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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
06/12/2019 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
SILVEIRA, C.S.; MAYA,L.; CASAUX, M.L.; SCHILD, C.; CAFFARENA, D.; ARÁOZ, V.; COSTA, R.A. DA; MACÍAS-RIOSECO, M.; PERDOMO, Y,; CASTELLS, M.; COLINA, R.; FRAGA, M.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LETICIA MAYA, Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay.; MARÍA LAURA CASAUX, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS SCHILD, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA ARÁOZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RICARDO ALMEIDA DA COSTA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.; MATÍAS CASTELLS BAUER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay.; RODNEY COLINA, Laboratorio de Virología Molecular, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Diseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Brazilian Journal of Microbiology, 1 March 2020, Volume 51, Issue 1, Pages 357-368. OPEN ACCESS. Doi: https://doi.org/10.1007/s42770-019-00170-7 |
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DOI : |
10.1007/s42770-019-00170-7 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 26 February 2019 /Accepted: 3 October 2019. The work was partially funded by INIA?s grant PL015 N-15156. CSS, MLC, CS, DC, VA, RAC, MMR, and MC acknowledge support from ANII and INIA through MSc or PhD scholarships. |
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Contenido : |
Abstract:
Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector. MenosAbstract:
Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric c... Presentar Todo |
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Palabras claves : |
BOVINE VIRAL DIARRHEA VIRUS; INFECTIOUS DISEASE; LIVESTOCK PESTIVIRUS; PLATAFORMA SALUD ANIMAL; SOUTH AMERICA. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16716/1/s42770-019-00170-7.pdf
https://link.springer.com/content/pdf/10.1007/s42770-019-00170-7.pdf
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Marc : |
LEADER 03250naa a2200361 a 4500
001 1060512
005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s42770-019-00170-7$2DOI
100 1 $aSILVEIRA, C.S.
245 $aDiseases associated with bovine viral diarrhea virus subtypes 1a and 2b in beef and dairy cattle in Uruguay.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 26 February 2019 /Accepted: 3 October 2019. The work was partially funded by INIA?s grant PL015 N-15156. CSS, MLC, CS, DC, VA, RAC, MMR, and MC acknowledge support from ANII and INIA through MSc or PhD scholarships.
520 $aAbstract: Bovine viral diarrhea virus (BVDV, Pestivirus) causes significant economic losses to the livestock industry worldwide. Although serological surveys show that BVDV exposure is widespread in cattle in Uruguay, BVDV-associated diseases are greatly underreported. The aim of this work is to describe the epidemiological, clinical, pathological, and virological findings from spontaneous outbreaks of BVDV-associated diseases in cattle in Uruguay. Diagnostic investigations were performed during 6 spontaneous disease outbreaks on beef and dairy cattle farms in the departments of Colonia, Rio Negro, and Soriano between November 2016 and April 2018. Carcasses of 8 naturally deceased cattle from these outbreaks were necropsied and subjected to histological examination and immunohistochemistry to detect BVDV antigen in the tissues. Reverse transcription real-time PCR and genomic sequencing were also performed to identify BVDV at the species and subtype levels. Other ancillary diagnostic tests, including bacterial cultures, were performed on a case-by-case basis to rule in/out differential diagnoses based on initial clinicopathological presumptive diagnoses. BVDV-associated conditions that were diagnosed in the 8 cases included mucosal disease, transient postnatal BVDV infections associated with digestive/septicemic salmonellosis by Salmonella serovar typhimurium, Histophilus somni bronchopneumonia, urinary tract coinfections with Escherichia coli and Streptococcus sp., enteric coinfection with coccidia, and transplacental fetal infections and abortions with Neospora caninum coinfection. BVDV-1a and BVDV-2b were each identified in four of the eight cases. We conclude that BVDV-1a and BVDV-2b contribute significantly to disease and mortality in cattle in Uruguay. Future research should estimate the economic impact of BVDV in the Uruguayan livestock sector.
653 $aBOVINE VIRAL DIARRHEA VIRUS
653 $aINFECTIOUS DISEASE
653 $aLIVESTOCK PESTIVIRUS
653 $aPLATAFORMA SALUD ANIMAL
653 $aSOUTH AMERICA
700 1 $aMAYA,L.
700 1 $aCASAUX, M.L.
700 1 $aSCHILD, C.
700 1 $aCAFFARENA, D.
700 1 $aARÁOZ, V.
700 1 $aCOSTA, R.A. DA
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aPERDOMO, Y,
700 1 $aCASTELLS, M.
700 1 $aCOLINA, R.
700 1 $aFRAGA, M.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tBrazilian Journal of Microbiology, 1 March 2020, Volume 51, Issue 1, Pages 357-368. OPEN ACCESS. Doi: https://doi.org/10.1007/s42770-019-00170-7
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Registro original : |
INIA La Estanzuela (LE) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
30/09/2014 |
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Actualizado : |
11/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 2 |
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Autor : |
ANTÚNEZ , K.; MENDOZA, Y.; SANTOS, E.; INVERNIZZI, C. |
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Afiliación : |
YAMANDU MENDOZA SPINA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Differential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection. |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Journal of Apicultural Research, v. 52, n. 5, p. 277-234, 2013. |
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ISSN : |
0021-8839. |
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DOI : |
10.3896/IBRA.1.52.5.09 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013. |
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Contenido : |
Summary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of
honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae.
