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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/10/2023 |
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Actualizado : |
24/10/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
NOVAS, R.; BASIKA, T.; WILLIAMSON, M. E.; FRESIA, P.; MENCHACA, A.; SCOTT, M. J. |
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Afiliación : |
ROSSINA NOVAS, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; TATIANA BASIKA, Unidad Mixta entre Instituto Pasteur de Montevideo e INIA (UMPI), Montevideo, Uruguay; a Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; MEGAN E. WILLIAMSON, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MAXWELL J. SCOTT, Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, United States. |
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Título : |
Identification and functional analysis of Cochliomyia hominivorax U6 gene promoters. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Insect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS. |
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ISSN : |
0962-1075 (print); 1365-2583 (electronic). |
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DOI : |
10.1111/imb.12875 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. -- |
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Contenido : |
The New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society. |
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Palabras claves : |
CRISPR/Cas9; Genome editing; Partnership for the goals - Goal 17; Pest control; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA; Screwworm; Sustainable Development Goals (SDGs); U6 promoter. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
https://resjournals.onlinelibrary.wiley.com/doi/pdfdirect/10.1111/imb.12875
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Marc : |
LEADER 02928naa a2200313 a 4500
001 1064356
005 2023-10-24
008 2023 bl uuuu u00u1 u #d
022 $a0962-1075 (print); 1365-2583 (electronic).
024 7 $a10.1111/imb.12875$2DOI
100 1 $aNOVAS, R.
245 $aIdentification and functional analysis of Cochliomyia hominivorax U6 gene promoters.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 9 March 2023, Accepted 4 August 2023, First published 21 September 2023. -- Scott, M.J.; Department of Entomology and Plant Pathology, North Carolina State University, Campus Box 7613, Raleigh, NC, United States; email:mjscott3@ncsu.edu -- FUNDING: This research was supported by an agreement between The Institut Pasteur de Montevideo and North Carolina State University and grants from the Inter-American Development Bank (IBD UR-T1227) and from INIA (FTPA N°359). Tatiana Basika, Rossina Novas, Pablo Fresia and Alejo Menchaca are members of SNI (National Research System, Uruguay). -- Document type: Article Hybrid Gold Open Access. --
520 $aThe New World screwworm, Cochliomyia hominivorax, is an obligate parasite, which is a major pest of livestock. While the sterile insect technique was used very successfully to eradicate C. hominivorax from North and Central America, more cost-effective genetic methods will likely be needed in South America. The recent development of CRISPR/Cas9-based genetic approaches, such as homing gene drive, could provide a very efficient means for the suppression of C. hominivorax populations. One component of a drive system is the guide RNA(s) driven by a U6 gene promoter. Here, we have developed an in vivo assay to evaluate the activity of the promoters from seven C. hominivorax U6 genes. Embryos from the related blowfly Lucilia cuprina were injected with plasmid DNA containing a U6-promoter-guide RNA construct and a source of Cas9, either protein or plasmid DNA. Activity was assessed by the number of site-specific mutations in the targeted gene in hatched larvae. One promoter, Chom U6_b, showed the highest activity. These U6 gene promoters could be used to build CRISPR/Cas9-based genetic systems for the control of C. hominivorax. © 2023 The Authors. Insect Molecular Biology published by John Wiley & Sons Ltd on behalf of Royal Entomological Society.
653 $aCRISPR/Cas9
653 $aGenome editing
653 $aPartnership for the goals - Goal 17
653 $aPest control
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL - INIA
653 $aScrewworm
653 $aSustainable Development Goals (SDGs)
653 $aU6 promoter
700 1 $aBASIKA, T.
700 1 $aWILLIAMSON, M. E.
700 1 $aFRESIA, P.
700 1 $aMENCHACA, A.
700 1 $aSCOTT, M. J.
