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 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
15/08/2022 |
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Actualizado : |
01/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
PASSOS, J.R.S.; GUERREIRO, D.D.; KAMILA S. OTÁVIO; DOS SANTOS-NETO, P.C.; SOUZA-NEVES, M.; CUADRO, F.; NUÑEZ?OLIVERA, R.; CRISPO, M.; VASCONCELOS, F.R.; BEZERRA, M.J.B.; SILVA, R.F.; LIMA, L.F.; FIGUEIREDO, J.R.; BUSTAMANTE-FILHO, I.C.; MENCHACA, A.; MOURA, A.A. |
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Afiliación : |
JOSÉ RENATO S. PASSOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; DENISE D. GUERREIRO, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; OTÁVIO, K.S., Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; PEDRO C. DOS SANTOS-NETO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARCELA SOUZA-NEVES, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; FEDERICO CUADRO, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; RICHARD NUÑEZ?OLIVERA, Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; MARTINA CRIPO, Unidad de Biotecnología en Animales de Laboratorio, Institut Pasteur de Montevideo, Montevideo, Uruguay.; FÁBIO R. VASCONCELOS, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; MARIA JULIA B. BEZERRA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil.; RENATO F. SILVA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; LARITZA F. LIMA, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; JOSÉ RICARDO FIGUEIREDO, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), Ceará State University, Fortaleza, Brazil.; IVAN C. BUSTAMANTE-FILHO, Laboratório de Biotecnologia, Universidade do Vale do Taquari, Lajeado, Brazil.; JOSE ALEJO MENCHACA BARBEITO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Instituto de Reproducción Animal Uruguay, Fundación IRAUy, Montevideo, Uruguay.; ARLINDO A. MOURA, Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil. |
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Título : |
How in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?. |
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Complemento del título : |
Volume 89, Issue 10, Pages 459 - 470October 2022 |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Molecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638 |
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DOI : |
10.1002/mrd.23638 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil. |
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Contenido : |
Abstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC. MenosAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols ... Presentar Todo |
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Palabras claves : |
FOLLICLE; OVARY; OVINE; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; PROTEINS; REPRODUCTION. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 03884naa a2200409 a 4500
001 1063525
005 2022-12-01
008 2022 bl uuuu u00u1 u #d
024 7 $a10.1002/mrd.23638$2DOI
100 1 $aPASSOS, J.R.S.
245 $aHow in vitro maturation changes the proteome of ovine cumulus-oocyte complexes?.$h[electronic resource]
260 $c2022
500 $aArticle history: Received: 16 February 2022 | Accepted: 21 July 2022. -- Corresponding author: Moura, A.A.; Laboratory of Animal Physiology, Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; email:arlindo.moura@gmail.com -- Funding: The experiments presently described were conducted at the facilities of the Instituto de Reproducción Animal Uruguay (Fundacion IRAUy, Montevideo, Uruguay) and at the Unidad de Biotecnología en Animales de Laboratorio (UBAL) of the Institut Pasteur de Montevideo, Uruguay. Specially, the authors thank Dr. Rosario Durán and Dr. Alejandro Leyva for kindly assisting us in the proteomic experiment. Financial support was provided by Fundacion IRAUy; PRONEX 02/2015 (Programa de Apoio a Núcleos de Excelência Pronex/Funcap/CNPq); The Brazilian Research Council-CNPq (grants # 313160/2017-1 and 438773/2018-7); Brazilian Commission for Higher Education (CAPES); Ceará State Foundation for the Support of Technology and Scientific Development (FUNCAP), Brazil.
520 $aAbstract: The present study evaluated the effects of in vitro maturation (IVM) on the proteome of cumulus-oocyte complexes (COCs) from ewes. Extracted COC proteins were analyzed by LC-MS/MS. Differences in protein abundances (p < 0.05) and functional enrichments in immature versus in vitro-matured COCs were evaluated using bioinformatics tools. There were 2550 proteins identified in the COCs, with 89 and 87 proteins exclusive to immature and mature COCs, respectively. IVM caused downregulation of 84 and upregulation of 34 proteins. Major upregulated proteins in mature COCs were dopey_N domain-containing protein, structural maintenance of chromosomes protein, ubiquitin-like modifier-activating enzyme 2. Main downregulated proteins in mature COCs were immunoglobulin heavy constant mu, inter-alpha-trypsin inhibitor heavy chain 2, alpha-2-macroglobulin. Proteins exclusive to mature COCs and upregulated after IVM related to immune response, complement cascade, vesicle-mediated transport, cell cycle, and extracellular matrix organization. Proteins of immature COCs and downregulated after IVM were linked to metabolic processes, immune response, and complement cascade. KEGG pathways and miRNA-regulated genes attributed to downregulated and mature COC proteins related to complement and coagulation cascades, metabolism, humoral response, and B cell-mediated immunity. Thus, IVM influenced the ovine COC proteome. This knowledge supports the future development of efficient IVM protocols for Ovis aries. © 2022 Wiley Periodicals LLC.
653 $aFOLLICLE
653 $aOVARY
653 $aOVINE
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aPROTEINS
653 $aREPRODUCTION
700 1 $aGUERREIRO, D.D.
700 1 $aKAMILA S. OTÁVIO
700 1 $aDOS SANTOS-NETO, P.C.
700 1 $aSOUZA-NEVES, M.
700 1 $aCUADRO, F.
700 1 $aNUÑEZ?OLIVERA, R.
700 1 $aCRISPO, M.
700 1 $aVASCONCELOS, F.R.
700 1 $aBEZERRA, M.J.B.
700 1 $aSILVA, R.F.
700 1 $aLIMA, L.F.
700 1 $aFIGUEIREDO, J.R.
700 1 $aBUSTAMANTE-FILHO, I.C.
