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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
06/10/2022 |
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Actualizado : |
06/10/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GIANNITTI, F.; SILVEIRA, C.S.; BULLOCK, H.; BERON, M.; FERNÁNDEZ-CIGANDA, S.; BENÍTEZ-GALEANO, M.J.; RODRÍGUEZ-OSORIO, N.; SILVA-FLANNERY, L.; PERDOMO, T.; CABRERA, A.; PUENTES, R.; COLINA, R.; RITTER, J.M.; CASTELLS, M. |
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Afiliación : |
FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINE DA SILVA SILVEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; HANNAH BULLOCK, Synergy America Inc., Atlanta, GA 30329, USA; MARINA MAURENTE BERON, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SOFÍA FERNÁNDEZ-CIGANDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA JOSÉ BENÍTEZ-GALEANO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; NÉLIDA RODRÍGUEZ-OSORIO, Unidad de Genómica y Bioinformática, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; LUCIANA SILVA-FLANNERY, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; TERESITA YISELL PERDOMO TORRES, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS CABRERA, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; Laboratorio de Interacciones Hospedero-Patógeno, Institut Pasteur de Montevideo, Montevideo 11400, Uruguay; RODRIGO PUENTES, Facultad de Veterinaria, Universidad de la República, Montevideo 13000, Uruguay; RODNEY COLINA, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay; JANA M. RITTER, Infectious Diseases Pathology Branch, Centers for Disease Control and Prevention (CDC), Atlanta, GA 30329, USA; MATÍAS CASTELLS, Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto 50000, Uruguay. |
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Título : |
Bovine Polyomavirus-1 (Epsilonpolyomavirus bovis): An emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Viruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042 |
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ISSN : |
1999-4915 |
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DOI : |
10.3390/v14092042 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022.
Academic Editors: Fernando Bauermann and Mayara Maggioli.
Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com --
Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock |
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Contenido : |
ABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors. MenosABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus mor... Presentar Todo |
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Palabras claves : |
Abortion; Cattle; Emerging diseases; Epsilonpolyomavirus bovis; Nephropathy; Next generation sequencing; Pathology; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; Polyomavirus; Reproductive diseases; Viral diseases. |
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Asunto categoría : |
L20 Ecología animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16810/1/viruses-14-02042-v2.pdf
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Marc : |
LEADER 04533naa a2200445 a 4500
001 1063641
005 2022-10-06
008 2022 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $a10.3390/v14092042$2DOI
100 1 $aGIANNITTI, F.
245 $aBovine Polyomavirus-1 (Epsilonpolyomavirus bovis)$bAn emerging fetal pathogen of cattle that causes renal lesions resembling Polyomavirus-associated nephropathy of humans.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 12 August 2022; Revised 8 September 2022: Accepted 9 September 2022; Published 14 September 2022. Academic Editors: Fernando Bauermann and Mayara Maggioli. Correspondence authors: Giannitti, F.; Plataforma de Investigación en Salud Animal, Instituto Nacional de Investigación Agropecuaria (INIA), Estación Experimental La Estanzuela, Colonia, Uruguay; email:fgiannitti@inia.org.uy - Castells, M.; Laboratorio de Virología Molecular, Departamento de Ciencias Biológicas, Centro Universitario Regional (CENUR) Litoral Norte, Universidad de la República, Salto, Uruguay; email:matiascastellsbauer@gmail.com -- Funding: Instituto Nacional de Investigación Agropecuaria (INIA), grant PL_27 N-23398. -- This article belongs to the Special Issue Pathogenesis and Host Responses to Viral Diseases in Livestock Species: https://www.mdpi.com/journal/viruses/special_issues/pathogenesis_livestock
