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41. | | MACHADO, M.; MARTÍNEZ, R.; ANDRES, S.; SUMARAH, M.W.; RENAUD, J.B.; ARMIÉN, A.G.; BARROS, C.S.L.; RIET-CORREA, F.; MENCHACA, A.; SCHILD, C. Poisoning by Baccharis coridifolia in early-weaned beef calves: pathological study and new macrocyclic trichothecene identification. Toxins. 2023; 15(12):681. https://doi.org/10.3390/toxins15120681 -- OPEN ACCESS. Article history: Submission received: 19 October 2023; Revised: 20 November 2023; Accepted: 27 November 2023; Published: 1 December 2023. -- Supplementary Materials: The following supporting information can be downloaded at:...Biblioteca(s): INIA Las Brujas. |
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47. | | RABAZA, A.; FRAGA, M.; MENDOZA, A.; GIANNITTI, F. A meta-analysis of the effects of colostrum heat-treatment on its viscosity and IgG concentration, and the transfer of passive immunity in newborn dairy calves. Journal of Dairy Science, 2023. https://doi.org/10.3168/jds.2022-22555 -- [Article In Press]. OPEN ACCESS. Article history: Received July 20, 2022; Accepted April 19, 2023; Published online May 08, 2023. -- Corresponding authors: Ana Rabaza, arabaza@pasteur.edu.uy; Federico Giannitti, fgiannitti@inia.org.uy -- License: This is an open access...Biblioteca(s): INIA Las Brujas. |
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48. | | LOPES, J.F.; CANOZZI, M.E.A.; GONÇALVES, T.L.; DA ROCHA, M.K.; SARTORI, E.D.; SESSIM, A.G.; DE PAULA PEREIRA, I.; DE AGUIAR, L.K.; PAPARAS, D.; MENEGASSI, S.R.O.; KOETZ, C.; OAIGEN, R.P.; ZAGO, D.; BARCELLOS, J.O.J. Price determinants of beef bulls sold in livestock auctions. Animal production systems and agribusiness. Revista Brasileira de Zootecnia, 2023, Volume 52, e20210227. https://doi.org/10.37496/rbz5220210227 -- OPEN ACCESS. Article history: Received 17 December 2021, Accepted 2 February 2023, Publication in this collection 09 October 2023, Date of issue 2023. -- Document type: Article Gold Open Access. -- Correspondence: Barcellos, J.O.J.; Universidade...Biblioteca(s): INIA Las Brujas. |
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49. | | BÓ, G.A.; MENCHACA, A. Prohibition of hormones in animal reproduction: what to expect and what to do?. Thematic Section: 36th Annual Meeting of the Brazilian Embryo Technology Society (SBTE). Animal Reproduction, 2023, Volume 20, Issue 2, e20230067. https://doi.org/10.1590/1984-3143-AR2023-0067 -- OPEN ACCESS. Article history: Received May 15, 2023; Accepted: July 18, 2023. -- Financial support: GAB received funding for this research from Fondo Nacional de Ciencia y Tecnología (FONCYT PICT 2017-4550), Instituto de Investigación...Biblioteca(s): INIA Las Brujas. |
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52. | | CASAUX, M.L.; D'ALESSANDRO, B.; VIGNOLI, R.; FRAGA, M. Phenotypic and genotypic survey of antibiotic resistance in Salmonella enterica isolates from dairy farms in Uruguay. Frontiers in Veterinary Science, 2023, Volume 10, article 1055432. OPEN ACCESS. doi: https://doi.org/10.3389/fvets.2023.1055432 Article history: Received 27 September 2022; Accepted 15 February 2023; Published 09 March 2023. -- Correspondence author: María Laura Casaux, lcasaux@inia.org.uy; Martín Fraga, mfraga@inia.org.uy -- Edited by: Julio Alvarez, VISAVET...Biblioteca(s): INIA Las Brujas. |
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53. | | SAWCHIK, J.; FERNANDEZ, E.; FERRARO, B.; GUTIERREZ, N.; OVIEDO, S. Plan Estratégico 2021-2025. Visión 2030. Montevideo (UY): INIA, 2023. 155 p. (Temas Institucionales; 17) Miembros de la Junta Directiva de INIA: Ing. Agr. José Eduardo Bonica (presidente), Ing. Agr. Walter Baethgen (vicepresidente), Ing. Agr. Martín Gortari Fernández, Ing. Agr. Rafael Normey, Ing. Agr. Alejandro Henry, Ing. Agr. Diego...Biblioteca(s): INIA Las Brujas. |
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55. | | FERNÁNDEZ, M.; CASAUX, M.L.; FRAGA, M.; VIGNOLI, R.; BADO, I.; ZUNINO, P.; UMPIÉRREZ, A. Shiga toxin-producing Escherichia coli (STEC) associated with calf mortality in Uruguay. Microorganisms. 2023, Volume 11, Issue 7, p. 1704. https://doi.org/10.3390/microorganisms11071704 ---- OPEN ACCESS. Article history: Received 17 May 2023; Revised 5 June 2023; Accepted 8 June 2023; Published 29 June 2023. -- Academic Editors: Kim Stanford,
Flemming Scheutz, Chad R. Laing, Linda Chui, Nicole Van De Kar, Patricia Griffin, Tim A....Biblioteca(s): INIA Las Brujas. |
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58. | | SCHILD, C.; BOABAID, F.M.; OLIVEIRA, L.G.S.; ARMENDANO, J.I.; SARAVIA, A.; CUSTODIO, A.; ALGORTA, J.; ALVAREZ, C.; JAURENA, M.; DIXON, R.M.; RIET-CORREA, F. Response of cows with osteomalacia grazing sub-tropical native pastures to phosphorus supplementation with loose mineral mix or feed blocks. Veterinary Journal. 2023, Volume 298-299, 106013. https://doi.org/10.1016/j.tvjl.2023.106013 Article history: Accepted 20 June 2023; Available online 22 June 2023. -- Correspondence author: Riet-Correa, F.; Programa de pós-graduação em Ciência Animal nos Trópicos, Escola de Medicina Veterinária e Zootecnia, Universidade Federal...Biblioteca(s): INIA Las Brujas. |
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Registros recuperados : 2,038 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
Fecha actual : |
24/10/2023 |
Actualizado : |
24/10/2023 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
Fecha de publicación : |
2023 |
Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
DOI : |
10.1111/imb.12875 |
Idioma : |
Inglés |
Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
Asunto categoría : |
L01 Ganadería |
URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
|
Marc : |
LEADER 02928naa a2200313 a 4500 001 1064356 005 2023-10-24 008 2023 bl uuuu u00u1 u #d 022 $a0962-1075 (print); 1365-2583 (electronic). 024 7 $a10.1111/imb.12875$2DOI 100 1 $aNOVAS, R. 245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource] 260 $c2023 500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- 520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. 653 $aCRISPR/Cas9 653 $aGenome editing 653 $aPartnership for the goals - Goal 17 653 $aPest control 653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA 653 $aScrewworm 653 $aSustainable Development Goals (SDGs) 653 $aU6 promoter 700 1 $aBASIKA, T. 700 1 $aWILLIAMSON, M. E. 700 1 $aFRESIA, P. 700 1 $aMENCHACA, A. 700 1 $aSCOTT, M. J. 773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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