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| 1. |  | GOLDBERG, V.; MACEDO, F.; CIAPPESONI, G. SNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock. Volume Electronic Poster Session - Genetic Gain - Genotyping & Phenotyping Strategies, , 359. In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359.| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 1 | |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
31/03/2021 |
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Actualizado : |
31/03/2021 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
GOLDBERG, V.; MACEDO, F.; CIAPPESONI, G. |
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Afiliación : |
VIRGINIA GOLDBERG BIANCHI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO LIBER MACEDO, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
SNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock. |
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Complemento del título : |
Volume Electronic Poster Session - Genetic Gain - Genotyping & Phenotyping Strategies, , 359. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359. |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignment was 21.8%. For Highlander, 7.5% of samples failed the genotyping and analyses was conducted without knowledge of the relationships between lambs with sires and dams. The 51% of the lambs genotyped had a sire and a dam assigned, 21% had only the sire assigned and 15% had only assigned a dam. Thus, genotyping by SNPs assigned a sire to the 72% of the lambs and a dam to the 66% of the lambs. The main problem was that only 5 of the 11 rams used as sires were genotyped and the high percentage of samples which failed the genotyping. However, taking into account the lack of information related to pedigree, we consider that a high rate of lambs had a sire and a dam assigned by the SNP panel. In conclusion, the development of a SNP panel for parentage assignment at a low price, would provide breeders with the opportunity of making mating management and control lambing easier and more relaxed while improving the known of pedigree information, ensuring high rates of genetic gain. Keywords: molecular markers, parentage exclusion, parentage verification, sheep. MenosABSTRACT.
Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignm... Presentar Todo |
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Palabras claves : |
Molecular markers; Parentage exclusion; Parentage verification; Sheep. |
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Thesagro : |
MARCADORES MOLECULARES; OVINOS. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15439/1/Goldberg-et-al.-2018.-WCGALP.pdf
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Marc : |
LEADER 03308nam a2200205 a 4500
001 1061916
005 2021-03-31
008 2018 bl uuuu u01u1 u #d
100 1 $aGOLDBERG, V.
245 $aSNP genotyping for parentage identification in a Merino nucleus and in a commercial Highlander flock.$h[electronic resource]
260 $aIn: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018, 359.$c2018
520 $aABSTRACT. Pedigree information is required to estimate breeding values accurately and to ensure high rates of genetic gain. DNA markers information can be used as an alternative to traditional recording of pedigree, being SNPs the markers of choice for parentage verification. The aim of the present study was to determine sheep parentage by SNPs using a very low density panel in: 1) a stud Merino flock to know the percentage of parentage error in order to correct pedigree misidentification and; 2) a commercial Highlander flock, to study the possibility to not control lambing anymore because for the breeder it is very laborious, time-consuming and disturb the relationship ewe-lambs during parturition. Genomic DNA was isolated from 200 samples of Merino sheep in 2015 and from 108 and 904 samples of Merino and Highlander sheep in 2016, respectively; and were genotyped with a very low density panel containing 507 SNPs. In 2015, for the 91 lambs genotyped, the error in parentage assignment was 16.5%. Although the assigned sire did not match with the declared sire for 15 lambs, the true sire was assigned for 14 of them. Thus, 99% of the lambs genotyped, had a sire assigned by the SNP panel. For the 80 lambs with their dams genotyped, the error rate was 12.5%. For the 10 lambs with mismatches with the declared dams, the true dam was assigned for five of them. In 2016, Merino samples were genotyped to link only lambs with their sires. For the 101 lambs, the error of parentage assignment was 21.8%. For Highlander, 7.5% of samples failed the genotyping and analyses was conducted without knowledge of the relationships between lambs with sires and dams. The 51% of the lambs genotyped had a sire and a dam assigned, 21% had only the sire assigned and 15% had only assigned a dam. Thus, genotyping by SNPs assigned a sire to the 72% of the lambs and a dam to the 66% of the lambs. The main problem was that only 5 of the 11 rams used as sires were genotyped and the high percentage of samples which failed the genotyping. However, taking into account the lack of information related to pedigree, we consider that a high rate of lambs had a sire and a dam assigned by the SNP panel. In conclusion, the development of a SNP panel for parentage assignment at a low price, would provide breeders with the opportunity of making mating management and control lambing easier and more relaxed while improving the known of pedigree information, ensuring high rates of genetic gain. Keywords: molecular markers, parentage exclusion, parentage verification, sheep.
650 $aMARCADORES MOLECULARES
650 $aOVINOS
653 $aMolecular markers
653 $aParentage exclusion
653 $aParentage verification
653 $aSheep
700 1 $aMACEDO, F.
700 1 $aCIAPPESONI, G.
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INIA Las Brujas (LB) |
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