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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/04/2018 |
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Actualizado : |
25/04/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. |
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Afiliación : |
JUAN P. GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA L. FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía; SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS |
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DOI : |
10.3389/fpls.2018.00262 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018. |
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Contenido : |
ABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress.
© 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal. MenosABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (Gm... Presentar Todo |
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Palabras claves : |
ABIOTIC STRESS; ARABIDOPSIS; DROUGHT; EIFiso4G; SOYBEAN CROP; TRANSLATION INITIATION. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9385/1/Frontiers-in-Plant-Science.-2018.fpls-09-00262.pdf
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Marc : |
LEADER 03453naa a2200289 a 4500
001 1058480
005 2018-04-25
008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2018.00262$2DOI
100 1 $aGALLINO, J.P.
245 $aA dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.
520 $aABSTRACT. Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress. © 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal.
653 $aABIOTIC STRESS
653 $aARABIDOPSIS
653 $aDROUGHT
653 $aEIFiso4G
653 $aSOYBEAN CROP
653 $aTRANSLATION INITIATION
700 1 $aRUIBAL, C.
700 1 $aCASARETTO, E.
700 1 $aFLEITAS, A.L.
700 1 $aBONNECARRERE, V.
700 1 $aBORSANI, O.
700 1 $aVIDAL, S.
773 $tFrontiers in Plant Science, 2018$gv.9, Article number 262. (2 March 2018). OPEN ACCESS
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
03/09/2019 |
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Actualizado : |
28/04/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RABAZA, A.; BANCHERO, G.; CAJARVILLE,C.; ZUNINO, P.; BRITO, A.; REPETTO, J.; FRAGA, M. |
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Afiliación : |
ANA VIRGINIA RABAZA MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CECILIA CAJARVILLE, Departamento de Nutrición Animal, Facultad de Veterinaria, Universidad de la República, San José, Uruguay; PABLO ZUNINO, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"), Montevideo, Uruguay.; Departamento de Nutriciion Animal, Instituto de Produccion Animal, Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42.5, San José 80100, Uruguay.; JOSE LUIS REPETTO CAPELLO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay.//Departamento de Bovinos, Instituto de Producción Animal, Facultad de Veterinaria, Universidad de la República, Ruta 1 km 42, CP 80100 San José, Uruguay.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Effects of feed withdrawal duration on animal behaviour, rumen microbiota and blood chemistry in feedlot cattle: implications for rumen acidosis. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Animal, Volume 14 , Issue 1 , January 2020 , pp. 66 - 77. DOI: https://doi.org/10.1017/S1751731119001538 |
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DOI : |
10.1017/S1751731119001538 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 22 October 2018 // Accepted 13 June 2019. |
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Contenido : |
Abstracts: Feed withdrawal ( FW ) is a frequent issue in open outdoor feedlot systems, where unexpected circumstances can limit the animals? access to food. The relationship among fasting period, animal behaviour during feed reintroduction ( FR ) and acidosis occurrence has not been completely elucidated. Twenty steers fitted with rumen catheters were fed a high-concentrate diet (concentrate : forage ratio 85 : 15) and were challenged by a protocol of FW followed by FR. The animals were randomly assigned to one of the four treatments: FW for 12 h ( T12 ), 24 h ( T24 ), 36 h ( T36 ) or no FW (control group) followed by FR. The steers? behaviour, ruminal chemistry, structure of the ruminal microbial community, blood enzymes and metabolites and ruminal acidosis status were assessed. Animal behaviour was affected by the FW?FR challenge (P<0.05). Steers from the T12, T24 and T36 treatments showed a higher ingestion rate and a lower frequency of rumination. Although all animals were suspected to have sub-acute ruminal acidosis ( SARA ) prior to treatment, a severe case of transient SARA arose after FR in the T12, T24 and T36 groups. The ruminal pH remained below the threshold adopted for SARA diagnosis (pH value=5.6) for more than three consecutive hours (24, 7 and 19 h in the T12, T24 and T36 treatments, respectively). The FW?FR challenge did not induce clinical acute ruminal acidosis even though steers from the T36 treatment presented ruminal pH values that were consistent with this metabolic disorder (pH threshold for acute acidosis=5.2). Total mixed ration reintroduction after the withdrawal period reactivated ruminal fermentation as reflected by changes in the fermentation end-products. Ruminal lactic acid accumulation in steers from the T24 and T36 treatments probably led to the reduction of pH in these groups. Both the FW and the FR phases may have altered the structure of the ruminal microbiota community. Whereas fibrolytic bacterial groups decreased relative abundance in the restricted animals, both lactic acid producer and utiliser bacterial groups increased (P<0.05). The results demonstrated a synchronisation between Streptococcus (lactate producer) and Megasphaera (lactate utiliser), as the relative abundance of both groups increased, suggesting that bacterial resilience may be central for preventing