Resumen:
Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una
enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas
ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N.
ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas
fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del
traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y
menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron
artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina,
mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa
deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de
vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon
abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su
expresión diferencial podría estar asociada a la resistencia a N. ceranae. MenosSummary:
Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more
virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen.
The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this
difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of
Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the
number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were
selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of
immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin,
hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between
control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when
infected or control bees were compared between them. This protein possesses pleiotropic effects and is a cen... Presentar Todo |
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Palabras claves : |
NOSEMA CERANAE; NOSEMOSIS; PCR EN TIEMPO REAL; RESPUESTA INMUNE; VITELOGENINA. |
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Thesagro : |
APICULTURA; APIS MELLIFERA; ENFERMEDADES DE LAS ABEJAS. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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Marc : |
LEADER 04399naa a2200289 a 4500
001 1050723
005 2019-10-11
008 2013 bl uuuu u00u1 u #d
022 $a0021-8839.
024 7 $a10.3896/IBRA.1.52.5.09$2DOI
100 1 $aANTÚNEZ , K.
245 $aDifferential expression of vitellogenin in honey bees (Apis mellifera) with different degrees of Nosema ceranae infection.$h[electronic resource]
260 $c2013
500 $aArticle history: Received 3 October 2012 / Accepted subject to revision 12 January 2013 / Accepted for publication 18 February 2013.
520 $aSummary: Nosema apis and Nosema ceranae are causative agents of Nosemosis in the honey bee Apis mellifera, although N. ceranae may cause a more virulent disease. Selection of colonies with high tolerance to N. ceranae could be important for reducing problems caused by this pathogen. The aim of the present work was to evaluate the existence of honey bee colonies with different degrees of N. ceranae infection and test if this difference could be related to the immune response or vitellogenin expression. Healthy honey bee colonies were relocated to a plantation of Eucalyptus grandis to favour natural infection of N. ceranae. Fifteen and thirty days after relocation, the proportion of infected bees and the number of N. ceranae spores per field were quantified. The colonies with higher and lower levels of infection (HL and LL, respectively) were selected. Newly emerged bees from both colonies were artificially infected with N. ceranae and seven days after infection the expression of immune related genes and vitellogenin was evaluated by real time PCR. No significant differences were observed in expression of abaecin, hymenoptaecin, defensin, glucose dehydrogenase or lysozyme mRNA levels between infected bees from HL and LL colonies or between control bees from both colonies. Vitellogenin expression was higher in bees from the LL colony than in bees from the HL colony, when infected or control bees were compared between them. This protein possesses pleiotropic effects and is a central element in the life-history of honey bees. For that reason, its differential expression could be associated with resistance to N. ceranae. Resumen: Nosema apis y Nosema ceranae son agentes causales de Nosemosis en la abeja Apis mellifera aunque N. ceranae podría causar una enfermedad más virulenta. La selección de colonias resistentes a N. ceranae podría ser importante para la reducción de problemas ocasionados por este patógeno. El objetivo del presente trabajo fue evaluar la existencia de colonias con diferente grado de infección por N. ceranae y evaluar si esta diferencia podría estar relacionada a la respuesta inmune o a la expresión de vitelogenina. Colonias de abejas sanas fueron trasladadas a forestaciones de Eucalyptus grandis para favorecer la infección natural con N. ceranae. A los 15 y 30 días después del traslado, la proporción de abejas infectadas y el número de esporas de N. ceranae por campo fueron cuantificados. Las colonias con mayor y menor nivel de infección (HL y LL respectivamente) fueron seleccionadas. Abejas recientemente nacidas de ambas colonias se infectaron artificialmente con N. ceranae y siete días después de la infección se evaluó la expresión de genes relacionados con inmunidad y vitelogenina, mediante PCR en tiempo real. No se encontraron diferencias significativas en la expresión de abaecina, himenoptecina, defensina, glucosa deshidrogenasa o lisozima entre abejas infectadas de las colonias HLy LL o entre abejas control de ambas colonias. La expresión de vitelogenina resultó más baja en abejas pertenecientes a la colonia LL que en abejas pertenecientes a la colonia HL, cuando se compararon abejas infectadas o abejas control. Esta proteína posee efectos pleiotrópicos y es central en la vida de las abejas melíferas, por esta razón, su expresión diferencial podría estar asociada a la resistencia a N. ceranae.
650 $aAPICULTURA
650 $aAPIS MELLIFERA
650 $aENFERMEDADES DE LAS ABEJAS
653 $aNOSEMA CERANAE
653 $aNOSEMOSIS
653 $aPCR EN TIEMPO REAL
653 $aRESPUESTA INMUNE
653 $aVITELOGENINA
700 1 $aMENDOZA, Y.
700 1 $aSANTOS, E.
700 1 $aINVERNIZZI, C.
773 $tJournal of Apicultural Research$gv. 52, n. 5, p. 277-234, 2013.
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