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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02405naa a2200241 a 4500
001 1050705
005 2021-06-24
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
29/03/2021 |
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Actualizado : |
31/03/2021 |
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Tipo de producción científica : |
Trabajos en Congresos/Conferencias |
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Autor : |
NAVAJAS, E.; MACEDO, F.; LEMA, O.M.; LUZARDO, S.; AGUILAR, I. |
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Afiliación : |
ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO LIBER MACEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OSCAR MARIO LEMA QUEIJO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SANTIAGO FELIPE LUZARDO VILLAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; IGNACIO AGUILAR GARCIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Accuracy of genomic predictions for carcass and meat quality traits in the Uruguayan Hereford breed. |
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Complemento del título : |
Volume Species - Bovine (beef) 1, p. 636. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
In: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018. |
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Páginas : |
6 p. |
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Idioma : |
Inglés |
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Notas : |
Acknowledgements: This work was supported by the Agencia Nacional de Investigación e Innovación (ANII) (grants RTS_1_2012_1_3489 and FMV_1_2011_1_6671), Instituto Nacional de Investigación Agropecuaria (INIA), Sociedad de Criadores de Hereford (SCH), Instituto Nacional de Carnes (INAC) and Asociación Rural del Uruguay (ARU). |
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Contenido : |
SUMMARY.
Genomic selection provides the opportunity of genetic improvement of difficult-to-measure traits, such as carcass and meat traits. This study investigated the accuracies of genomic predictions of carcass and meat quality traits in Hereford with an initial training population of 747 steers. Hot carcass weights, rib eye areas and subcutaneous, fat depths were recorded in commercial slaughterhouses, as well as, the left pistola cut weight and retail product. Shear force and intramuscular fat content were measured in the Longissumus dorsi. |
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Palabras claves : |
Beef cattle; Carcass quality; Genomic selection; Meat quality; Predictability. |
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Asunto categoría : |
L01 Ganadería |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15399/1/accuracy-genomic-predictions-carcass-and-meat-quality-traits-uruguayan-hereford-breed.pdf
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Marc : |
LEADER 01665nam a2200241 a 4500
001 1061877
005 2021-03-31
008 2018 bl uuuu u01u1 u #d
100 1 $aNAVAJAS, E.
245 $aAccuracy of genomic predictions for carcass and meat quality traits in the Uruguayan Hereford breed.$h[electronic resource]
260 $aIn: Proceedings of the World Congress on Genetics Applied to Livestock Production, 11., Aotea Centre Auckland, New Zealand: WCGALP, ICAR, 11-16 feb 2018.$c2018
300 $a6 p.
500 $aAcknowledgements: This work was supported by the Agencia Nacional de Investigación e Innovación (ANII) (grants RTS_1_2012_1_3489 and FMV_1_2011_1_6671), Instituto Nacional de Investigación Agropecuaria (INIA), Sociedad de Criadores de Hereford (SCH), Instituto Nacional de Carnes (INAC) and Asociación Rural del Uruguay (ARU).
520 $aSUMMARY. Genomic selection provides the opportunity of genetic improvement of difficult-to-measure traits, such as carcass and meat traits. This study investigated the accuracies of genomic predictions of carcass and meat quality traits in Hereford with an initial training population of 747 steers. Hot carcass weights, rib eye areas and subcutaneous, fat depths were recorded in commercial slaughterhouses, as well as, the left pistola cut weight and retail product. Shear force and intramuscular fat content were measured in the Longissumus dorsi.
653 $aBeef cattle
653 $aCarcass quality
653 $aGenomic selection
653 $aMeat quality
653 $aPredictability
700 1 $aMACEDO, F.
700 1 $aLEMA, O.M.
700 1 $aLUZARDO, S.
700 1 $aAGUILAR, I.
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