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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
03/08/2023 |
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Actualizado : |
31/08/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MENA, E.; REBOLEDO, G.; STEWART, S.; MONTESANO, M.; PONCE DE LEÓN, I. |
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Afiliación : |
EILYN MENA, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; GUILLERMO REBOLEDO, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCOS MONTESANO, Dpto. Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Mdeo, Uruguay; Laboratorio de Fisiología Vegetal, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Mdeo, Uruguay; INÉS PONCE DE LEÓN, Departamento de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. |
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Título : |
Comparative analysis of soybean transcriptional profiles reveals defense mechanisms involved in resistance against Diaporthe caulivora. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Scientific Reports. 2023, volume 13, article 13061. https://doi.org/10.1038/s41598-023-39695-1 ----OPEN ACCESS. |
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ISSN : |
2045-2322 (online) |
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DOI : |
10.1038/s41598-023-39695-1 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2022; Accepted 29 July 2023; Published 11 August 2023 -- Correspondence author: Inés Ponce de León, iponce@iibce.edu.uy -- Supplementary material: https://www.nature.com/articles/s41598-023-39695-1#Sec20. -- FUNDING: This work was supported by "Agencia Nacional de Investigación e Innovación (ANII) (graduate fellowship and grant FCE_3_2022_1_172688)" Uruguay, "Programa de Desarrollo de las Ciencias Básicas (PEDECIBA)" Uruguay, and "Programa Grupo de I+D Comisión Sectorial de Investigación Científica, Universidad de la República", Uruguay. -- License: under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/ ) -- |
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Contenido : |
ABSTRACT.- Soybean stem canker (SSC) caused by the fungal pathogen Diaporthe caulivora is an important disease affecting soybean production worldwide. However, limited information related to the molecular mechanisms underlying soybean resistance to Diaporthe species is available. In the present work, we analyzed the defense responses to D. caulivora in the soybean genotypes Williams and Génesis 5601. The results showed that compared to Williams, Génesis 5601 is more resistant to fungal infection evidenced by significantly smaller lesion length, reduced disease severity and pathogen biomass. Transcriptional profiling was performed in untreated plants and in D. caulivora-inoculated and control-treated tissues at 8 and 48 h post inoculation (hpi). In total, 2.322 and 1.855 genes were differentially expressed in Génesis 5601 and Williams, respectively. Interestingly, Génesis
5601 exhibited a significantly higher number of upregulated genes compared to Williams at 8 hpi, 1.028 versus 434 genes. Resistance to D. caulivora was associated with defense activation through transcriptional reprogramming mediating perception of the pathogen by receptors, biosynthesis
of phenylpropanoids, hormone signaling, small heat shock proteins and pathogenesis related (PR) genes. These findings provide novel insights into soybean defense mechanisms leading to host resistance against D. caulivora, and generate a foundation for the development of resistant SSC
varieties within soybean breeding programs. © 2023 Springer Nature Limited MenosABSTRACT.- Soybean stem canker (SSC) caused by the fungal pathogen Diaporthe caulivora is an important disease affecting soybean production worldwide. However, limited information related to the molecular mechanisms underlying soybean resistance to Diaporthe species is available. In the present work, we analyzed the defense responses to D. caulivora in the soybean genotypes Williams and Génesis 5601. The results showed that compared to Williams, Génesis 5601 is more resistant to fungal infection evidenced by significantly smaller lesion length, reduced disease severity and pathogen biomass. Transcriptional profiling was performed in untreated plants and in D. caulivora-inoculated and control-treated tissues at 8 and 48 h post inoculation (hpi). In total, 2.322 and 1.855 genes were differentially expressed in Génesis 5601 and Williams, respectively. Interestingly, Génesis
5601 exhibited a significantly higher number of upregulated genes compared to Williams at 8 hpi, 1.028 versus 434 genes. Resistance to D. caulivora was associated with defense activation through transcriptional reprogramming mediating perception of the pathogen by receptors, biosynthesis
of phenylpropanoids, hormone signaling, small heat shock proteins and pathogenesis related (PR) genes. These findings provide novel insights into soybean defense mechanisms leading to host resistance against D. caulivora, and generate a foundation for the development of resistant SSC
varieties within soybean breeding program... Presentar Todo |
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Palabras claves : |
Diaporthe caulivora; Plant 32 resistance; Plant defense genes; Soybean stem canker (SSC); Transcriptomes. |
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Asunto categoría : |
-- |
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URL : |
https://www.nature.com/articles/s41598-023-39695-1.pdf
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Marc : |
LEADER 03133naa a2200265 a 4500
001 1064284
005 2023-08-31
008 2023 bl uuuu u00u1 u #d
022 $a2045-2322 (online)
024 7 $a10.1038/s41598-023-39695-1$2DOI
100 1 $aMENA, E.
