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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/06/2021 |
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Actualizado : |
25/06/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; ARTIGAS, R.; GUERRA, S.; BRANDA, A.; VÁZQUEZ, N.; NICOLINI, P.; DUTRA, F.; LLAMBÍ, S. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODY ARTIGAS, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay; SOFÍA GUERRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDREA BRANDA SICA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NOELIA VÁZQUEZ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay.; PAULA NICOLINI, Instituto Superior de la Carne, Universidad de la República (UDELAR), Tacuarembó, Uruguay.; FERNANDO DUTRA QUINTELA, División Laboratorios Veterinarios (DILAVE), Ministerio de Agricultura y Pesca (MGAP), Treinta y Tres, Uruguay.; SILVIA LLAMBÍ, Facultad de Veterinaria, Universidad de la República (UDELAR), Montevideo, Uruguay. |
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Título : |
Detection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.] |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Ciencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872 |
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ISSN : |
1678-4596 (online) |
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DOI : |
10.1590/0103-8478cr20200872 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021. |
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Contenido : |
ABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
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RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia. MenosABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed.
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RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigo... Presentar Todo |
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Palabras claves : |
Análise da curva de fusão; Bos taurus; Brachyspina syndrome; Holandês; Holstein; Melting curve analysis; PCR em tempo real; Real time PCR; Sindrome de Brachyspina. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
https://www.scielo.br/j/cr/a/kTxvzMF47Cqdb7TbPKHjxxc/?lang=en&format=pdf
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Marc : |
LEADER 03178naa a2200349 a 4500
001 1062156
005 2021-06-25
008 2021 bl uuuu u00u1 u #d
022 $a1678-4596 (online)
024 7 $a10.1590/0103-8478cr20200872$2DOI
100 1 $aFEDERICI, M.
245 $aDetection of the Brachyspina mutation in Uruguayan Holstein cows using real time PCR and melting curve analysis. [Detecção da mutação da Brachyspina em vacas Holandês Uruguaias usando PCR em tempo real e análise da curva de fusão.]$h[electronic resource]
260 $c2021
500 $aArticle history: Received 17 Sept 2020; Accepted 05 Jan 2021; Reviewed 07 Mar 2021; Publication in this collection 02 June 2021; Date of issue 2021.
520 $aABSTRACT - Brachyspina syndrome (BS) is a rare monogenic autosomal recessive hereditary disorder of the Holstein Fresian breed caused by a deletion of 3.3Kb in the Fanconi anemia complementation group I (FANCI) gene on BTA-21, which leads to a frame-shift and premature stop codon. Some of the consequences of BS are the reduction of the fertility rate and milk production. This study developed a simple, sensitive, rapid cost- effective assay method based on real time PCR and melting curve analysis for the detection of BS carrier animals. Sixty-eight normal homozygous and four heterozygous carrier genotypes were detected and confirmed through traditional PCR- electrophoresis analysis. We concluded that the assay we have developed proved to be a reliable, highly precise and low-cost tool, which could be used to monitor the presence of the BS mutation in uruguayan Holstein breed. .-.-.-.-.-.-.-.-.-.- RESUMO - A síndrome de Brachyspina (BS) é um defeito hereditário monogênico autossômico recessivo raro da raça Holstein Friesian causado por uma exclusão de 3,3 KB no gene FANCI localizado no cromossomo bovino 21, o que leva a um deslocamento de quadro e um códon de parada prematuro. Uma consequência da BS é a eficiência de reprodução reduzida e a produção de leite. O objetivo deste estudo foi o desenvolvimento de um método simples, rápido e sensível, baseado em PCR em tempo real e análise da curva de fusão para identificar animais portadores de BS. Sessenta e oito genótipos homozigotos normais e quatro heterozigotos foram detectados e confirmados através da análise tradicional de PCR e electophorese. Concluímos que o novo método é uma ferramenta confiável, altamente precisa e de baixo custo, que poderia ser usado para monitorar a presença da mutação BS na raça Holandês uruguaia.
653 $aAnálise da curva de fusão
653 $aBos taurus
653 $aBrachyspina syndrome
653 $aHolandês
653 $aHolstein
653 $aMelting curve analysis
653 $aPCR em tempo real
653 $aReal time PCR
653 $aSindrome de Brachyspina
700 1 $aARTIGAS, R.
700 1 $aGUERRA, S.
700 1 $aBRANDA, A.
700 1 $aVÁZQUEZ, N.
700 1 $aNICOLINI, P.
700 1 $aDUTRA, F.
700 1 $aLLAMBÍ, S.
