZONI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BEATRIZ YANET CASTRO ALVARADO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
4. Resultados experimentales. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: Resultados experimentales de la evaluación nacional de cultivares de trigo calidad industrial: período 2017. La Estanzuela (UY): INIA; INASE, 2018. |
Páginas : |
p. 11-36. |
Idioma : |
Español |
Notas : |
Editado por el Equipo de Evaluación de Cultivares. Impreso por Unidad de Comunicación y Transferencia de Tecnología, INIA La Estanzuela. Convenio INASE-INIA. |
Palabras claves : |
DUREZAS; EXTRACCIÓN % DE CULTIVARES DE TRIGO; FALLING NUMBER; GLUTEN; ÍNDICE CALIDAD PANADERA (ICP); PESO DE MIL GRANOS; PESO HECTOLÍTRICO; PROTEÍNA; VALORES ALVEOGRÁFICOS; VALORES MIXOGRÁFICOS. |
Thesagro : |
EVALUACION DE CULTIVARES; TRIGO. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9437/1/Publicacion-Trigo-CALIDAD-2018-periodo-2017.p.11-36-Castro-et-al.pdf
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Marc : |
LEADER 01110naa a2200313 a 4500 001 1058514 005 2018-05-03 008 2018 bl uuuu u00u1 u #d 100 1 $aCASTRO, M. 245 $a4. Resultados experimentales.$h[electronic resource] 260 $c2018 300 $ap. 11-36. 500 $aEditado por el Equipo de Evaluación de Cultivares. Impreso por Unidad de Comunicación y Transferencia de Tecnología, INIA La Estanzuela. Convenio INASE-INIA. 650 $aEVALUACION DE CULTIVARES 650 $aTRIGO 653 $aDUREZAS 653 $aEXTRACCIÓN % DE CULTIVARES DE TRIGO 653 $aFALLING NUMBER 653 $aGLUTEN 653 $aÍNDICE CALIDAD PANADERA (ICP) 653 $aPESO DE MIL GRANOS 653 $aPESO HECTOLÍTRICO 653 $aPROTEÍNA 653 $aVALORES ALVEOGRÁFICOS 653 $aVALORES MIXOGRÁFICOS 700 1 $aVÁZQUEZ, D. 700 1 $aMORALES, M. 700 1 $aCASTRO, B. 773 $tIn: Resultados experimentales de la evaluación nacional de cultivares de trigo calidad industrial: período 2017. La Estanzuela (UY): INIA; INASE, 2018.
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