245 $aComparative analysis of soybean transcriptional profiles reveals defense mechanisms involved in resistance against Diaporthe caulivora.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 30 September 2022; Accepted 29 July 2023; Published 11 August 2023 -- Correspondence author: Inés Ponce de León, iponce@iibce.edu.uy -- Supplementary material: https://www.nature.com/articles/s41598-023-39695-1#Sec20. -- FUNDING: This work was supported by "Agencia Nacional de Investigación e Innovación (ANII) (graduate fellowship and grant FCE_3_2022_1_172688)" Uruguay, "Programa de Desarrollo de las Ciencias Básicas (PEDECIBA)" Uruguay, and "Programa Grupo de I+D Comisión Sectorial de Investigación Científica, Universidad de la República", Uruguay. -- License: under a CC-BY-NC-ND 4.0 International license (http://creativecommons.org/licenses/by-nc-nd/4.0/ ) --
520 $aABSTRACT.- Soybean stem canker (SSC) caused by the fungal pathogen Diaporthe caulivora is an important disease affecting soybean production worldwide. However, limited information related to the molecular mechanisms underlying soybean resistance to Diaporthe species is available. In the present work, we analyzed the defense responses to D. caulivora in the soybean genotypes Williams and Génesis 5601. The results showed that compared to Williams, Génesis 5601 is more resistant to fungal infection evidenced by significantly smaller lesion length, reduced disease severity and pathogen biomass. Transcriptional profiling was performed in untreated plants and in D. caulivora-inoculated and control-treated tissues at 8 and 48 h post inoculation (hpi). In total, 2.322 and 1.855 genes were differentially expressed in Génesis 5601 and Williams, respectively. Interestingly, Génesis 5601 exhibited a significantly higher number of upregulated genes compared to Williams at 8 hpi, 1.028 versus 434 genes. Resistance to D. caulivora was associated with defense activation through transcriptional reprogramming mediating perception of the pathogen by receptors, biosynthesis of phenylpropanoids, hormone signaling, small heat shock proteins and pathogenesis related (PR) genes. These findings provide novel insights into soybean defense mechanisms leading to host resistance against D. caulivora, and generate a foundation for the development of resistant SSC varieties within soybean breeding programs. © 2023 Springer Nature Limited
653 $aDiaporthe caulivora
653 $aPlant 32 resistance
653 $aPlant defense genes
653 $aSoybean stem canker (SSC)
653 $aTranscriptomes
700 1 $aREBOLEDO, G.
700 1 $aSTEWART, S.
700 1 $aMONTESANO, M.
700 1 $aPONCE DE LEÓN, I.
773 $tScientific Reports. 2023, volume 13, article 13061. https://doi.org/10.1038/s41598-023-39695-1 ----OPEN ACCESS.
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| 181. |  | GIMÉNEZ, G.; LENZI, A.; IBÁÑEZ, F.; CASTILLO, A.; GONZÁLEZ-ARCOS, M.; TRUJILLO, M. INIA LBR 96.1: sabor, aroma y sanidad en un nuevo cultivar de frutilla de día neutro. Hortifruticultura. Revista INIA Uruguay, 2020, no.62, p.77-81. (Revista INIA; 62)| Tipo: Artículos en Revistas Agropecuarias |
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| 192. | | INIA LA ESTANZUELA Jornada Cultivos de Verano. La Estanzuela, Colonia, (Uruguay): INIA, set.1991. Información presentada al I Curso de Actualización Manejo y Fertilidad de Suelos, 1994 may : La Estanzuela| Biblioteca(s): INIA La Estanzuela. |
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