the onset of metabolic disturbances such as ruminal acidosis. A long-FW period (36 h) produced rumen pH reductions well below and lactic acid concentration increased well above the accepted thresholds for acute acidosis without any perceptible clinical signs. MenosAbstracts: Feed withdrawal ( FW ) is a frequent issue in open outdoor feedlot systems, where unexpected circumstances can limit the animals? access to food. The relationship among fasting period, animal behaviour during feed reintroduction ( FR ) and acidosis occurrence has not been completely elucidated. Twenty steers fitted with rumen catheters were fed a high-concentrate diet (concentrate : forage ratio 85 : 15) and were challenged by a protocol of FW followed by FR. The animals were randomly assigned to one of the four treatments: FW for 12 h ( T12 ), 24 h ( T24 ), 36 h ( T36 ) or no FW (control group) followed by FR. The steers? behaviour, ruminal chemistry, structure of the ruminal microbial community, blood enzymes and metabolites and ruminal acidosis status were assessed. Animal behaviour was affected by the FW?FR challenge (P<0.05). Steers from the T12, T24 and T36 treatments showed a higher ingestion rate and a lower frequency of rumination. Although all animals were suspected to have sub-acute ruminal acidosis ( SARA ) prior to treatment, a severe case of transient SARA arose after FR in the T12, T24 and T36 groups. The ruminal pH remained below the threshold adopted for SARA diagnosis (pH value=5.6) for more than three consecutive hours (24, 7 and 19 h in the T12, T24 and T36 treatments, respectively). The FW?FR challenge did not induce clinical acute ruminal acidosis even though steers from the T36 treatment presented ruminal pH values that were consistent with ... Presentar Todo |
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Palabras claves : |
ACIDOSIS RUMINAL; ENGORDE DE BOVINOS; FATTENING; FOOD RESTRICTION; LIVESTOCK; PLATAFORMA DE SALUD ANIMAL; RESTRICCIÓN NUTRICIONAL; RUMINAL ENVIRONMENT; SUB-ACUTE RUMINAL ACIDOSIS. |
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Thesagro : |
COMPORTAMIENTO ANIMAL; GANADO BOVINO. |
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Asunto categoría : |
L02 Alimentación animal |
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Marc : |
LEADER 03782naa a2200349 a 4500
001 1060132
005 2021-04-28
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1017/S1751731119001538$2DOI
100 1 $aRABAZA, A.
245 $aEffects of feed withdrawal duration on animal behaviour, rumen microbiota and blood chemistry in feedlot cattle$bimplications for rumen acidosis.$h[electronic resource]
260 $c2019
500 $aArticle history: Received 22 October 2018 // Accepted 13 June 2019.
520 $aAbstracts: Feed withdrawal ( FW ) is a frequent issue in open outdoor feedlot systems, where unexpected circumstances can limit the animals? access to food. The relationship among fasting period, animal behaviour during feed reintroduction ( FR ) and acidosis occurrence has not been completely elucidated. Twenty steers fitted with rumen catheters were fed a high-concentrate diet (concentrate : forage ratio 85 : 15) and were challenged by a protocol of FW followed by FR. The animals were randomly assigned to one of the four treatments: FW for 12 h ( T12 ), 24 h ( T24 ), 36 h ( T36 ) or no FW (control group) followed by FR. The steers? behaviour, ruminal chemistry, structure of the ruminal microbial community, blood enzymes and metabolites and ruminal acidosis status were assessed. Animal behaviour was affected by the FW?FR challenge (P<0.05). Steers from the T12, T24 and T36 treatments showed a higher ingestion rate and a lower frequency of rumination. Although all animals were suspected to have sub-acute ruminal acidosis ( SARA ) prior to treatment, a severe case of transient SARA arose after FR in the T12, T24 and T36 groups. The ruminal pH remained below the threshold adopted for SARA diagnosis (pH value=5.6) for more than three consecutive hours (24, 7 and 19 h in the T12, T24 and T36 treatments, respectively). The FW?FR challenge did not induce clinical acute ruminal acidosis even though steers from the T36 treatment presented ruminal pH values that were consistent with this metabolic disorder (pH threshold for acute acidosis=5.2). Total mixed ration reintroduction after the withdrawal period reactivated ruminal fermentation as reflected by changes in the fermentation end-products. Ruminal lactic acid accumulation in steers from the T24 and T36 treatments probably led to the reduction of pH in these groups. Both the FW and the FR phases may have altered the structure of the ruminal microbiota community. Whereas fibrolytic bacterial groups decreased relative abundance in the restricted animals, both lactic acid producer and utiliser bacterial groups increased (P<0.05). The results demonstrated a synchronisation between Streptococcus (lactate producer) and Megasphaera (lactate utiliser), as the relative abundance of both groups increased, suggesting that bacterial resilience may be central for preventing the onset of metabolic disturbances such as ruminal acidosis. A long-FW period (36 h) produced rumen pH reductions well below and lactic acid concentration increased well above the accepted thresholds for acute acidosis without any perceptible clinical signs.
650 $aCOMPORTAMIENTO ANIMAL
650 $aGANADO BOVINO
653 $aACIDOSIS RUMINAL
653 $aENGORDE DE BOVINOS
653 $aFATTENING
653 $aFOOD RESTRICTION
653 $aLIVESTOCK
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aRESTRICCIÓN NUTRICIONAL
653 $aRUMINAL ENVIRONMENT
653 $aSUB-ACUTE RUMINAL ACIDOSIS
700 1 $aBANCHERO, G.
700 1 $aCAJARVILLE,C.
700 1 $aZUNINO, P.
700 1 $aBRITO, A.
700 1 $aREPETTO, J.
700 1 $aFRAGA, M.
773 $tAnimal, Volume 14 , Issue 1 , January 2020 , pp. 66 - 77. DOI: https://doi.org/10.1017/S1751731119001538
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