773 $tCiencia Rural, 2021, volume 51 (9), Article e20200872. OPEN ACCESS. Doi: https://doi.org/10.1590/0103-8478cr20200872
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Tacuarembó. Por información adicional contacte bibliotb@tb.inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
31/01/2020 |
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Actualizado : |
10/08/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GÓMEZ, D.; SKELTON, J.; DE MARÍA, M.; HULCR, J. |
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Afiliación : |
DEMIAN FERNANDO GOMEZ DAMIANO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; School of Forest Resources and Conservation, Univ of Florida, Gainesville, USA; J. SKELTON, School of Forest Resources and Conservation, Univ of Florida, Gainesville, USA; M. DE MARÍA, Dept of Physiological Sciences, College of Veterinary Medicine, Gainesville, USA; JIRI HULCR, School of Forest Resources and Conservation, Univ of Florida, Gainesville, USA. |
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Título : |
Influence of temperature and precipitation anomaly on the seasonal emergence of invasive bark beetles in subtropical South America. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Neotropical Entomology, 1 June 2020, Volume 49, Issue 3, Pages 347-352. Doi: https://doi.org/10.1007/s13744-019-00760-y |
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DOI : |
10.1007/s13744-019-00760-y |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 18 October 2019 // Accepted 23 December 2019 // Published10 January 2020. Correspondence D.F. Gomez, School of Forest Resources and Conservation, Univ of Florida, Gainesville, USA; demiangz@gmail.com // Acknowledgments We thank Cambium Forestal Uruguay and
Weyerhaeuser Productos S.A. for helping with logistics and trapping. Funding Information: This project was funded by a cooperative agreement
with the USDA Forest Service Forest Health Protection, the project INIA FO15, and the National Science Foundation DEB Award 1556283. DG and JH were partially funded by the USDA Forest Service, JS was funded by the National Science Foundation. |
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Contenido : |
Several invasive bark beetle species have caused major economic and ecological losses in South America. Accurate predictions of beetle emergence times will make control efforts more efficient and effective. To determine whether bark beetle emergence can be predicted by season, temperature, or precipitation, we analyzed trapping records for three introduced pest species of bark beetles in Uruguay. Weused trigonometric functions as seasonal predictors in generalized linear models to account for purely seasonal effects, while testing for effects of temperature and precipitation. Results show that all three beetle species had strong but unique seasonal emergence patterns and responded differently to temperature and precipitation. Cyrtogenius luteus (Blandford) emerged in summer and increased with precipitation but was not affected by temperature. Hylurgus ligniperda (Fabricius) emerged in winter and increased with temperature but was not affected by precipitation. Orthotomicus erosus (Wollaston) had a primary emergence in spring, and a smaller emergence in early summer, but showed no significant relationship with temperature or precipitation. This study shows that the emergence of these bark beetle species in Uruguay is influenced by seasonality more than by temperature and precipitation fluctuations. It also shows how
seasonality can be easily incorporated into models to make more accurate predictions about pest population dynamics. |
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Palabras claves : |
FLIGHT ACTIVITY; FOREST PESTS; FORESTACIÓN; FORESTRY; PHENOLOGY; SCOLYTINAE; URUGUAY. |
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Asunto categoría : |
K70 Daños al bosque y protección forestal |
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Marc : |
LEADER 02955naa a2200265 a 4500
001 1060728
005 2020-08-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1007/s13744-019-00760-y$2DOI
100 1 $aGÓMEZ, D.
245 $aInfluence of temperature and precipitation anomaly on the seasonal emergence of invasive bark beetles in subtropical South America.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 18 October 2019 // Accepted 23 December 2019 // Published10 January 2020. Correspondence D.F. Gomez, School of Forest Resources and Conservation, Univ of Florida, Gainesville, USA; demiangz@gmail.com // Acknowledgments We thank Cambium Forestal Uruguay and Weyerhaeuser Productos S.A. for helping with logistics and trapping. Funding Information: This project was funded by a cooperative agreement with the USDA Forest Service Forest Health Protection, the project INIA FO15, and the National Science Foundation DEB Award 1556283. DG and JH were partially funded by the USDA Forest Service, JS was funded by the National Science Foundation.
520 $aSeveral invasive bark beetle species have caused major economic and ecological losses in South America. Accurate predictions of beetle emergence times will make control efforts more efficient and effective. To determine whether bark beetle emergence can be predicted by season, temperature, or precipitation, we analyzed trapping records for three introduced pest species of bark beetles in Uruguay. Weused trigonometric functions as seasonal predictors in generalized linear models to account for purely seasonal effects, while testing for effects of temperature and precipitation. Results show that all three beetle species had strong but unique seasonal emergence patterns and responded differently to temperature and precipitation. Cyrtogenius luteus (Blandford) emerged in summer and increased with precipitation but was not affected by temperature. Hylurgus ligniperda (Fabricius) emerged in winter and increased with temperature but was not affected by precipitation. Orthotomicus erosus (Wollaston) had a primary emergence in spring, and a smaller emergence in early summer, but showed no significant relationship with temperature or precipitation. This study shows that the emergence of these bark beetle species in Uruguay is influenced by seasonality more than by temperature and precipitation fluctuations. It also shows how seasonality can be easily incorporated into models to make more accurate predictions about pest population dynamics.
653 $aFLIGHT ACTIVITY
653 $aFOREST PESTS
653 $aFORESTACIÓN
653 $aFORESTRY
653 $aPHENOLOGY
653 $aSCOLYTINAE
653 $aURUGUAY
700 1 $aSKELTON, J.
700 1 $aDE MARÍA, M.
700 1 $aHULCR, J.
773 $tNeotropical Entomology, 1 June 2020, Volume 49, Issue 3, Pages 347-352. Doi: https://doi.org/10.1007/s13744-019-00760-y
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INIA Tacuarembó (TBO) |
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