700 1 $aMENCHACA, A.
700 1 $aMOURA, A.A.
773 $tMolecular reproduction and development, October 2022, Volume 89, Issue 10, pages 459-470. doi: https://doi.org/10.1002/mrd.23638
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
22/08/2018 |
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Actualizado : |
06/12/2018 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
ACUÑA, H.; INOSTROZA, L.; PINOCHET, D.; BALOCCHI, O.; ORTEGA, F.; ROMERO, O.; LÓPEZ, I.; SEGUEL, I.; TAPIA, G.; TORRES, A.; TEUBER, N.; GALDAMES, R.; REBUFFO, M. |
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Afiliación : |
H. ACUÑA, Facultad de Agronomía, Universidad de Concepción, Chile; L. INOSTROZA, CRI Quilamapu, Instituto de Investigaciones Agropecuarias INIA, Chile; D. PINOCHET, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; O. BALOCCHI, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; F. ORTEGA, CRI Carillanca, INIA, Chile; O. ROMERO, CRI Carillanca, INIA, Chile; I. LÓPEZ, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Chile; I. SEGUEL, CRI Carillanca, INIA, Chile; G. TAPIA, CRI Quilamapu, Instituto de Investigaciones Agropecuarias INIA, Chile; A. TORRES, CRI Remehue, INIA, Chile; N. TEUBER, Departamento Técnico, COVEPA SpA, Chile; R. GALDAMES, CRI Carillanca, INIA, Chile; MONICA IRENE REBUFFO GFELLER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
White clover (Trifolium repens L.) germplasm selection for abiotic stress tolerance from naturalized populations collected in the southern regions of Chile . |
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Fecha de publicación : |
2016 |
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Fuente / Imprenta : |
In: Roldán-Ruiz I., Baert J., Reheul D. (eds). Breeding in a World of Scarcity. Springer, Cham (Chap.12). |
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Páginas : |
pp 81-87. |
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ISBN : |
978-3-319-28930-4 // Online ISBN 978-3-319-28932-8 |
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DOI : |
10.1007/978-3-319-28932-8_12 |
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Idioma : |
Inglés |
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Notas : |
Chapter history: First Online 14 June 2016. |
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Contenido : |
ABSTRACT.
Nine white clover populations (WCP) naturalized in Chile were evaluated under controlled (greenhouse pot experiments) and field conditions for water and P stress. The objective of this work was to determine dry matter production and water use and P efficiencies to identify which naturalized white clover populations would be most useful for breeding programs. In the
field experiments the clover was grown mixed with perennial ryegrass, but in pots the clover was grown alone. The treatments were the factorial combinations of WCP by water and P levels; completely randomized and complete randomized block experimental designs were used for pot and field experiments, respectively. The population WCP 9-1-X was the most
efficient for using water under controlled conditions and was among the three with the highest density of stolons under non-irrigated field conditions. The naturalized WCP 8-1-X produced more clover DM at the low P level than at the high P level and reached the same DM yield as cultivar Huia, which surpassed all the naturalized populations. These genetic
materials have high potential to be included in white clover breeding programs whose objective is to generate cultivars for water- restricted and/or P-defi cient marginal soils.
© Springer International Publishing Switzerland 2016 |
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Palabras claves : |
P EFFICIENCY; POT AND FIELD EXPERIMENTS; SOIL P STRESS; WATER STRESS; WATER USE EFFICIENCY. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02465naa a2200361 a 4500
001 1058932
005 2018-12-06
008 2016 bl uuuu u00u1 u #d
024 7 $a10.1007/978-3-319-28932-8_12$2DOI
100 1 $aACUÑA, H.
245 $aWhite clover (Trifolium repens L.) germplasm selection for abiotic stress tolerance from naturalized populations collected in the southern regions of Chile .$h[electronic resource]
260 $c2016
300 $app 81-87.
500 $aChapter history: First Online 14 June 2016.
520 $aABSTRACT. Nine white clover populations (WCP) naturalized in Chile were evaluated under controlled (greenhouse pot experiments) and field conditions for water and P stress. The objective of this work was to determine dry matter production and water use and P efficiencies to identify which naturalized white clover populations would be most useful for breeding programs. In the field experiments the clover was grown mixed with perennial ryegrass, but in pots the clover was grown alone. The treatments were the factorial combinations of WCP by water and P levels; completely randomized and complete randomized block experimental designs were used for pot and field experiments, respectively. The population WCP 9-1-X was the most efficient for using water under controlled conditions and was among the three with the highest density of stolons under non-irrigated field conditions. The naturalized WCP 8-1-X produced more clover DM at the low P level than at the high P level and reached the same DM yield as cultivar Huia, which surpassed all the naturalized populations. These genetic materials have high potential to be included in white clover breeding programs whose objective is to generate cultivars for water- restricted and/or P-defi cient marginal soils. © Springer International Publishing Switzerland 2016
653 $aP EFFICIENCY
653 $aPOT AND FIELD EXPERIMENTS
653 $aSOIL P STRESS
653 $aWATER STRESS
653 $aWATER USE EFFICIENCY
700 1 $aINOSTROZA, L.
700 1 $aPINOCHET, D.
700 1 $aBALOCCHI, O.
700 1 $aORTEGA, F.
700 1 $aROMERO, O.
700 1 $aLÓPEZ, I.
700 1 $aSEGUEL, I.
700 1 $aTAPIA, G.
700 1 $aTORRES, A.
700 1 $aTEUBER, N.
700 1 $aGALDAMES, R.
700 1 $aREBUFFO, M.
773 $tIn: Roldán-Ruiz I., Baert J., Reheul D. (eds). Breeding in a World of Scarcity. Springer, Cham (Chap.12).
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