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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
29/09/2014 |
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Actualizado : |
24/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; MARCONDES, J.A.; PICCHI, S.C.; STUCHI, E.S.; FADEL, A.L.; LAIA, M.L.; LEMOS, M.V.F. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Xylella fastidiosa: An in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis. |
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Fecha de publicación : |
2012 |
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Fuente / Imprenta : |
Electronic Journal of Biotechnology, 2012, v.15, no.3, p.1-33. |
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ISSN : |
0717-3458 |
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DOI : |
http://dx.doi.org/10.2225/vol15-issue3-fulltext-4 |
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Idioma : |
Inglés |
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Contenido : |
ABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels. MenosABSTRACT.
Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped... Presentar Todo |
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Thesagro : |
CITRUS; CYDIA. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02405naa a2200241 a 4500
001 1050705
005 2021-06-24
008 2012 bl uuuu u00u1 u #d
022 $a0717-3458
024 7 $ahttp://dx.doi.org/10.2225/vol15-issue3-fulltext-4$2DOI
100 1 $aFEDERICI, M.
245 $aXylella fastidiosa$bAn in vivo system to study possible survival strategies within citrus xylem vessels based on global gene expression analysis.$h[electronic resource]
260 $c2012
520 $aABSTRACT. Xylella fastidiosa inhabits the plant xylem, a nutrient-poor environment, so that mechanisms to sense and respond to adverse environmental conditions are extremely important for bacterial survival in the plant host. Although the complete genome sequences of different Xylella strains have been determined, little is known about stress responses and gene regulation in these organisms. In this work, a DNA microarray was constructed containing 2,600 ORFs identified in the genome sequencing project of Xylella fastidiosa 9a5c strain, and used to check global gene expression differences in the bacteria when it is infecting a symptomatic and a tolerant citrus tree. Different patterns of expression were found in each variety, suggesting that bacteria are responding differentially according to each plant xylem environment. The global gene expression profile was determined and several genes related to bacterial survival in stressed conditions were found to be differentially expressed between varieties, suggesting the involvement of different strategies for adaptation to the environment. The expression pattern of some genes related to the heat shock response, toxin and detoxification processes, adaptation to atypical conditions, repair systems as well as some regulatory genes are discussed in this paper. DNA microarray proved to be a powerful technique for global transcriptome analyses. This is one of the first studies of Xylella fastidiosa gene expression in vivo which helped to increase insight into stress responses and possible bacterial survival mechanisms in the nutrient-poor environment of xylem vessels.
650 $aCITRUS
650 $aCYDIA
700 1 $aMARCONDES, J.A.
700 1 $aPICCHI, S.C.
700 1 $aSTUCHI, E.S.
700 1 $aFADEL, A.L.
700 1 $aLAIA, M.L.
700 1 $aLEMOS, M.V.F.
773 $tElectronic Journal of Biotechnology, 2012$gv.15, no.3, p.1-33.
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
10/03/2021 |
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Actualizado : |
10/03/2021 |
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Tipo de producción científica : |
Artículos Indexados |
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Autor : |
DINI, M.; SCARIOTTO, S.; MARCHI, P.M.; RASEIRA, M. DO C. B.; SCHIAVON, A.V. |
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Afiliación : |
MAXIMILIANO ANTONIO DINI VIÑOLY, Universidade Federal de Pelotas (UFPel); SILVIA SCARIOTTO, Embrapa Clima Temperado; PRISCILA MONALISA MARCHI, Universidade Federal de Pelotas (UFPel); MARIA DO CARMO BASSOLS RASEIRA, Embrapa Clima Temperado; ANDRESSA VIGHI SCHIAVON, Universidade Federal de Pelotas (UFPel). |
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Título : |
Segregation of thornlessness in blackberry progenies. [Segregaçâo para ausência de espinhos em progenies de amoreira-preta.] |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Revista da 14ª Jornada de Pós-Graduaçâo e Pesquisa-Congrega Urcamp, 2017, p.2548-2560. |
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ISSN : |
2526-4397 |
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Idioma : |
Portugués |
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Notas : |
Article history: Submetido: 09 setembro 2017; Avaliado: 27 setembro 2017. |
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Contenido : |
RESUMO - A presença de espinhos nas hastes e folhas das plantas de amoreira-preta dificulta o manejo cultural, como a poda e a colheita. O desenvolvimento de
cultivares sem espinhos tem sido um dos principais objetivos dos programas de melhoramento desta cultura no mundo. Este trabalho teve como objetivo testar se há
correlação entre a densidade e tamanho de espinhos dos genitores com a frequência de indivíduos sem espinhos nas suas progênies; além de comparar os
genitores quanto ao número e tamanho de espinhos e testar a correlação entre esses caracteres. O trabalho foi realizado na Embrapa Clima Temperado, Pelotas,
RS, nos anos 2016 e 2017. Foram utilizadas doze progênies obtidas por autofecundação das seleções Black 132, Black 141, Black 183, Black 190, Black
219, Black 229, Black 230, Black 241, Black 274, Black 275, Seleção 21/96 e Seleção 22/96. Para isso, foram ensacadas flores, em estádio de balão, de cada
seleção. As sementes extraídas foram escarificadas em ácido sulfúrico e acondicionadas sobre papel filtro embebido em solução com fungicida, e em seguida
foram embaladas em sacos plásticos e colocadas em câmara fria (4±1°C) até ser observada a emissão dos primórdios radiculares. As sementes foram então,
transferidas para caixas de germinação. Quando as plântulas apresentavam os cotilédones totalmente visíveis, avaliou-se em lupa binocular a presença ou ausência
de pelos glandulares nos cotilédones de cada indivíduo das progênies. Também foram analisadas a densidade e tamanho dos espinhos em cinco clones de cada
seleção utilizadas como genitores, e os dados foram submetidos à análise da variância e ao teste de agrupamento de Scott-Knott. As variáveis mensuradas, tanto
nas seleções quanto a frequência de indivíduos sem espinhos nas progênies, foram submetidas à correlação linear de Pearson. Os caracteres de densidade e tamanho
de espinhos dos genitores não estão correlacionados com a frequência de indivíduos sem espinhos nas suas progênies. Nas seleções utilizadas como genitoras existe ampla variabilidade quanto ao número e tamanho de espinhos. Os caracteres número, comprimento, largura e espessura de espinhos entre as hastes primárias e secundárias estão altamente correlacionados, não existindo necessidade de avaliar separadamente os dois tipos de brotações.
