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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
09/09/2014 |
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Actualizado : |
25/09/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
ROSAS, J.; BONNECARRERE, M.; PÉREZ DE VIDA, F. |
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Afiliación : |
JUAN EDUARDO ROSAS CAISSIOLS, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; MARIA VICTORIA BONNECARRERE MARTINEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
One-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.). |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2014, v. 17, no. 2, p.95-101 |
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ISSN : |
0717-3458 |
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DOI : |
10.1016/j.ejbt.2014.02.003 |
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Idioma : |
Inglés |
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Notas : |
Article history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014. |
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Contenido : |
Abstract
Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice. |
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Palabras claves : |
CLEARFIELD RICE; DNA - BASED RESISTANCE DIAGNOSIS; HERBICIDE RESISTANCE; KASP; RED RICE; SNP. |
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Thesagro : |
ARROZ ROJO; IMIDAZOLINONAS; RESISTENCIA A HERBICIDAS. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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Marc : |
LEADER 02123naa a2200289 a 4500
001 1050077
005 2018-09-25
008 2014 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $a10.1016/j.ejbt.2014.02.003$2DOI
100 1 $aROSAS, J.
245 $aOne-step, codominant detection of imidazolinone resistance mutations in weedy rice (Oryza sativa L.).$h[electronic resource]
260 $c2014
500 $aArticle history: received 25 June 2013; accepted 15 January 2014; available online 16 February 2014.
520 $aAbstract Weedy rice (Oryza sativa L.) is a noxious form of cultivated rice (O. sativa L.) associated with intensive rice production and dry seeding. A cost-efficient strategy to control this weed is the Clearfield rice production system, which combines imidazolinone herbicides with mutant imidazolinone-resistant rice varieties. However, imidazolinone resistance mutations can be introgressed in weedy rice populations by natural outcrossing, reducing the life span of the Clearfield technology. Timely and accurate detection of imidazolinone resistance mutations in weedy rice may contribute to avoiding the multiplication and dispersion of resistant weeds and to protect the Clearfield system. Thus, highly sensitive and specific methods with high throughput and low cost are needed. KBioscience?s Allele Specific PCR (KASP) is a codominant, competitive allele-specific PCR-based genotyping method. KASP enables both alleles to be detected in a single reaction in a closed-tube format. The aim of this work is to assess the suitability and validity of the KASP method for detection in weedy rice of the three imidazolinone resistance mutations reported to date in rice.
650 $aARROZ ROJO
650 $aIMIDAZOLINONAS
650 $aRESISTENCIA A HERBICIDAS
653 $aCLEARFIELD RICE
653 $aDNA - BASED RESISTANCE DIAGNOSIS
653 $aHERBICIDE RESISTANCE
653 $aKASP
653 $aRED RICE
653 $aSNP
700 1 $aBONNECARRERE, M.
700 1 $aPÉREZ DE VIDA, F.
773 $tElectronic Journal of Biotechnology, 2014$gv. 17, no. 2, p.95-101
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INIA Treinta y Tres (TT) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
06/06/2019 |
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Actualizado : |
06/06/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
SESSA, L.; ABREO, E.; BETTUCCI, L.; LUPO, S. |
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Afiliación : |
LUCÍA SESSA JUSID, INIA (Universidad de la República (UdelaR)/ Facultad de Ciencias; Instituto Nacional de Investigación Agropecuaria), Uruguay.; EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LINA BETTUCCI, Universidad de la República (UdelaR)/ Facultad de Ciencias; SANDRA LUPO, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
Diversity and virulence of Diaporthe species associated with wood disease symptoms in deciduous fruit trees in Uruguay. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Phytopathologia Mediterranea, 2017, vol. 56, Issue 3, pp. 431-444. |
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ISSN : |
0031-9465 // E- ISSN: 1593-2095 |
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DOI : |
10.14601/Phytopathol_Mediterr-20678 |
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Idioma : |
Inglés |
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Notas : |
This is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
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Contenido : |
SUMMARY.
Several Diaporthe species are recognized as causal agents of many plant disease symptoms, including twig and branch cankers, dieback, shoot blight, and root and fruit rots. In Uruguay, the proximity between apple, pear and peach orchards offers the possibility to study the presence of different Diaporthe spp. associated with
wood cankers across different deciduous fruit trees. Symptomatic twigs and branches of these orchard species were sampled, and isolates of Diaporthe were obtained. Selected isolates were used for cross inoculations in the three hosts. Seven Diaporthe spp. were identified, based on the internal transcribed spacer (ITS) region of rDNA and the translation elongation factor 1-alpha gene (EF1-α) phylogenies. The species were: Diaporthe amygdali, D. foeniculina, D. infecunda, D. eres, D. terebinthifolii, D. oxe and D. phaseolorum, while two isolates Diaporthe sp. 1 and Diaporthe sp. 4 could not be assigned to any species. Diaporthe infecunda, D. eres, D. terebinthifolii, D. phaseolorum and D. oxe on Pyrus communis and D. foeniculina on Malus domestica represent new records in these hosts in Uruguay, while D. oxe isolated from Prunus persica is a new record for this species. Diaporthe eres and D. phaseolorum were the most virulent species, posing the greatest risk due to their wide distribution and virulence in apple and peach trees. Although pear trees showed less symptomatic tissues and were less susceptible than peach and apple trees in the pathogenicity tests, they harboured seven of the species, and therefore should be considered as reservoirs of Diaporthe in Uruguayan orchards. Trees of the three hosts could be considered potential reciprocal sources of pathogenic Diaporthe spp.