773 $tInsect Molecular Biology, 2023. Early View. https://doi.org/10.1111/imb.12875 -- OPEN ACCESS.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
14/01/2022 |
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Actualizado : |
14/01/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DINI, M.; RASEIRA, M. DO C. B.; UENO, B. |
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Afiliación : |
MAXIMILIANO ANTONIO DINI VIÑOLY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay // Universidade Federal de Pelotas, Faculdade de Agronomia Eliseu Maciel, Programa de Pós-Graduação em Agronomia, Pelotas, RS, Brazil.; MARIA DO CARMO BASSOLS RASEIRA, Embrapa Clima Temperado, Pelotas, Rio Grande do Sul, Brazil.; BERNARDO UENO, Embrapa Clima Temperado, Pelotas, Rio Grande do Sul, Brazil. |
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Título : |
Blossom blight resistance in peach: heritability and segregation in progenies from reciprocal crosses. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Revista Ceres (Viçosa) Nov. Dec. 2021, volume 68, número 6, pages 555-563. OPEN ACCESS. doi: https://doi.org/10.1590/0034-737X202168060007 |
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DOI : |
10.1590/0034-737X202168060007 |
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Idioma : |
Inglés |
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Notas : |
Article history: Submitted on September 23th, 2020 // Accepted on March 14th, 2021 // Published November 2021. |
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Contenido : |
Blossom blight and brown rot caused by Monilinia fructicola is the most important peach disease in Brazil. Genetic resistance is a control strategy that is gaining importance in breeding programs worldwide. This study aimed to identify genotypes with higher levels of blossom blight resistance to estimate the heritability of this character; study the frequency distribution in populations; and test the possibility of maternal effect. Blossom blight susceptibility was tested in reciprocal hybridizations seedlings, as well as their parents. The detached flower technique was used in a randomized complete block design, considering each genotype as a treatment. Flower inoculation was made by spraying a M. fructicola suspension and evaluations were carried out after 72 and 120 hours using a scale of five severity levels.
The studied populations presented low phenotypic variability regarding the flower resistance/susceptibility to M. fructicola, being most of them susceptible or very susceptible. Among the tested genotypes, the cultivars Maciel and Cerrito showed less blossom blight susceptibility, transmitting this character to their progenies. Heritability estimates of the blossom blight resistance were medium to low. The low heritability and its distribution in the progenies suggest that the character has additive inheritance, without detecting deviations associated with maternal effects. |
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Palabras claves : |
GENETIC RESISTANCE; Monilinia fructicola (Wint.) Honey; Prunus persica (L.) Batsch. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16183/1/Revista-Ceres-2021-Dini.pdf
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Marc : |
LEADER 02258naa a2200205 a 4500
001 1062636
005 2022-01-14
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1590/0034-737X202168060007$2DOI
100 1 $aDINI, M.
245 $aBlossom blight resistance in peach$bheritability and segregation in progenies from reciprocal crosses.$h[electronic resource]
260 $c2021
500 $aArticle history: Submitted on September 23th, 2020 // Accepted on March 14th, 2021 // Published November 2021.
520 $aBlossom blight and brown rot caused by Monilinia fructicola is the most important peach disease in Brazil. Genetic resistance is a control strategy that is gaining importance in breeding programs worldwide. This study aimed to identify genotypes with higher levels of blossom blight resistance to estimate the heritability of this character; study the frequency distribution in populations; and test the possibility of maternal effect. Blossom blight susceptibility was tested in reciprocal hybridizations seedlings, as well as their parents. The detached flower technique was used in a randomized complete block design, considering each genotype as a treatment. Flower inoculation was made by spraying a M. fructicola suspension and evaluations were carried out after 72 and 120 hours using a scale of five severity levels. The studied populations presented low phenotypic variability regarding the flower resistance/susceptibility to M. fructicola, being most of them susceptible or very susceptible. Among the tested genotypes, the cultivars Maciel and Cerrito showed less blossom blight susceptibility, transmitting this character to their progenies. Heritability estimates of the blossom blight resistance were medium to low. The low heritability and its distribution in the progenies suggest that the character has additive inheritance, without detecting deviations associated with maternal effects.
653 $aGENETIC RESISTANCE
653 $aMonilinia fructicola (Wint.) Honey
653 $aPrunus persica (L.) Batsch
700 1 $aRASEIRA, M. DO C. B.
700 1 $aUENO, B.
773 $tRevista Ceres (Viçosa) Nov. Dec. 2021, volume 68, número 6, pages 555-563. OPEN ACCESS. doi: https://doi.org/10.1590/0034-737X202168060007
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INIA Treinta y Tres (TT) |
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