.-.-.-.-.-..
ABSTRACT - The presence of thorns on the canes and leaves of blackberry plants hinders cultural management such as pruning and harvesting. The development of
thornless cultivars has been one of the main objectives of blackberry breeding programsall over the world. The objective of this work was to test if there is a
correlation between the density and size of thorns of the parents with the frequency of thornless individuals in their progenies. In addition, comparisons between thorn
number and size in the selections as well as correlations between these characters were performed. The work was carried out at Embrapa Clima Temperado, Pelotas,
RS, on the years 2016 and 2017. Twelve progenies originated through self pollination of selections Black 132, Black 141, Black 183, Black 190, Black 219, Black 229,
Black 230, Black 241, Black 274, Black 275, Seleção 21/96 and Seleção 22/96 were used. Flowers of these selections were bagged at balloon stage selection, to avoid
cross-pollination. The fruits of each selection were collected and the extracted seeds were scarified in sulfuric acid and packed in plastic bags with filter paper soaked in
fungicide solution. The bags were placed in a cold chamber (4±1°C) until the emission of the roots was observed. After this period, the seeds were transferred to
germination boxes. When their cotyledons were fully visible, the presence or absence of glandular hairs on their boders was observed under a stereo microscope. The
thorn´s density and size were also analyzed in five clones of each selection used as parents, and the data were submitted to variance analysis and Scott-Knott's test. The evaluated were submitted to Pearson's linear correlation test. The density and size of thorns of the parents are not correlated with the frequency of thornless individuals in their progeny. There is wide variability as to the number and size of thorns, among selections used as parents, in this work. The number, height, length and width of spines between the primary and secondary canes are highly correlated, and there is no need to evaluate separately the two types of shoots. MenosRESUMO - A presença de espinhos nas hastes e folhas das plantas de amoreira-preta dificulta o manejo cultural, como a poda e a colheita. O desenvolvimento de
cultivares sem espinhos tem sido um dos principais objetivos dos programas de melhoramento desta cultura no mundo. Este trabalho teve como objetivo testar se há
correlação entre a densidade e tamanho de espinhos dos genitores com a frequência de indivíduos sem espinhos nas suas progênies; além de comparar os
genitores quanto ao número e tamanho de espinhos e testar a correlação entre esses caracteres. O trabalho foi realizado na Embrapa Clima Temperado, Pelotas,
RS, nos anos 2016 e 2017. Foram utilizadas doze progênies obtidas por autofecundação das seleções Black 132, Black 141, Black 183, Black 190, Black
219, Black 229, Black 230, Black 241, Black 274, Black 275, Seleção 21/96 e Seleção 22/96. Para isso, foram ensacadas flores, em estádio de balão, de cada
seleção. As sementes extraídas foram escarificadas em ácido sulfúrico e acondicionadas sobre papel filtro embebido em solução com fungicida, e em seguida
foram embaladas em sacos plásticos e colocadas em câmara fria (4±1°C) até ser observada a emissão dos primórdios radiculares. As sementes foram então,
transferidas para caixas de germinação. Quando as plântulas apresentavam os cotilédones totalmente visíveis, avaliou-se em lupa binocular a presença ou ausência
de pelos glandulares nos cotilédones de cada indivíduo das progênies. Também foram analisadas a densidade... Presentar Todo |
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Palabras claves : |
Berries; Fruit breeding; Melhoramento genético; Pequenas frutas; Rubus spp. |
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Thesagro : |
ZARZAMORA. |
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Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15321/1/Dini-et-al.-2017.-Segregation-of-thornlessness-in-blackberry-progenies-Congrega.pdf
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Marc : |
LEADER 05413naa a2200265 a 4500
001 1061815
005 2021-03-10
008 2017 bl uuuu u00u1 u #d
022 $a2526-4397
100 1 $aDINI, M.
