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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
26/01/2024 |
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Actualizado : |
26/01/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. |
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Afiliación : |
RONALDO JOSÉ PICCOLI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; LEONARDO GRUCHOUSKEI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDRÉ LUIS VRIESMAN BENINCA, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MARINA MAURENTE BERÓN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ARTHUR COLOMBARI CHENG, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JOICE APARECIDA DE ANDRADE, Animal Parasitology Laboratory, Federal University of Paraná, PR, Palotina, Brazil; MAYANE FACCIN, Veterinary Pathobiology Department, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, United States; ALINE PATRÍCIA GRZEGOZEVSKI, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; GEÓRGIA CAROLINA ROHDEN DA SILVA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; JAQUELINE COELHO FRANÇA, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; NELSON LUIS MELLO FERNANDES, Immunological Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ANDERSON LUIZ DE CARVALHO, Veterinary Hospital, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; ALINE DE MARCO VIOTT, Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil. |
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Título : |
Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. |
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Complemento del título : |
Original full article. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. |
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ISSN : |
1983-0246 |
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DOI : |
10.0.94.6/bjvp.1983-0246.v16i3p159-166 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br -- |
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Contenido : |
ABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved. MenosABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections ... Presentar Todo |
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Palabras claves : |
Diagnosis; Immunohistochemistry; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Psittacosis; Zoonosis. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://bjvp.org.br/wp-content/uploads/2023/10/BJVP_v16n3_159-166.pdf
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Marc : |
LEADER 03192naa a2200361 a 4500
001 1064440
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a1983-0246
024 7 $a10.0.94.6/bjvp.1983-0246.v16i3p159-166$2DOI
100 1 $aPICCOLI, R.J.
245 $aDetection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species.$h[electronic resource]
260 $c2023
500 $aArticle history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of Paraná, PR, Palotina, Brazil; email:alinedemarco@yahoo.com.br --
520 $aABSTRACT.- Chlamydiosis is a zoonotic disease that affects several animal species. Therefore, reliable detection techniques are essential for efficient control of the disease. Fluorescence in situ hybridization (FISH) for Chlamydia sp. was applied to 137 formalin-fixed, paraffin-embedded liver sections from native and exotic birds. The samples were divided into two groups: retrospective (n=57) and prospective (n=80). The probe was designed based on an annealing sequence that targets the Major Outer Membrane Protein coding gene. Livers previously confirmed for Chlamydia psittaci by polymerase chain reaction (PCR) and immunohistochemistry (IHC) were used as positive controls. Also, 47 randomly selected samples from the prospective group were submitted to PCR for Chlamydia psittaci for confirmation. From all 137 samples, 67% (92/137) were positive for Chlamydia sp. through FISH, from which 39% (36/57) and 61% (56/80) were from the retrospective and prospective groups, respectively. From the samples of the prospective group submitted for PCR confirmation, 83% (39/47) of them had a positive correlation with FISH results. When considering the number of targeted microorganisms per 400x field, 42,39% (39/92) of the samples had up to five microorganisms, 14,13% (13/92) had from six to ten, and 43,47% (40/92) of the samples had 11 or more microorganisms per field. FISH is a specific and reliable method to identify Chlamydia sp. in histologic sections of the liver, providing an additional tool for detecting avian chlamydiosis. © 2023, Brazilian Association of Veterinary Pathology. All rights reserved.
653 $aDiagnosis
653 $aImmunohistochemistry
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aPsittacosis
653 $aZoonosis
700 1 $aGRUCHOUSKEI, L.
700 1 $aBENINCA, A.L.V.
700 1 $aMAURENTE BERÓN, M.
700 1 $aCHENG, A.C.
700 1 $aDE ANDRADE, J.A.
700 1 $aFACCIN, M.
700 1 $aGRZEGOZEVSKI, A.P.
700 1 $aDA SILVA, G.C.R.
700 1 $aFRANÇA, J.C.
700 1 $aFERNANDES, N.L.M.
700 1 $aDE CARVALHO, A.L.
700 1 $aVIOTT, A.M.
773 $tBrazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS.
