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| Registros recuperados : 10 | |
| 1. |  | BIANCO, C.; ROMERO, A.; BRANDA, A.; FEDERICI, M.; DALLA RIZZA, M.; LLAMBI, S.; GIANNITTI, F.; CAFFARENA, D.; SCHILD, C.; CASAUX, M.L.; DUTRA, F. Mutaciones letales y semiletales en terneros Holando de Uruguay. In: Jornadas Uruguayas de Buiatría, 47., 2019, Paysandú, UY.; Matto, ; Gianeechini, E. (Ed.). Paysandú: Centro Médico Veterinario de Paysandú/Sociedad Uruguaya de Buiatría, 2019. p.114-117.| Biblioteca(s): INIA La Estanzuela. |
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| 2. | | BRANDA, A.; FEDERICI, M.; BRIANO, C.; ROMERO, A.; LLAMBÍ, S.; DUTRA, F.; DALLA RIZZA, M. Avances en la identificación de portadores de la deficiencia en la adhesión leucocitaria bovina en raza Holando. In: JORNADA TÉCNICA, VIII JORNADA DE AGROBIOTECNOLOGÍA. INIA LAS BRUJAS, 30 DE OCTUBRE DE 2014. UNIDAD DE BIOTECNOLOGÍA. Montevideo (Uruguay): INIA, 2014. 7-9 (Actividades de Difusión; 741)| Biblioteca(s): INIA Las Brujas. |
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| 3. | | BRANDA, A.; FEDERICI, M.; DUTRA, F.; ROMERO, A.; BRIANO, C.; DALLA RIZZA, M.; LLAMBÍ, S. Identificación de terneras Holando portadoras de BLAD y Citrulinemia en la región Este de Uruguay por PCR-RFLP y secuenciación. (Identification of Holstein heifer?s carriers BLAD and Citrullinemia in the eastern region of Uruguay by PCR-RFLP and sequencing). Veterinaria (Montevideo), 2016, v. 52, no. 202, p. 23-27. Recibido :11/5/2015. Aceptado: 28/8/2015| Biblioteca(s): INIA Las Brujas. |
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| 5. | | BRANDA, A.; ROMERO, A.; FEDERICI, M.; BRIANO, C.; DALLA RIZZA, M.; DUTRA, F. Identificación de vacas Polled Hereford portadoras de Msud C248>T mediante PCR-HR, y confirmación por secuenciación. GGM 25 - COMUNICACIONES LIBRES - GGM. GENÓMICA Y GENÉTICA MOLECULAR In: JOURNAL OF BASIC & APPLIED GENETICS, 2016, Vol.27, Iss. 1 (Supp.). XVI LATIN AMERICAN CONGRESS OF GENETICS, IV CONGRESS OF THE URUGUAYAN SOCIETY OF GENETICS, XLIX ANNUAL MEETING OF THE GENETICS SOCIETY OF CHILE, XLV ARGENTINE CONGRESS OF GENETICS, 9-12 October 2016. PROCEEDINGS. Montevideo (Uruguay): SAG, 2016. p. 267| Biblioteca(s): INIA Las Brujas. |
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| 6. | | BRANDA-SICA, A.; FEDERICI, M.; DUTRA, F.; BRIANO, C.; ROMERO, A.; DALLA RIZZA, M.; LLAMBÍ, S. Genotipado de SNPs relacionados con enfermedades hereditarias en terneras Holando utilizando el panel GGP-BovineLDv3. [Genotyping of SNPs related to hereditary diseases in Holstein heifers using the GGP-BovineLDv3 panel.] Sección: Artículos originales. Veterinaria (Montevideo), 2018, v. 54, no. 210, p. 5-10. -- OPEN ACCESS. Artilce history: Recibido 20 Diciembre 2017; Aceptado 20 Agosto 2018; Publicado 1 Noviembre 2018. -- Correspondencia: A. Branda Sica: abranda@inia.org.uy -- Correspondencia: MT. Federici: mfederici@inia.org.uy -- Publicación de la...| Biblioteca(s): INIA Las Brujas. |
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| 7. | | BRIANO, C.; REGIDOR-CERRILLO, J.; EASTON, C.; PIERUCCIONI, F.; ROMERO, A.; PERAZA, P.; MEDEROS, A.; DUTRA, F. Diagnóstico molecular de Neospora caninum en fetos abortados espontáneamente en bovinos de Uruguay. [Molecular diagnosis of Neospora caninum in aborted bovine fetus from Uruguay.]. Sección: Artículos originales. Veterinaria (Montevideo), 2021, v.57(216), e20215721603. doi: https://doi.org/10.29155/VET.57.216.3 Article history: Recibido 16 jul 2020; Aceptado 01 jul 2021; Publicado 29 set 2021. -- Autor para correspondencia: Carolina Briano, e-mail: cbriano@mgap.gub.uy -- Publicación de la Sociedad de Medicina Veterinaria del Uruguay (SMVU).| Biblioteca(s): INIA Las Brujas. |
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| 8. | | BRIANO, C.; ROMERO, A.; BRANDA, A.; FEDERICI, M.; DALLA RIZZA, M.; LLAMBÍ, S.; GIANNITTI, F.; CAFFARENA, D.; SCHILD, C.; CASAUX, M.L.; DUTRA, F. Principales mutaciones letales y semiletales en terneros Holando de Uruguay. [resumen] In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, XI. Encuentro Nacional de REDBIO, III. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2018. p.6-7 (Serie Actividades de Difusión; 786)| Biblioteca(s): INIA Las Brujas. |
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| 9. | | BRIANO-RODRÍGUEZ, C.; ROMERO, A.; LLAMBÍ, S.; BRANDA, A.; FEDERICI, M.; GIANNITTI, F.; CAFFARENA, D.; SCHILD, C.; CASAUX, M.L.; DUTRA, F. Lethal and semi-lethal mutations in Holstein calves in Uruguay. [Mutações letais e semi-letais em bezerros da raça Holandesa no Uruguai.] Animal Production. Ciencia Rural, 2021, Volume 51, Issue 7, Article number e20200734. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200734 Article history: Received: August 07, 2020; Accepted: November 23, 2020; Revised: February 23, 2021.
