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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
13/11/2015 |
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Actualizado : |
13/11/2015 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LADO, J.; CRONJE, P.; ALQUÉZAR, B.; PAGE, A.; MANZI, M.; GÓMEZ-CADENAS, A.; STEAD, A.D.; ZACARÍAS, L.; RODRIGO, M.J. |
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Afiliación : |
JOANNA LADO LINDNER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Fruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Physiologia Plantarum, 2015, v.154, no. 4, p. 469-484. |
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Serie : |
0031-9317 |
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DOI : |
10.1111/ppl.12332 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by
influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase.
Physiol. Plant. MenosABSTRACT.
The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast
development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated diff... Presentar Todo |
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Thesagro : |
CITRUS; CITRUS PARADISI. |
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Asunto categoría : |
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Marc : |
LEADER 03223naa a2200265 a 4500
001 1053867
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008 2015 bl uuuu u00u1 u #d
024 7 $a10.1111/ppl.12332$2DOI
100 1 $aLADO, J.
245 $aFruit shading enhances peel color, carotenes accumulation and chromoplast differentiation in red grapefruit.$h[electronic resource]
260 $c2015
490 $a0031-9317
520 $aABSTRACT. The distinctive color of red grapefruits is due to lycopene, an unusual carotene in citrus. It has been observed that red ?Star Ruby? (SR) grapefruits grown inside the tree canopy develop a more intense red coloration than those exposed to higher light intensities. To investigate the effect of light on SR peel pigmentation, fruit were bagged or exposed to normal photoperiodic conditions, and changes in carotenoids, expression of carotenoid biosynthetic genes and plastid ultrastructure in the peel were analyzed. Light avoidance accelerated chlorophyll breakdown and induced carotenoid accumulation, rendering fruits with an intense coloration. Remarkably, lycopene levels in the peel of shaded fruits were 49-fold higher than in light-exposed fruit while concentrations of downstream metabolites were notably reduced, suggesting a bottleneck at the lycopene cyclization in the biosynthetic pathway. Paradoxically, this increment in carotenoids in covered fruit was not mirrored by changes in mRNA levels of carotenogenic genes, which were mostly up-regulated by light. In addition, covered fruits experienced profound changes in chromoplast differentiation, and the relative expression of genes related to chromoplast development was enhanced. Ultrastructural analysis of plastids revealed an acceleration of chloroplasts to chromoplast transition in the peel of covered fruits concomitantly with development of lycopene crystals and plastoglobuli. In this sense, an accelerated differentiation of chromoplasts may provide biosynthetic capacity and a sink for carotenoids without involving major changes in transcript levels of carotenogenic genes. Light signals seem to regulate carotenoid accumulation at the molecular and structural level by influencing both biosynthetic capacity and sink strength. Abbreviations ? 𝛽CHX, 𝛽-carotene hydroxylase; 𝛽LCY, lycopene cyclase 𝛽; ABA, abscisic acid; C, covered; Chl, chlorophyll; DXS, 1-deoxy-D-xylulose-5-phosphate synthase; FIB, fibrillin; FW, fresh weight; GGPP, geranyl geranyl pyrophosphate; GGPPS, geranyl geranyl pyrophosphate synthase; HDR, hydroxymethylbutenyl diphosphate reductase; HPLC, high-performance liquid chromatography; MEP, methyl-D-erythritol-4-phosphate; NC, non-covered; PCR, polymerase chain reaction; PDS, phytoene desaturase; PSY, phytoene synthase; sHSP, small heat shock protein; SR, Star Ruby; ZDS, 𝜁-carotene desaturase. Physiol. Plant.
650 $aCITRUS
650 $aCITRUS PARADISI
700 1 $aCRONJE, P.
700 1 $aALQUÉZAR, B.
700 1 $aPAGE, A.
700 1 $aMANZI, M.
700 1 $aGÓMEZ-CADENAS, A.
700 1 $aSTEAD, A.D.
700 1 $aZACARÍAS, L.
700 1 $aRODRIGO, M.J.
773 $tPhysiologia Plantarum, 2015$gv.154, no. 4, p. 469-484.
