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| 3. |  | BARLOCCO, C.; MAYANS, M.; MORTALENA, M.; LOPEZ, V.; BEYHAUT, E. Sistema uruguayo de fiscalización de inoculantes: complementación interinstitucional. P16. [abstract + poster]. Módulo 5: Desarrollos y perspectivas de empresas productoras y usuarias de bioinsumos. In: Abreo, E.; Beyhaut, E.; Rivas, F. (Eds.). Simposio Microorganismos para la Agricultura, 2. [Resúmenes y Posters]. Canelones (UY): INIA, 2022. p.43. (Serie Actividades de Difusión; 801).| Biblioteca(s): INIA Las Brujas. |
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| 8. | | RUIZ GONZÁLEZ, S.; ROSAS, J.E.; MATTOS, N.; MORTALENA, M.; LOPEZ, V.; BARLOCCO, C.; BEYHAUT, E. Mapeo asociativo de la fijación biológica de nitrógeno en germoplasma avanzado del programa de mejoramiento genético de soja de INIA. O6. Módulo 2: Integración de información sobre el microbioma en el manejo de sistemas de producción y en el mejoramiento genético. In: Abreo, E.; Beyhaut, E.; Rivas, F. (Eds.). Simposio Microorganismos para la Agricultura, 2. [Resúmenes y Posters]. Canelones (UY): INIA, 2022. p.14. (Serie Actividades de Difusión; 797)801 Proyecto: Maximización de la fijación biológica de nitrógeno en el cultivo de soja. Beca: INIA. Fuente financiamiento: INIA.| Biblioteca(s): INIA Las Brujas. |
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| 9. | | ABREO, E.; BEYHAUT, E.; RIVAS, F.; BARLOCCO, C.; GARAYCOCHEA, S.; LATTANZI, F.; REYNO, R. Microorganismos como bioinsumos para la agricultura: ecología, selección, formulación y uso. Revista INIA Uruguay, Setiembre 2021, no.66, p. 119-121. (Revista INIA; 66).| Biblioteca(s): INIA Treinta y Tres. |
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| 11. | | ALTIER, N.; ABREO, E.; BEYHAUT, E.; RIVAS, F.; BARLOCCO, C.; GARAYCOCHEA, S.; DINI, B.; LÓPEZ, V.; MATTOS, N.; MORTALENA, M. Plataforma de Bioinsumos de uso agrícola: desarrollo de tecnologías y productos basados en microorganismos benéficos. Revista INIA Uruguay, 2020, no. 61, p. 85-90. (Revista INIA; 61).| Biblioteca(s): INIA Las Brujas. |
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| 12. | | DI SALVO, L.; GARCÍA, J.E.; PUENTE, M.L.; AMIGO, J.; ANRIQUEZ, A.; BARLOCCO, C.; BENINTENDE, S.; BOCHATAY, T.; BORTOLATO, M.; CASSÁN, F.; CASTAÑO, C.; CATAFESTA, M.; CONIGLIO, A.; DÍAZ, M.; GALIÁN, L.R.; GALLACE, E.; GARCÍA, P.; GARCÍA DE SALAMONE, I.E.; LANDA, M.; LIERNUR, G.; MANEIRO, M.L.; MASSA, R.; MALINVERNI, J.; MARCHESSI, N.; MONTELEONE, E.; OVIEDO, S.; POBLITI, L.; PORTELA, G.; RADOVANCICH, D; RIGHES, S.; ROCHA, R.; RODRÍGUEZ CÁCERES, E.; ROSSI, A.; SANTELLA, G.; TORTORA, M.L.; TREJO, N.; VALENZUELA, J.A.; VALLEJO, D. The drop plate method as an alternative for Azospirillum spp viable cell enumeration within the consensus protocol of the REDCAI network. (Brief report). [La técnica de la microgota como alternativa para el recuento de Azospirillum spp. dentro del protocolo de la Red de Control de Calidad de Inoculantes (REDCAI).] Revista Argentina de Microbiología, 2022, Volume 54, Issue 2, Pages 152-157. OPEN ACCESS. doi: https://doi.org/10.1016/j.ram.2021.05.002 Article history: Received 24 November 2020, Accepted 12 May 2021, Available online 7 July 2021. -- Corresponding author. M.L. Puente, e-mail address: puente.mariana@inta.gob.ar -- LICENSE: Este es un artículo Open Access bajo la licencia...| Biblioteca(s): INIA Las Brujas. |
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Registro completo
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Biblioteca (s) : |
INIA Tacuarembó. |
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Fecha actual : |
30/05/2019 |
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Actualizado : |
04/05/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
WALLACE, F.; BENNADJI, Z.; FERREIRA, F.; OLIVARO, C. |
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Afiliación : |
FEDERICO WALLACE, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay.; ZOHRA BENNADJI SOUALHIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO AMARY FERREIRA CHIESA, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay; Laboratorio de Carbohidratos y Glicoconjugados, Departamento de Química Orgánica, Facultad de Química, Udelar, Instituto de Higiene, Uruguay.; CRISTINA OLIVARO, Espacio de Ciencia y Tecnología Química, Centro Universitario de Tacuarembó, UdelaR, Tacuarembó, Uruguay. |
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Título : |
Structural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry.
