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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
24/01/2020 |
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Actualizado : |
10/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
MACÍAS-RIOSECO, M.; CAFFARENA, D.; FRAGA, M.; SILVEIRA, C.S.; GIANNITTI, F.; CANTÓN, G.; HECKER, Y.P.; SUANES, A.; RIET-CORREA, F. |
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Afiliación : |
MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RUBEN DARÍO CAFFARENA LEDESMA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Universidad de la República, Facultad de Veterinaria, Montevideo, Uruguay; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; University of Minnesota, Veterinary Population Medicine Department, MN, United States; GERMÁN CANTÓN, Instituto Nacional de Tecnología Agropecuaria, Balcarce, Argentina; YANINA P. HECKER, Consejo Nacional de Investigaciones Científicas y Técnicas, Balcarce, Argentina; ALEJANDRA SUANES, Ministerio de Ganadería Agricultura y Pesca, Montevideo. Dirección de Laboratorios Veterinarios, Uruguay; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Abortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Revista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008 |
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ISSN : |
2007-1124 |
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DOI : |
10.22319/rmcp.v10i4.5008 |
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Idioma : |
Inglés |
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Notas : |
Article history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018. |
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Contenido : |
ABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino. MenosABSTRACT.
In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome.
© 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved.
RESUMEN.
En noviembre de 2015 ocurrió un brote de abortos en un hato lechero... Presentar Todo |
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Palabras claves : |
Bovine abortion; Campylobacter fetus subsp. venerealis; Diagnosis of abortion; Neospora caninum; PLATAFORMA SALUD ANIMAL. |
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Asunto categoría : |
L73 Enfermedades de los animales |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14066/1/5008-14394-1-PB.pdf
http://www.ainfo.inia.uy/digital/bitstream/item/14067/1/5008-14395-1-PB.pdf
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Marc : |
LEADER 04147naa a2200313 a 4500
001 1060687
005 2020-02-10
008 2019 bl uuuu u00u1 u #d
022 $a2007-1124
024 7 $a10.22319/rmcp.v10i4.5008$2DOI
100 1 $aMACÍAS-RIOSECO, M.
245 $aAbortion outbreak caused by Campylobacter fetus subspecies venerealis and Neospora caninum in a bovine dairy herd. (Technical note). [Brote de abortos causado por Campylobacter fetus subespecie venerealis y Neospora caninum en un hato bovino lechero. (Nota de investigación)].$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 02 Agosto 2018. / Aceptado: 28 Agosto 2018.
520 $aABSTRACT. In November 2015, an abortion outbreak occurred in a commercial dairy herd of 650 Holstein cows in Florida department, Uruguay. Forty-five (45) cows aborted within 3 wk. Five fetuses were subjected to gross and microscopic pathologic examination, and microbiological testing. One fetus had fibrinous epicarditis and peritonitis, and neutrophilic bronchopneumonia. Campylobacter fetus subsp. venerealis was detected by direct immunofluorescence, isolated and identified by PCR and sequencing of the 16S rDNA in the abomasal fluid and/or lung. Histologic examination of two other fetuses revealed non-suppurative necrotizing encephalitis, lymphohistiocytic myositis and myocarditis, and lymphocytic interstitial nephritis. In these fetuses, N. caninum antigen was detected intralesionally by immunohistochemistry, and N. caninum DNA was amplified by PCR on formalin-fixed paraffin-embedded brain. Antibodies against N. caninum were detected by indirect immunofluorescence in 10 of 27 cows, with titers ranging from 1/200 to 1/3200. The results indicate that two abortigenic microorganisms may coexist and cause contemporaneous abortion in a herd. It is relevant to highlight the importance of performing multiple diagnostic tests in various aborted dams and fetuses from the same herd for the etiologic confirmation of bovine abortion syndrome. © 2019 INIFAP-CENID Parasitologia Veterinaria. All rights reserved. RESUMEN. En noviembre de 2015 ocurrió un brote de abortos en un hato lechero comercial de 650 vacas Holstein en el departamento de Florida, Uruguay. Cuarenta y cinco (45) vacas abortaron en un lapso de 3 semanas. Cinco fetos fueron sometidos a un examen patológico macro y microscópico y a pruebas microbiológicas. Un feto tenía epicarditis fibrinosa y peritonitis, así como bronconeumonía neutrofílica. Se detectó Campylobacter fetus subesp. venerealis utilizando inmunofluorescencia directa; se lo aisló e identificó mediante PCR y secuenciación del 16S rDNA en el líquido abomasal y en el pulmón. El examen histológico de otros dos fetos reveló encefalitis necrotizante no supurativa, miositis linfohistiocítica y miocarditis, y nefritis linfocítica intersticial. En estos fetos se detectó intralesionalmente el antígeno de N. caninum mediante análisis inmunohistoquímico, y se amplificó el ADN de N. caninum mediante PCR en tejido cerebral fijado con formalina y embebido en parafina. Se detectaron anticuerpos contra N. caninum mediante inmunofluorescencia indirecta en 10 de 27 vacas, con títulos de entre 1/200 y 1/3200. Los resultados indican que dos microorganismos abortígenos pueden coexistir y provocar abortos contemporáneos en un hato. Subrayamos la importancia de realizar pruebas diagnósticas múltiples en diversas madres abortadas y fetos del mismo hato para obtener una confirmación etiológica del síndrome de aborto bovino.