© 2017 Author(s). MenosSUMMARY.
Several Diaporthe species are recognized as causal agents of many plant disease symptoms, including twig and branch cankers, dieback, shoot blight, and root and fruit rots. In Uruguay, the proximity between apple, pear and peach orchards offers the possibility to study the presence of different Diaporthe spp. associated with
wood cankers across different deciduous fruit trees. Symptomatic twigs and branches of these orchard species were sampled, and isolates of Diaporthe were obtained. Selected isolates were used for cross inoculations in the three hosts. Seven Diaporthe spp. were identified, based on the internal transcribed spacer (ITS) region of rDNA and the translation elongation factor 1-alpha gene (EF1-α) phylogenies. The species were: Diaporthe amygdali, D. foeniculina, D. infecunda, D. eres, D. terebinthifolii, D. oxe and D. phaseolorum, while two isolates Diaporthe sp. 1 and Diaporthe sp. 4 could not be assigned to any species. Diaporthe infecunda, D. eres, D. terebinthifolii, D. phaseolorum and D. oxe on Pyrus communis and D. foeniculina on Malus domestica represent new records in these hosts in Uruguay, while D. oxe isolated from Prunus persica is a new record for this species. Diaporthe eres and D. phaseolorum were the most virulent species, posing the greatest risk due to their wide distribution and virulence in apple and peach trees. Although pear trees showed less symptomatic tissues and were less susceptible than peach and apple trees in the pa... Presentar Todo |
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Palabras claves : |
DIAPORTHE ERES; DIAPORTHE OXE; MALUS DOMESTICA. |
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Thesagro : |
DIAPORTHE; PRUNUS PERSICA; PYRUS COMMUNIS. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12789/1/20678-47476-3-PB.pdf
http://www.fupress.net/index.php/pm/article/view/20678/20627
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Marc : |
LEADER 02819naa a2200265 a 4500
001 1059827
005 2019-06-06
008 2017 bl uuuu u00u1 u #d
022 $a0031-9465 // E- ISSN: 1593-2095
024 7 $a10.14601/Phytopathol_Mediterr-20678$2DOI
100 1 $aSESSA, L.
245 $aDiversity and virulence of Diaporthe species associated with wood disease symptoms in deciduous fruit trees in Uruguay.$h[electronic resource]
260 $c2017
500 $aThis is an open access article distributed under the terms of the Creative Commons Attribution License (CC-BY-4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
520 $aSUMMARY. Several Diaporthe species are recognized as causal agents of many plant disease symptoms, including twig and branch cankers, dieback, shoot blight, and root and fruit rots. In Uruguay, the proximity between apple, pear and peach orchards offers the possibility to study the presence of different Diaporthe spp. associated with wood cankers across different deciduous fruit trees. Symptomatic twigs and branches of these orchard species were sampled, and isolates of Diaporthe were obtained. Selected isolates were used for cross inoculations in the three hosts. Seven Diaporthe spp. were identified, based on the internal transcribed spacer (ITS) region of rDNA and the translation elongation factor 1-alpha gene (EF1-α) phylogenies. The species were: Diaporthe amygdali, D. foeniculina, D. infecunda, D. eres, D. terebinthifolii, D. oxe and D. phaseolorum, while two isolates Diaporthe sp. 1 and Diaporthe sp. 4 could not be assigned to any species. Diaporthe infecunda, D. eres, D. terebinthifolii, D. phaseolorum and D. oxe on Pyrus communis and D. foeniculina on Malus domestica represent new records in these hosts in Uruguay, while D. oxe isolated from Prunus persica is a new record for this species. Diaporthe eres and D. phaseolorum were the most virulent species, posing the greatest risk due to their wide distribution and virulence in apple and peach trees. Although pear trees showed less symptomatic tissues and were less susceptible than peach and apple trees in the pathogenicity tests, they harboured seven of the species, and therefore should be considered as reservoirs of Diaporthe in Uruguayan orchards. Trees of the three hosts could be considered potential reciprocal sources of pathogenic Diaporthe spp. © 2017 Author(s).
650 $aDIAPORTHE
650 $aPRUNUS PERSICA
650 $aPYRUS COMMUNIS
653 $aDIAPORTHE ERES
653 $aDIAPORTHE OXE
653 $aMALUS DOMESTICA
700 1 $aABREO, E.
700 1 $aBETTUCCI, L.
700 1 $aLUPO, S.
773 $tPhytopathologia Mediterranea, 2017, vol. 56, Issue 3, pp. 431-444.
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