This research was funded by grant N-15156 of the uruguayan Instituto Nacional de Investigación Agropecuaria (INIA).| Biblioteca(s): INIA Las Brujas. |
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| Registros recuperados : 10 | |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
04/03/2020 |
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Actualizado : |
05/09/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
MENA, E.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
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Afiliación : |
EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCOS MONTESANO, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay.; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Soybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733 |
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DOI : |
10.3389/fpls.2019.01733 |
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Idioma : |
Inglés |
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Notas : |
Article history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020.
The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826). |
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Contenido : |
Abstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses. MenosAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xyl... Presentar Todo |
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Palabras claves : |
CELL WALL; DEFENSE GENES; DIAPORTHE CAULIVORA; DISEASE SYMPTOMS; INTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA); PATHOGEN COLONIZATION; SOYBEAN STEM CANKER; TRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a). |
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Thesagro : |
ENFERMEDADES DE LAS PLANTAS; PATÓGENOS; SOJA. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16699/1/fpls-10-01733.pdf
https://www.frontiersin.org/articles/10.3389/fpls.2019.01733/pdf
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Marc : |
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005 2022-09-05
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2019.01733$2DOI
100 1 $aMENA, E.
245 $aSoybean stem canker caused by diaporthe caulivora; pathogen diversity, colonization process, and plant defense activation.$h[electronic resource]
260 $c2020
500 $aArticle history:Received: 20 May 2019.//Accepted: 09 December 2019.// Published: 23 January 2020. The authors thank Ricardo Larraya for technical assistance and Andrés Di Paolo for assistance in confocal microscopy analysis.This work was supported by Agencia Nacional de Investigación e Innovación (ANII) (grant RTS-1-2014, and graduate fellowships), and Programa de Desarrollo de las Ciencias Básicas (PEDECIBA) Uruguay.The datasets generated for this study can be found in the GeneBank database (MK483139-MK483213, MK507892, and MN584748-MN584826).
520 $aAbstract:Soybean is an important crop in South America, and its production is limited by fungal diseases caused by species from the genus Diaporthe, including seed decay, pod and stem blight, and soybean stem canker (SSC). In this study, we focused on Diaporthe species isolated from soybean plants with SSC lesions in different parts of Uruguay. Diaporthe diversity was determined by sequencing the internal transcribed spacer (ITS) regions of ribosomal RNA and a partial region of the translation elongation factor 1-alpha gene (TEF1?). Phylogenetic analysis showed that the isolates belong to five defined groups of Diaporthe species, Diaporthe caulivora and Diaporthe longicolla being the most predominant species present in stem canker lesions. Due to the importance of D. caulivora as the causal agent of SSC in the region and other parts of the world, we further characterized the interaction of this pathogen with soybean. Based on genetic diversity of D. caulivora isolates evaluated with inter-sequence single repetition (ISSR), three different isolates were selected for pathogenicity assays. Differences in virulence were observed among the selected D. caulivora isolates on susceptible soybean plants. Further inspection of the infection and colonization process showed that D. caulivora hyphae are associated with trichomes in petioles, leaves, and stems, acting probably as physical adhesion sites of the hyphae. D. caulivora colonized the stem rapidly reaching the phloem and the xylem at 72 h post-inoculation (hpi), and after 96 hpi, the stem was heavily colonized. Infected soybean plants induce reinforcement of the cell walls, evidenced by incorporation of phenolic compounds. In addition, several defense genes were induced in D. caulivora?inoculated stems, including those encoding a pathogenesis-related protein-1 (PR-1), a PR-10, a ?-1,3-glucanase, two chitinases, two lipoxygenases, a basic peroxidase, a defensin, a phenylalanine-ammonia lyase, and a chalcone synthase. This study provides new insights into the interaction of soybean with D. caulivora, an important pathogen causing SSC, and provides information on the activation of plant defense responses.
650 $aENFERMEDADES DE LAS PLANTAS
650 $aPATÓGENOS
650 $aSOJA
653 $aCELL WALL
653 $aDEFENSE GENES
653 $aDIAPORTHE CAULIVORA
653 $aDISEASE SYMPTOMS
653 $aINTERNAL TRANSCRIBED SPACER (ITS) RIBOSOMAL RNA (RDNA)
653 $aPATHOGEN COLONIZATION
653 $aSOYBEAN STEM CANKER
653 $aTRANSLATION ELONGATION FACTOR 1-ALPHA GENE (TEF1a)
700 1 $aSTEWART, S.
700 1 $aMONTESANO, M.
700 1 $aPONCE DE LEÓN, I.
773 $tFrontiers in Plant Science, 23 January 2020, Volume 10, Article number 1733. OPEN ACCESS. Doi: https://doi.org/10.3389/fpls.2019.01733
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