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| 1. |  | DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy| Biblioteca(s): INIA Treinta y Tres. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
22/01/2021 |
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Actualizado : |
14/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
DELPIAZZO, R.; BARCELLOS, M.; BARROS, S.; BENTANCOR, L.; FRAGA, M.; GIL, J.; IRAOLA, G.; MORSELLA, C.; PAOLICCHI, F.; PÉREZ, R.; RIET-CORREA, F.; SANGUINETTI, M.; SILVA, A.; SILVEIRA, C.S.; CALLEROS, L. |
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Afiliación : |
RAFAEL DELPIAZZO, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; MAILA BARCELLOS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; SOFÍA BARROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; LAURA BENTANCOR, Universidad de la República Oriental del Uruguay. Facultad de Medicina. Instituto de Higiene. Departamento de Bacteriología y Virología. Montevideo, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JORGE GIL, Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni". Departamento de Salud de los Sistemas Pecuarios. Paysandú, Uruguay.; GREGORIO IRAOLA, Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana, Montevideo, Uruguay. / Universidad Mayor. Facultad de Ciencias. Centro de Biología Integrativa. Santiago de Chile, Chile.; CLAUDIA MORSELLA, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; FERNANDO PAOLICCHI, Estación Experimental INTA Balcarce. Laboratorio de Bacteriología. Balcarce, Buenos Aires, Argentina.; RUBEN PEREZ, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; ALFONSO SILVA, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay.; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUCÍA CALLEROS, Universidad de la República Oriental del Uruguay. Facultad de Ciencias. Sección Genética Evolutiva. Montevideo, Uruguay. |
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Título : |
Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Veterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163 |
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DOI : |
10.1016/j.vas.2020.100163 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020.
Corresponding author: laurabet@higiene.edu.uy |
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Contenido : |
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity. |
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Palabras claves : |
BOVINE GENITAL CAMPYLOBACTERIOSIS; CAMPYLOBACTER FETUS; MOLECULAR DIAGNOSIS; MOLECULAR DIAGNOSTICS; QPCR. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14934/1/Veterinary-Animal-Science-2021-100163.pdf
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Marc : |
LEADER 02681naa a2200373 a 4500
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024 7 $a10.1016/j.vas.2020.100163$2DOI
100 1 $aDELPIAZZO, R.
245 $aAccurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 21 October 2020 / Received in revised form 20 December 2020 / Accepted 22 December 2020 / available online 24 December 2020. Corresponding author: laurabet@higiene.edu.uy
520 $aCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow?s medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%?100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
653 $aBOVINE GENITAL CAMPYLOBACTERIOSIS
653 $aCAMPYLOBACTER FETUS
653 $aMOLECULAR DIAGNOSIS
653 $aMOLECULAR DIAGNOSTICS
653 $aQPCR
700 1 $aBARCELLOS, M.
700 1 $aBARROS, S.
700 1 $aBENTANCOR, L.
700 1 $aFRAGA, M.
700 1 $aGIL, J.
700 1 $aIRAOLA, G.
700 1 $aMORSELLA, C.
700 1 $aPAOLICCHI, F.
700 1 $aPÉREZ, R.
700 1 $aRIET-CORREA, F.
700 1 $aSANGUINETTI, M.
700 1 $aSILVA, A.
700 1 $aSILVEIRA, C.S.
700 1 $aCALLEROS, L.
773 $tVeterinary and Animal Science, January 2021, vol.11 no. 100165, 5 p. OPEN ACCESS. Doi: https://doi.org/10.1016/j.vas.2020.100163
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