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
Phytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837 |
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DOI : |
10.1002/pca.2837 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00);
the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química. |
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Contenido : |
Abstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work. MenosAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as
immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of
the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methyla... Presentar Todo |
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Palabras claves : |
IMMUNOADJUVANT SAPONINS; MASS SPECTROMETRY; METHYLATION ANALYSIS; MONOSACCHARIDE ANALYIS; QUILLAJA BRASILIENSIS. |
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Asunto categoría : |
K11 Ingeniería forestal |
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Marc : |
LEADER 02910naa a2200241 a 4500
001 1059792
005 2020-05-04
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1002/pca.2837$2DOI
100 1 $aWALLACE, F.
245 $aStructural characterisation of new immunoadjuvant saponins from leaves and the first study of saponins from the bark of Quillaja brasiliensis by liquid chromatography electrospray ionisation ion trap mass spectrometry. .$h[electronic resource]
260 $c2019
500 $aArticle history: Received: 19 December 2018; Revised: 21 February 2019; Accepted: 27 March 2019. ACKNOWLEDGEMENTS: This work was supported by the INIA (award L4?FO?21?0?00); the MEC (Uruguay, Fondo Vaz Ferreira fvf_2017_158) and the PEDECIBA-Química.
520 $aAbstract. Introduction: Quillaja brasiliensis (St. A. ?Hil. & Tul) Mart (Quillajaceae) is a species native to South America, which is rich in saponins. Saponins are used in different industries, so there is a constant demand for this type of compound. Based on the wide range of applications for the saponins found in this species, notably as immunoadjuvants, we conducted a comprehensive study of this tree and its saponins. Objective: The purpose of this work is to complete the characterisation of the immunoadjuvant saponin fraction from Q. brasiliensis leaves and further study the saponin fraction obtained from Q. brasiliensis bark. Methodology: Saponin fractions were studied using mass spectrometry in combination with classical methods of monosaccharide and methylation analysis. We performed direct infusion and liquid chromatography/electrospray ionisation ion trap multiple?stage mass spectrometry (DI?ESI?IT?MSn and LC?ESI?IT?MS2). Results: Seventy?five saponins, 21 from leaves and 54 from bark, were tentatively identified according to their molecular mass, fragmentation pattern and chromatographic behaviour. This work represents the first investigation of saponins from the bark of Q. brasiliensis and some of them presented new structural motifs not previously reported in the genus Quillaja. Conclusion: The efficiency and selectivity of the data dependent LC?MS2 method allowed the rapid profiling of saponins from Q. brasiliensis. The results of the monosaccharide and methylation analysis performed in saponins from Q. brasiliensis fractions and Q. saponaria Molina (Quillajaceae) fraction gives further support to the structures proposed according to the mass spectral data, validating the strategy used in the present work.
653 $aIMMUNOADJUVANT SAPONINS
653 $aMASS SPECTROMETRY
653 $aMETHYLATION ANALYSIS
653 $aMONOSACCHARIDE ANALYIS
653 $aQUILLAJA BRASILIENSIS
700 1 $aBENNADJI, Z.
700 1 $aFERREIRA, F.
700 1 $aOLIVARO, C.
773 $tPhytochemical Analysis, 1 November 2019, Volume 30, Issue 6, Pages 644-652. Doi: https://doi.org/10.1002/pca.2837
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