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| Registros recuperados : 278 | |
| 62. |  | SCHILD, C.; CAFFARENA, D.; RIET-CORREA, F.; GIANNITTI, F. Enfermedad articular degenerativa de las rodillas en una vaca. In: JORNADAS URUGUAYAS DE BUIATRÍA, 43., 2015, PAYSANDÚ, UY.; GIANNEECHINI, E.; MATTO, C. (Ed.). [Trabajos presentados]. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría. p.221-223.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 66. | | CASTELLS, M.; CAFFARENA, D.; CASAUX, L.; SCHILD, C.; GIANNITTI, F.; MIÑO, S.; RIET-CORREA, F.; PARREÑO, V.; COLINA, R. Determinación de la prevalencia y variabilidad genética de rotavirus: coronavirus, torovirus, norovirus y astrovirus. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 230-233.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 67. | | PICCOLI, R.J.; GRUCHOUSKEI, L.; BENINCA, A.L.V.; MAURENTE BERÓN, M.; CHENG, A.C.; DE ANDRADE, J.A.; FACCIN, M.; GRZEGOZEVSKI, A.P.; DA SILVA, G.C.R.; FRANÇA, J.C.; FERNANDES, N.L.M.; DE CARVALHO, A.L.; VIOTT, A.M. Detection of Chlamydia sp. by fluorescence in situ hybridization (FISH) in histologic sections of the liver from exotic and native avian species. Original full article. Brazilian Journal of Veterinary Pathology, 2023, Volume 16, Issue 3, Pages 159-166. https://10.0.94.6/bjvp.1983-0246.v16i3p159-166 -- OPEN ACCESS. Article history: Submitted: 27 July 2023, Accepted: 15 September 2023. -- Document type: Article Bronze Open Access. -- Correspondence: Viott, A.M.; Animal Pathology Laboratory, Veterinary Sciences Department, Federal University of...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 68. | | COSTA, R.A. DA; SCHILD, C.; CAFFARENA, D.; GIANNITTI, F.; BANCHERO, G.; DEL PINO, M.L.; RIET-CORREA, F. High frequency of cutaneous squamous cell carcinoma in Friesian Milchschaf sheep in Uruguay. [Alta frequência de carcinoma cutâneo de células escamosas em ovinos da raça Frisona Milchschaf no Uruguai]. Pesquisa Veterinaria Brasileira, 1 January 2019, Volume 39, Issue 4, Pages 251-254. [Open Access]. Article history: Received on December 3, 2018.//Accepted for publication on December 12, 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 69. | | MACHADO, M.; MARTÍNEZ, R.; ANDRES, S.; SUMARAH, M.W.; RENAUD, J.B.; ARMIÉN, A.G.; BARROS, C.S.L.; RIET-CORREA, F.; MENCHACA, A.; SCHILD, C. Poisoning by Baccharis coridifolia in early-weaned beef calves: pathological study and new macrocyclic trichothecene identification. Toxins. 2023; 15(12):681. https://doi.org/10.3390/toxins15120681 -- OPEN ACCESS. Article history: Submission received: 19 October 2023; Revised: 20 November 2023; Accepted: 27 November 2023; Published: 1 December 2023. -- Supplementary Materials: The following supporting information can be downloaded at:...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 70. | | DORSCH, M.; CASAUX, M.L.; CALLEROS, L.; ARÁOZ, V.; CAFFARENA, D.; MONESIGLIO, M.C.; BARCELLOS, M.; SILVEIRA, C.S.; PERDOMO, T.; BANCHERO, G.; UZAL, F.A.; FRAGA, M.; GIANNITTI, F. Corrigendum to "Placentitis and abortion caused by a multidrug resistant strain of Campylobacter fetus subspecies fetus in a sheep in Uruguay". (Revista Argentina de Microbiologia (2022) 54(1) (25-30), (S0325754121000389), (10.1016/j.ram.2021.02.005)). Erratum. Revista Argentina de Microbiologia. 2023, Volume 55, Issue 4, Pages 397-398. https://doi.org/10.1016/j.ram.2023.11.002 -- OPEN ACCESS. Article history: Available online 21 December 2023; Version of Record 21 December 2023. -- Correspondence: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 72. | | RABAZA, A.; BANCHERO, G.; CAJARVILLE,C.; ZUNINO, P.; BRITO, A.; REPETTO, J.; FRAGA, M. Effects of feed withdrawal duration on animal behaviour, rumen microbiota and blood chemistry in feedlot cattle: implications for rumen acidosis. Animal, Volume 14 , Issue 1 , January 2020 , pp. 66 - 77. DOI: https://doi.org/10.1017/S1751731119001538 Article history: Received 22 October 2018 // Accepted 13 June 2019.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA La Estanzuela. |
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| 73. | | CAFFARENA, D.; SCHILD, C.; RIET-CORREA, F.; GIANNITTI, F. Estenosis yeyunal congenita en una ternera holstein con sindrome del ternero débil. In: JORNADAS URUGUAYAS DE BUIATRÍA, 43., 2015, PAYSANDÚ, UY.; GIANNEECHINI, E.; MATTO, C. (Ed.). [Trabajos presentados]. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría. p. 224-226.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 74. | | MAYA, L.; PUENTES. R.; RIET-CORREA, F.; GIANNITTI, F.; RIVERO, R.; GINNNEECHINI, E.; FURTADO FLORES, E.; COLINA, R. Caracterización genética del virus de la diarrea viral bovina en Uruguay. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p. 183-185.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 75. | | COSTA, R.A. DA; CORBELLINI, L.G.; CASTRO, E.; RIET-CORREA, F. Impacto de la fasciola hepática en le Industria Cárnica bovina en Uruguay. In: Jornadas Uruguayas de Buiatría, 46., 2018, Paysandú, Uy., Matto, C.; Delpiazzo, R.(Ed.). Trabajos presentados. Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2018. p.199-201.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 78. | | RIET-CORREA, F.; MACHADO, M.; MICHELOUD, J. F. Plants causing poisoning outbreaks of livestock in South America: A review. Toxicon: X, 2023, Volume 17, article 100150. OPEN ACCESS. doi: https://doi.org/10.1016/j.toxcx.2023.100150 Article history: Received 12 December 2022; Revised 12 January 2023; Accepted 17 January 2023; Available online 21 January 2023; Version of Record 24 January 2023; To be Pubished March 2023. -- Corresponding author: Riet-Correa, F.;...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 79. | | MACHADO, M.; OLIVEIRA, L.G.S.; SCHILD, C.; BOABAID, F.; LUCAS, M.; BURONI, F.; CASTRO, M. B.; RIET-CORREA, F. Lantana camara poisoning in cattle that took refuge during a storm in a forest invaded by this plant. Toxicon, 2023, Volume 229, article 107124. https://doi.org/10.1016/j.toxicon.2023.107124 Article history: Received 8 March 2023; Received in revised form 7 April 2023; Accepted 10 April 2023; Available online 11 April 2023. -- Corresponding author: Riet-Correa, F.; Instituto Nacional de Investigación Agropecuaria,...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 278 | |
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