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| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
25/01/2019 |
Actualizado : |
12/03/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
REDIERS, H.; BONNECARRERE, V.; RAINEY, P. B.; HAMONTS, K.; VANDERLEYDEN, J.; DE MOT, R. |
Afiliación : |
HANS REDIERS, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium; MARIA VICTORIA BONNECARRERE MARTINEZ, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium; INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PAUL B. RAINEY, Department of Plant Sciences,University of Oxford, United Kingdom; School of Biological Sciences, University of Auckland, New Zealand; KELLY HAMONTS, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium; JOS VANDERLEYDEN, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium; RENE DE MOT, Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Belgium. |
Título : |
Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15. |
Fecha de publicación : |
2003 |
Fuente / Imprenta : |
Applied and Environmental Microbiology, November 2003, volume 69, Issue 11, pages 6864-6874. |
ISSN : |
0099-2240 |
DOI : |
10.1128/AEM.69.11.6864-6874.2003 |
Idioma : |
Inglés |
Notas : |
Article history: Received 14 May 2003 //Accepted 5 August 2003. |
Contenido : |
Abstract.
Pseudomonas stutzeri A15 is a nitrogen-fixing bacterium isolated from paddy rice. Strain A15 is able to colonize and infect rice roots. This strain may provide rice plants with fixed nitrogen and hence promote plant growth. In this article, we describe the use of dapB-based in vivo expression technology to identify P. stutzeri A15 genes that are specifically induced during colonization and infection (cii). We focused on the identification of P. stutzeri A15 genes that are switched on during rice root colonization and are switched off during free-living growth on synthetic medium. Several transcriptional fusions induced in the rice rhizosphere were isolated. Some of the corresponding genes are involved in the stress response, chemotaxis, metabolism, and global regulation, while others encode putative proteins with unknown functions or without significant homology to known proteins.
Copyright © 2003, American Society for Microbiology. All Rights Reserved. |
Palabras claves : |
BACTERIA (MICROORGANISMS); BACTERIAL GENE; DAPB GENE; NITROGEN FIXATION. |
Thesagro : |
RICE. |
Asunto categoría : |
F01 Cultivo |
Marc : |
LEADER 01961naa a2200277 a 4500 001 1059455 005 2019-03-12 008 2003 bl uuuu u00u1 u #d 022 $a0099-2240 024 7 $a10.1128/AEM.69.11.6864-6874.2003$2DOI 100 1 $aREDIERS, H. 245 $aDevelopment and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15.$h[electronic resource] 260 $c2003 500 $aArticle history: Received 14 May 2003 //Accepted 5 August 2003. 520 $aAbstract. Pseudomonas stutzeri A15 is a nitrogen-fixing bacterium isolated from paddy rice. Strain A15 is able to colonize and infect rice roots. This strain may provide rice plants with fixed nitrogen and hence promote plant growth. In this article, we describe the use of dapB-based in vivo expression technology to identify P. stutzeri A15 genes that are specifically induced during colonization and infection (cii). We focused on the identification of P. stutzeri A15 genes that are switched on during rice root colonization and are switched off during free-living growth on synthetic medium. Several transcriptional fusions induced in the rice rhizosphere were isolated. Some of the corresponding genes are involved in the stress response, chemotaxis, metabolism, and global regulation, while others encode putative proteins with unknown functions or without significant homology to known proteins. Copyright © 2003, American Society for Microbiology. All Rights Reserved. 650 $aRICE 653 $aBACTERIA (MICROORGANISMS) 653 $aBACTERIAL GENE 653 $aDAPB GENE 653 $aNITROGEN FIXATION 700 1 $aBONNECARRERE, V. 700 1 $aRAINEY, P. B. 700 1 $aHAMONTS, K. 700 1 $aVANDERLEYDEN, J. 700 1 $aDE MOT, R. 773 $tApplied and Environmental Microbiology, November 2003, volume 69, Issue 11, pages 6864-6874.
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27. | | REDIERS, H.; BONNECARRERE, V.; RAINEY, P. B.; HAMONTS, K.; VANDERLEYDEN, J.; DE MOT, R. Development and application of a dapB-based in vivo expression technology system to study colonization of rice by the endophytic nitrogen-fixing bacterium Pseudomonas stutzeri A15. Applied and Environmental Microbiology, November 2003, volume 69, Issue 11, pages 6864-6874. Article history: Received 14 May 2003 //Accepted 5 August 2003.Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
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Associate Editor: Junping Chen.
The authors thank Edgardo Rey and Wanda Iriarte for technical assistance in field experiment and...Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
Biblioteca(s): INIA Las Brujas. |
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