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| 104. |  | RODRIGUES, G. D.; LUCAS, M.; ORTIZ, H. G.; SANTOS GONÇALVES, L. DOS; BLODORN, E.; DOMINGUES, W. B.; NUNES, L. S.; SARAVIA, A.; PARODI, P.; RIET-CORREA, F.; MENCHACA, A.; CAMPOS, C. V.; KROLOW, T. K.; KRÜGER, R. F. Molecular of Anaplasma marginale Theiler (Rickettsiales: Anaplasmataceae) in horseflies (Diptera: Tabanidae) in Uruguay. Scientific Reports, 2022, volume 12, issue 1, article 22460. OPEN ACCESS. doi: https://doi.org/10.1038/s41598-022-27067-0 Article history: Received 11 July 2022; Accepted 23 December 2022; Published 28 December 2022. -- Corresponding author: Rodrigues, G.D.; Ecology of Parasites and Vectors Group, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil;...| Biblioteca(s): INIA Las Brujas. |
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| 105. | | FERNÁNDEZ, E.A.; ROSALES, C.A.; GARZÓN, J. P.; ARGUDO, D.E.; AYALA, L.E.; GUEVARA, G.E.; MALDONADO, J.E.; PEREA, F.P. Morphological and histological characteristics of ovaries from two genetic groups of guinea pigs (Cavia porcellus) from South America. [Características morfológicas e histológicas de los ovarios de dos grupos genéticos de cuyes (Cavia porcellus) de América del Sur]. Sección: Artículos Primarios. Revista de Investigaciones Veterinarias del Perú, 2022, v. 33, n. 4, e23349. OPEN ACCESS. doi: https://doi.org/10.15381/rivep.v33i4.23349 Article history: Received January 11, 2022; Accepted for publication July 28, 2022; Published August 31, 2022. -- Publisher: Universidad Nacional Mayor de San Marcos. -- Correspondence author: Perea, F.P.; Facultad de Ciencias...| Biblioteca(s): INIA Las Brujas. |
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| 106. | | SEIXAS, J.N.; SANGIMINO, J.; GIANNITTI, F.; DORSCH, M.; CESAR, D.; RABENECK, D.M.; SILVA-FLANNERY, L.; FAIR, P.; RITTER, J.M. Identification of unexpected Mycobateria in feline and canine cutaneous lesions by PCR on formalin-fixed paraffin-embedded tissues. [abstract]. In: ACVP (Annual Meeting, American College of Veterinary Pathologists) and ASVCP (American Society for Veterinary Clinical Pathology) Annual Meeting, Boston, Massachusetts, USA. November 12-15, 2022.| Biblioteca(s): INIA Las Brujas. |
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| 107. | | MENEGASSI, S.R. DE O.; PEREIRA, G.R.; ROCHA, M.K.DA; KOETZ JR., C.; LOPEZ, L.F.D.; AUGUST, P.M.; MATTÉ, C.; GOMES, M.G.T.; DAL BEM, J. DE C.T.; CANOZZI, M.E.A.; BARCELLOS, J.O.J. Influence of tropical, subtropical, and temperate climate conditions on seminal redox status and sperm quality parameters in breeding bulls. Journal of Thermal Biology, October 2022, Volume 109, Article number 103319. doi: https://doi.org/10.1016/j.jtherbio.2022.103319 12 p.| Biblioteca(s): INIA Treinta y Tres. |
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| 110. | | LOZANO, J.; FERNÁNDEZ-CIGANDA, S.; GONZÁLEZ REVELLO, A.; HIRIGOYEN, D.; MARTÍNEZ, M.; SCORZA, C.; ZUNINO, P. Probiotic potential of GABA-producing lactobacilli isolated from Uruguayan artisanal cheese starter cultures. Journal of Microbiology, 2022, volume 133, issue 3, pages 1610-1619. doi: https://doi.org/10.1111/jam.15664 Article history: Received 18 March 2022; Revised 5 May 2022; Accepted 6 June 2022; Published online September 2022. -- Corresponding author: Zunino, P.; Department of Microbiology, Instituto de Investigaciones Biológicas Clemente Estable,...| Biblioteca(s): INIA La Estanzuela. |
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| 111. | | HECKER, Y. P.; BURUCÚA, M. M.; FIORANI, F.; MALDONADO RIVERA, J. E.; CIRONE, K.M.; DORSCH, M.; CHEUQUEPÁN, F. A.; CAMPERO, L. M.; CANTÓN, G. J.; MARÍN, M. S.; ORTEGA-MORA, L. M.; MOORE, D. P. Reactivation and foetal infection in pregnant heifers infected with Neospora caninum live tachyzoites at prepubertal age. Vaccines, 2022, Volume 10, Issue 8, article 1175. OPEN ACCESS. doi: https://doi.org/10.3390/vaccines10081175 Article history: Received 5 July 2022; Revised 14 July 2022; Accepted 22 July 2022; Published 25 July 2022.
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| 116. | | CASAUX, M.L.; COSTA, R.A. DA; MACÍAS-RIOSECO, M.; PERDOMO, T.; CAFFARENA, D.; SCHILD, C.; SILVEIRA, C.S.; ARÁOZ, V.; DONCEL, B.; FRAGA, M.; GIANNITTI, F. Salmonella enterica outbreaks in dairy calves in Uruguay . (AH-p24). [poster-Animal Health] Session Posters. In: World Buiatrics Congress (WBC), 31 st., Abstract Book, Madrid, Spain, September 4-8, 2022.| Biblioteca(s): INIA Las Brujas. |
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| 117. | | UZAL, F.A.; STOVER, S.; MORGAN, J.; FINO, C.; DIAB, S.; GIANNITTI, F.; HILL, A.E.; ARTHUR, R. Sudden death of racehorses in California: what is new?. [abstract] In: Proceedings of the American Association of Veterinary Laboratory Diagnosticians (AAVLD), 65th and United States Animal Health Association (USAHA) Annual Meeting, 126th, Minneapolis, Minnesota, USA. Oct. 6-12, 2022. pp.118. AAVLD 2022, 65th Annual Meeting "Technological Advances in Veterinary Diagnosis: what's here and what's on the horizon?".| Biblioteca(s): INIA Las Brujas. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
15/08/2022 |
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Actualizado : |
01/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
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Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
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Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
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DOI : |
10.1002/mrd.23638 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
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Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
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Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
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Asunto categoría : |
-- |
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Marc : |
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260 $c2022
500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.
520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.
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653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aPROTEINS
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773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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