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| 1. |  | CAFFARENA, D.; MEIRELES, M.V; CARRASCO-LETELIER, L.; PICASSO-RISSO, C.; SANTANA ,BN; RIET-CORREA, F.; GIANNITTI, F. Dairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination. Frontiers in Veterinary Science, July 2020, v.7, 562. OPEN ACCESS. DOI:https://doi.org/10.3389/fvets.2020.00562 Article history: Received: 20 March 2020/Accepted: 15 July 2020/Published: 21 August 2020.*Correspondence: Rubén Darío Caffarena
(dcaffarena@inia.org.uy) Federico Giannitti (fgiannitti@inia.org.uy). This work was partially funded by...| Biblioteca(s): INIA La Estanzuela. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
04/09/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CAFFARENA, D.; MEIRELES, M.V; CARRASCO-LETELIER, L.; PICASSO-RISSO, C.; SANTANA ,BN; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay; MARCELO VASCONCELOS MEIRELES, São Paulo State University Department Faculdade de Medicina Veterinária de Araçatuba, Brasil.; LEONIDAS CARRASCO-LETELIER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CATALINA PICASSO-RISSO, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay./Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, United States.; BRUNA NICOLETI SANTANA, Faculdade de Medicina Veterinária, Universidade Estadual Paulista, Araçatuba, Brazil.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Dairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Veterinary Science, July 2020, v.7, 562. OPEN ACCESS. DOI:https://doi.org/10.3389/fvets.2020.00562 |
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DOI : |
10.3389/fvets.2020.00562 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 20 March 2020/Accepted: 15 July 2020/Published: 21 August 2020.*Correspondence: Rubén Darío Caffarena
(dcaffarena@inia.org.uy) Federico Giannitti (fgiannitti@inia.org.uy). This work was partially funded by grants FMV_1_2014_1_104922, of the Uruguayan Agencia Nacional de Investigación e Innovación (ANII), and grants N-15156_PL_15_0_00 and N-23398 of the Instituto
Nacional de Investigación Agropecuaria (INIA). RC acknowledges support from ANII through a master?s scholarship (POS_FMV_2015_1_1005180). |
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Contenido : |
Abstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay?s capital city and its main water potabilization plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20?900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water potabilization plants located 9?108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk. MenosAbstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have ... Presentar Todo |
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Palabras claves : |
BOVINE CRYPTOSPORIDIOSIS,; CRYPTOSPORIDIUM PARVUM ZOONOTIC SUBTYPES; DAIRY CALVES; DIARRHEA; PLATAFORMA DE SALUD ANIMAL; SPATIAL CLUSTERS; SURFACE WATER; TERNEROS LECHEROS. |
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Thesagro : |
URUGUAY. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16689/1/fvets-07-00562.pdf
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7473207/pdf/fvets-07-00562.pdf
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Marc : |
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100 1 $aCAFFARENA, D.
245 $aDairy calves in Uruguay are reservoirs of zoonotic subtypes of cryptosporidium parvum and Pose a potential risk of surface water contamination.$h[electronic resource]
260 $c2020
500 $aArticle history: Received: 20 March 2020/Accepted: 15 July 2020/Published: 21 August 2020.*Correspondence: Rubén Darío Caffarena (dcaffarena@inia.org.uy) Federico Giannitti (fgiannitti@inia.org.uy). This work was partially funded by grants FMV_1_2014_1_104922, of the Uruguayan Agencia Nacional de Investigación e Innovación (ANII), and grants N-15156_PL_15_0_00 and N-23398 of the Instituto Nacional de Investigación Agropecuaria (INIA). RC acknowledges support from ANII through a master?s scholarship (POS_FMV_2015_1_1005180).
520 $aAbstract:Cryptosporidium parvum, a major cause of diarrhea in calves, is of concern given its zoonotic potential. Numerous outbreaks of human cryptosporidiosis caused by C. parvum genetic subtypes are reported yearly worldwide, with livestock or water being frequently identified sources of infection. Although cryptosporidiosis has been reported from human patients in Uruguay, particularly children, epidemiologic information is scant and the role of cattle as reservoirs of zoonotic subtypes of C. parvum has not been explored. In this study, we aimed to (a)-identify C. parvum subtypes infecting dairy calves in Uruguay (including potentially zoonotic subtypes), (b)-assess their association with calf diarrhea, (c)-evaluate their spatial clustering, and (d)-assess the distance of infected calves to surface watercourses draining the farmlands and determine whether these watercourses flow into public water potabilization plants. Feces of 255 calves that had tested positive for Cryptosporidium spp. by antigen ELISA were selected. Samples had been collected from 29 dairy farms in seven Uruguayan departments where dairy farming is concentrated and represented 170 diarrheic and 85 non-diarrheic calves. Selected samples were processed by nested PCRs targeting the 18S rRNA and gp60 genes followed by sequencing to identify C. parvum subtypes. Of seven C. parvum subtypes detected in 166 calves, five (identified in 143 calves on 28/29 farms) had been identified in humans elsewhere and have zoonotic potential. Subtype IIaA15G2R1 was the most frequent (53.6%; 89/166), followed by IIaA20G1R1 (24.1%; 40/166), IIaA22G1R1 (11.4%; 19/166), IIaA23G1R1 (3.6%; 6/166), IIaA17G2R1 (3%; 5/166), IIaA21G1R1 (2.4%; 4/166), and IIaA16G1R1 (1.8%; 3/166). There were no significant differences in the proportions of diarrheic and non-diarrheic calves infected with any of the C. parvum subtypes. Two spatial clusters were detected, one of which overlapped with Uruguay?s capital city and its main water potabilization plant (Aguas Corrientes), harvesting surface water to supply ~1,700,000 people. Infected calves on all farms were within 20?900 m of a natural surface watercourse draining the farmland, 10 of which flowed into six water potabilization plants located 9?108 km downstream. Four watercourses flowed downstream into Aguas Corrientes. Calves are reservoirs of zoonotic C. parvum subtypes in Uruguay and pose a public health risk.
650 $aURUGUAY
653 $aBOVINE CRYPTOSPORIDIOSIS,
653 $aCRYPTOSPORIDIUM PARVUM ZOONOTIC SUBTYPES
653 $aDAIRY CALVES
653 $aDIARRHEA
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aSPATIAL CLUSTERS
653 $aSURFACE WATER
653 $aTERNEROS LECHEROS
700 1 $aMEIRELES, M.V
700 1 $aCARRASCO-LETELIER, L.
700 1 $aPICASSO-RISSO, C.
700 1 $aSANTANA ,BN
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tFrontiers in Veterinary Science, July 2020$gv.7, 562. OPEN ACCESS. DOI:https://doi.org/10.3389/fvets.2020.00562
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