653 $aBovine abortion
653 $aCampylobacter fetus subsp. venerealis
653 $aDiagnosis of abortion
653 $aNeospora caninum
653 $aPLATAFORMA SALUD ANIMAL
700 1 $aCAFFARENA, D.
700 1 $aFRAGA, M.
700 1 $aSILVEIRA, C.S.
700 1 $aGIANNITTI, F.
700 1 $aCANTÓN, G.
700 1 $aHECKER, Y.P.
700 1 $aSUANES, A.
700 1 $aRIET-CORREA, F.
773 $tRevista Mexicana De Ciencias Pecuarias, 2019, Volume 10, Issue 4, 2019, Pages 1054-1063. OPEN ACCESS. Doi: 10.22319/rmcp.v10i4.5008
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
01/09/2021 |
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Actualizado : |
01/09/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
RABAZA, A.; MACÍAS-RIOSECO, M.; FRAGA, M.; UZAL, F.A.; EISLER, M.C.; RIET-CORREA, F.; GIANNITTI, F. |
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Afiliación : |
ANA VIRGINIA RABAZA MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Bristol Veterinary School, University of Bristol, Langford House, Langford, Bristol, UK.; MELISSA MACÍAS RIOSECO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; California Animal Health and Food Safety (CAHFS) Laboratory, University of California At Davis, Davis, CA, USA.; MARTIN FRAGA COTELO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FRANCISCO A UZAL, California Animal Health and Food Safety (CAHFS) Laboratory, University of California At Davis, Davis, CA, USA.; MARK C EISLER, Bristol Veterinary School, University of Bristol, Langford House, Langford, Bristol, UK.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Programa de Pós Graduação Em Ciência Animal Nos Trópicos, Faculdade de Veterinária, Universidade Federal da Bahia, Ondina, Salvador, BA, Brazil.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Coxiella burnetii abortion in a dairy farm selling artisanal cheese directly to consumers and review of Q fever as a bovine abortifacient in South America and a human milk-borne disease. (Veterinary Microbiology :(Review). |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Brazilian Journal of Microbiology, 2021. Doi: https://doi.org/10.1007/s42770-021-00593-1 |
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DOI : |
10.1007/s42770-021-00593-1 |
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Idioma : |
Inglés |
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Contenido : |
Abstract: Coxiella burnetii is a highly transmissible intracellular bacterium with a low infective dose that causes Q fever (coxiellosis), a notifiable zoonotic disease distributed worldwide. Livestock are the main source of C. burnetii transmission to humans, which occurs mostly through the aerogenous route. Although C. burnetii is a major abortifacient in small ruminants, it is less frequently diagnosed in aborting cattle. We report a case of C. burnetii abortion in a lactating Holstein cow from a dairy farm producing and selling artisanal cheese directly to consumers in Uruguay, and review the literature on coxiellosis as a bovine abortifacient in South America and as a milk-borne disease. The aborted cow had severe necrotizing placentitis with abundant intratrophoblastic and intralesional C. burnetii confirmed by immunohistochemistry and PCR. After primo-infection in cattle, C. burnetii remains latent in the lymph nodes and mammary glands, with milk being a significant and persistent excretion route. Viable C. burnetii has been found in unpasteurized milk and cheeses after several months of maturing. The risk of coxiellosis after the consumption of unpasteurized dairy products, including cheese, is not negligible. This report raises awareness on bovine coxiellosis as a potential food safety problem in on-farm raw cheese manufacturing and sales. The scant publications on abortive coxiellosis in cattle in South America suggest that the condition has probably gone underreported in all countries of this subcontinent except for Uruguay. Therefore, we also discuss the diagnostic criteria for laboratory-based confirmation of C. burnetii abortion in ruminants as a guideline for veterinary diagnosticians. MenosAbstract: Coxiella burnetii is a highly transmissible intracellular bacterium with a low infective dose that causes Q fever (coxiellosis), a notifiable zoonotic disease distributed worldwide. Livestock are