245 $aSegregation of thornlessness in blackberry progenies. [Segregaçâo para ausência de espinhos em progenies de amoreira-preta.]$h[electronic resource]
260 $c2017
500 $aArticle history: Submetido: 09 setembro 2017; Avaliado: 27 setembro 2017.
520 $aRESUMO - A presença de espinhos nas hastes e folhas das plantas de amoreira-preta dificulta o manejo cultural, como a poda e a colheita. O desenvolvimento de cultivares sem espinhos tem sido um dos principais objetivos dos programas de melhoramento desta cultura no mundo. Este trabalho teve como objetivo testar se há correlação entre a densidade e tamanho de espinhos dos genitores com a frequência de indivíduos sem espinhos nas suas progênies; além de comparar os genitores quanto ao número e tamanho de espinhos e testar a correlação entre esses caracteres. O trabalho foi realizado na Embrapa Clima Temperado, Pelotas, RS, nos anos 2016 e 2017. Foram utilizadas doze progênies obtidas por autofecundação das seleções Black 132, Black 141, Black 183, Black 190, Black 219, Black 229, Black 230, Black 241, Black 274, Black 275, Seleção 21/96 e Seleção 22/96. Para isso, foram ensacadas flores, em estádio de balão, de cada seleção. As sementes extraídas foram escarificadas em ácido sulfúrico e acondicionadas sobre papel filtro embebido em solução com fungicida, e em seguida foram embaladas em sacos plásticos e colocadas em câmara fria (4±1°C) até ser observada a emissão dos primórdios radiculares. As sementes foram então, transferidas para caixas de germinação. Quando as plântulas apresentavam os cotilédones totalmente visíveis, avaliou-se em lupa binocular a presença ou ausência de pelos glandulares nos cotilédones de cada indivíduo das progênies. Também foram analisadas a densidade e tamanho dos espinhos em cinco clones de cada seleção utilizadas como genitores, e os dados foram submetidos à análise da variância e ao teste de agrupamento de Scott-Knott. As variáveis mensuradas, tanto nas seleções quanto a frequência de indivíduos sem espinhos nas progênies, foram submetidas à correlação linear de Pearson. Os caracteres de densidade e tamanho de espinhos dos genitores não estão correlacionados com a frequência de indivíduos sem espinhos nas suas progênies. Nas seleções utilizadas como genitoras existe ampla variabilidade quanto ao número e tamanho de espinhos. Os caracteres número, comprimento, largura e espessura de espinhos entre as hastes primárias e secundárias estão altamente correlacionados, não existindo necessidade de avaliar separadamente os dois tipos de brotações. .-.-.-.-.-.. ABSTRACT - The presence of thorns on the canes and leaves of blackberry plants hinders cultural management such as pruning and harvesting. The development of thornless cultivars has been one of the main objectives of blackberry breeding programsall over the world. The objective of this work was to test if there is a correlation between the density and size of thorns of the parents with the frequency of thornless individuals in their progenies. In addition, comparisons between thorn number and size in the selections as well as correlations between these characters were performed. The work was carried out at Embrapa Clima Temperado, Pelotas, RS, on the years 2016 and 2017. Twelve progenies originated through self pollination of selections Black 132, Black 141, Black 183, Black 190, Black 219, Black 229, Black 230, Black 241, Black 274, Black 275, Seleção 21/96 and Seleção 22/96 were used. Flowers of these selections were bagged at balloon stage selection, to avoid cross-pollination. The fruits of each selection were collected and the extracted seeds were scarified in sulfuric acid and packed in plastic bags with filter paper soaked in fungicide solution. The bags were placed in a cold chamber (4±1°C) until the emission of the roots was observed. After this period, the seeds were transferred to germination boxes. When their cotyledons were fully visible, the presence or absence of glandular hairs on their boders was observed under a stereo microscope. The thorn´s density and size were also analyzed in five clones of each selection used as parents, and the data were submitted to variance analysis and Scott-Knott's test. The evaluated were submitted to Pearson's linear correlation test. The density and size of thorns of the parents are not correlated with the frequency of thornless individuals in their progeny. There is wide variability as to the number and size of thorns, among selections used as parents, in this work. The number, height, length and width of spines between the primary and secondary canes are highly correlated, and there is no need to evaluate separately the two types of shoots.
650 $aZARZAMORA
653 $aBerries
653 $aFruit breeding
653 $aMelhoramento genético
653 $aPequenas frutas
653 $aRubus spp
700 1 $aSCARIOTTO, S.
700 1 $aMARCHI, P.M.
700 1 $aRASEIRA, M. DO C. B.
700 1 $aSCHIAVON, A.V.
773 $tRevista da 14ª Jornada de Pós-Graduaçâo e Pesquisa-Congrega Urcamp, 2017, p.2548-2560.
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