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| 1. |  | GONZÁLEZ, G. Principios y estrategias del mejoramiento genético en ovinos ln: Foro Mejoramiento Genético Animal en el Uruguay: en vísperas del Mercosur, 17-18 agosto 1991, Montevideo, Uruguay. GIANOLA, D. (Ed.). Resúmenes. Montevideo (Uruguay): INIA, 1991. p. 30-33 (INIA Serie Técnica ; 12) Instituto Nacional de Investigación Agropecuaria (INIA)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 2. | | GONZÁLEZ, G.E. Cruzamientos en ganado de carne 2a. ed In: CARÁMBULA, M.; VAZ MARTINS, D.; INDARTE, E. (Eds.). Pasturas y producción animal en áreas de ganaderia extensiva. 2a. reimpresión . Montevideo (Uruguay): INIA, 1997. p. 179-194 (INIA Serie Técnica ; 13)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela; INIA Las Brujas; INIA Tacuarembó. |
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| 6. | | QUINTANS, G.; SALTA, V.; DAMBRAUSKAS, G.; GONZÁLEZ, G.E. Efecto del destete temporario sobre el comportamiento reproductivo en vacunos. ln: JORNADAS TÉCNICAS DE LA FACULTAD DE AGRONOMÍA, 1988, Montevideo, Uruguay. Memorias: Ciencias Biológicas, Ciencias Sociales, Producción Vegetal, Producción Animal, Suelos y Aguas. Montevideo (Uruguay): Facultad de Agronomía, 1988. p. 105-108.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA Treinta y Tres. |
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| 7. | | PREVE, F.; ABELLA, I.; PEREIRA, D.; HEINZEN, H.; GONZÁLEZ, G.; PÉREZ, A. Lanas naturales y con bajo contenido de pesticidas. Agrociencia, 2009, v. 13, no. 3, p. 86| Biblioteca(s): INIA Tacuarembó. |
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| 8. | | BALMELLI, G.; RESQUIN, F.; SIMETO, S.; TORRES, D.; NUÑEZ, P.; RODRIGUEZ, F.; GONZALEZ, G. Variabilidad genética en susceptibilidad a Teratosphaeria pseudoeucalypti en eucaliptos colorados. In: JORNADA TÉCNICA DE PROTECCIÓN FORESTAL, 6., 2013, TACUAREMBÓ, UY; BALMELLI, G.; SIMETO, S.; MARTINEZ, G.; GOMEZ, D. (Ed.). Montevideo, UY: INIA, 2014. 85-91 (INIA Serie Técnica; 213)| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA Tacuarembó. |
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| 9. | | GADEA, M.P.; VARELA, G.; BALSEIRO, V.; GONZÁLEZ, S.; MOTA, M.I.; GONZÁLEZ, G.; PARDO, L.; CATTÁNEO, M.; CORDEIRO, N.; SCHELOTTO, F.; GADEA, M.P. Detección y caracterización de Escherichia coli productor de toxina Shiga a partir de casos clínicos de alimentos y de animales en Uruguay. Carnes y Alimentos, 2009, v. 10, no. 32, p. 34-48.| Biblioteca(s): INIA Tacuarembó. |
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| 10. | | ALGORTA, I.; AZAMBUJA, T.; DÍAZ, A.; ERRO, L.; ESTABLE, L.; GONZÁLEZ, G.; HERWIG, G.; MARTÍNEZ, J.; MORIYAMA, S.; OLAIZOLA, V.; OSORIO, F.; PACHECO, P.; PÉREZ, C.; SANTÍN, V.; TRUJILLO, M.; VODANOVICH, V.; ZELMONOVICH, C. Grupo interinstitucional de promoción del consumo saludable de frutas y verduras. (Abs.1, Sección: Generales). IN: CONGRESO NACIONAL DE HORTI-FRUTICULTURA, 13. AÑO DE LA AGRICULTURA FAMILIAR DE LA FAO. "SOSTENIBILIDAD DE LA PRODUCCIÓN HORTIFRUTÍCOLA FAMILAR". 3-6 SETIEMBRE 2014, MONTEVIDEO (UY). Trabajos presentados. Montevideo (UY): INIA; SUHF, 2014. p. 17| Tipo: Abstracts/Resúmenes |
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