the main source of C. burnetii transmission to humans, which occurs mostly through the aerogenous route. Although C. burnetii is a major abortifacient in small ruminants, it is less frequently diagnosed in aborting cattle. We report a case of C. burnetii abortion in a lactating Holstein cow from a dairy farm producing and selling artisanal cheese directly to consumers in Uruguay, and review the literature on coxiellosis as a bovine abortifacient in South America and as a milk-borne disease. The aborted cow had severe necrotizing placentitis with abundant intratrophoblastic and intralesional C. burnetii confirmed by immunohistochemistry and PCR. After primo-infection in cattle, C. burnetii remains latent in the lymph nodes and mammary glands, with milk being a significant and persistent excretion route. Viable C. burnetii has been found in unpasteurized milk and cheeses after several months of maturing. The risk of coxiellosis after the consumption of unpasteurized dairy products, including cheese, is not negligible. This report raises awareness on bovine coxiellosis as a potential food safety problem in on-farm raw cheese manufacturing and sales. The scant publications on abortive coxiellosis in cattle in South America suggest that the condition has probably gone underrepo... Presentar Todo |
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Palabras claves : |
Abortion; Dairy production; Food safety; Milk-borne disease; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Q fever; Zoonosis. |
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Asunto categoría : |
L01 Ganadería |
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Marc : |
LEADER 02785naa a2200301 a 4500
001 1062381
005 2021-09-01
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1007/s42770-021-00593-1$2DOI
100 1 $aRABAZA, A.
245 $aCoxiella burnetii abortion in a dairy farm selling artisanal cheese directly to consumers and review of Q fever as a bovine abortifacient in South America and a human milk-borne disease. (Veterinary Microbiology$b(Review).$h[electronic resource]
260 $c2021
520 $aAbstract: Coxiella burnetii is a highly transmissible intracellular bacterium with a low infective dose that causes Q fever (coxiellosis), a notifiable zoonotic disease distributed worldwide. Livestock are the main source of C. burnetii transmission to humans, which occurs mostly through the aerogenous route. Although C. burnetii is a major abortifacient in small ruminants, it is less frequently diagnosed in aborting cattle. We report a case of C. burnetii abortion in a lactating Holstein cow from a dairy farm producing and selling artisanal cheese directly to consumers in Uruguay, and review the literature on coxiellosis as a bovine abortifacient in South America and as a milk-borne disease. The aborted cow had severe necrotizing placentitis with abundant intratrophoblastic and intralesional C. burnetii confirmed by immunohistochemistry and PCR. After primo-infection in cattle, C. burnetii remains latent in the lymph nodes and mammary glands, with milk being a significant and persistent excretion route. Viable C. burnetii has been found in unpasteurized milk and cheeses after several months of maturing. The risk of coxiellosis after the consumption of unpasteurized dairy products, including cheese, is not negligible. This report raises awareness on bovine coxiellosis as a potential food safety problem in on-farm raw cheese manufacturing and sales. The scant publications on abortive coxiellosis in cattle in South America suggest that the condition has probably gone underreported in all countries of this subcontinent except for Uruguay. Therefore, we also discuss the diagnostic criteria for laboratory-based confirmation of C. burnetii abortion in ruminants as a guideline for veterinary diagnosticians.
653 $aAbortion
653 $aDairy production
653 $aFood safety
653 $aMilk-borne disease
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aQ fever
653 $aZoonosis
700 1 $aMACÍAS-RIOSECO, M.
700 1 $aFRAGA, M.
700 1 $aUZAL, F.A.
700 1 $aEISLER, M.C.
700 1 $aRIET-CORREA, F.
700 1 $aGIANNITTI, F.
773 $tBrazilian Journal of Microbiology, 2021. Doi: https://doi.org/10.1007/s42770-021-00593-1
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