520 $aABSTRACT.- Bovine polyomavirus-1 (BoPyV-1, Epsilonpolyomavirus bovis) is widespread in cattle and has been detected in commercialized beef at supermarkets in the USA and Germany. BoPyV-1 has been questioned as a probable zoonotic agent with documented increase in seropositivity in people exposed to cattle. However, to date, BoPyV-1 has not been causally associated with pathology or disease in any animal species, including humans. Here we describe and illustrate pathological findings in an aborted bovine fetus naturally infected with BoPyV-1, providing evidence of its pathogenicity and probable abortigenic potential. Our results indicate that: (i) BoPyV-1 can cause severe kidney lesions in cattle, including tubulointerstitial nephritis with cytopathic changes and necrosis in tubular epithelial cells, tubular and interstitial inflammation, and interstitial fibroplasia; (ii) lesions are at least partly attributable to active viral replication in renal tubular epithelial cells, which have abundant intranuclear viral inclusions; (iii) BoPyV-1 large T (LT) antigen, resulting from early viral gene expression, can be detected in infected renal tubular epithelial cells using a monoclonal antibody raised against Simian Virus-40 polyomavirus LT antigen; and (iv) there is productive BoPyV-1 replication and virion assembly in the nuclei of renal tubular epithelial cells, as demonstrated by the ultrastructural observation of abundant arrays of viral particles with typical polyomavirus morphology. Altogether, these lesions resemble the "cytopathic-inflammatory pathology pattern" proposed in the pathogenesis of Human polyomavirus-1-associated nephropathy in immunocompromised people and kidney allograft recipients. Additionally, we sequenced the complete genome of the BoPyV-1 infecting the fetus, which represents the first whole genome of a BoPyV-1 from the Southern Hemisphere. Lastly, the BoPyV-1 strain infecting this fetus was isolated, causing a cytopathic effect in Madin-Darby bovine kidney cells. We conclude that BoPyV-1 is pathogenic to the bovine fetus under natural circumstances. Further insights into the epidemiology, biology, clinical relevance, and zoonotic potential of BoPyV-1 are needed. © 2022 by the authors.
653 $aAbortion
653 $aCattle
653 $aEmerging diseases
653 $aEpsilonpolyomavirus bovis
653 $aNephropathy
653 $aNext generation sequencing
653 $aPathology
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPolyomavirus
653 $aReproductive diseases
653 $aViral diseases
700 1 $aSILVEIRA, C.S.
700 1 $aBULLOCK, H.
700 1 $aBERON, M.
700 1 $aFERNÁNDEZ-CIGANDA, S.
700 1 $aBENÍTEZ-GALEANO, M.J.
700 1 $aRODRÍGUEZ-OSORIO, N.
700 1 $aSILVA-FLANNERY, L.
700 1 $aPERDOMO, T.
700 1 $aCABRERA, A.
700 1 $aPUENTES, R.
700 1 $aCOLINA, R.
700 1 $aRITTER, J.M.
700 1 $aCASTELLS, M.
773 $tViruses, 2022; 14 (9): 2042. OPEN ACCESS. doi: https://doi.org/10.3390/v14092042
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INIA Las Brujas (LB) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
02/10/2017 |
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Actualizado : |
02/10/2017 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CONIBERTI, A.; FERRARI, V.; DISEGNA, E.; GARCÍA PETILLO, M.; LAKSO, A.N. |
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Afiliación : |
ANDRES CONIBERTI MUNDY, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; VIRGINIA PAULINA FERRARI MORENA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; EDGARDO JOSE DISEGNA LIGUORI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIO GARCÍA PETILLO, Departamento de Suelos y Aguas, Facultad de Agronomía, UdelaR.; A.N. LAKSO, Department of Horticulture, College of Agriculture and Life Science, Cornell University. |
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Título : |
Under-trellis cover crop and planting density to achieve vine balance in a humid climate. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Scientia Horticulturae, 2018, v. 227, p. 65?74. |
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ISSN : |
0304-4238 |
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DOI : |
10.1016/j.scienta.2017.09.012 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 25 June 2017; Received in revised form 24 August 2017; Accepted 16 September 2017; Available online 28 September 2017. |
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Contenido : |
ABSTRACT.
The goal of our study was to improve Tannat Vitis vinifera L. grape and wine composition, by achieving vine balance in a humid climate. We tested under-trellis cover crops (UTCC) compared to a standard floor management of alleyway cover crops and under-vine herbicide. This strategy was tested in combination with variable planting density over three growing seasons in Southern Uruguay. Two factors were evaluated in a split plot design with five replicates. Treatments were, (1) UTCC (full cover of the vineyard soil with tall fescue (Festuca
arundinacea Shreb)) versus conventional alleyway tall fescue with 1.0 m wide weed-free strips under the trellis, and (2) two spacings between vines in the row (0.8 vs 1.5 m). To avoid excessive vine water stress, supplemental irrigation was used during water deficit periods. Shoot growth rate, mid-day stem water potential, berry size and berry composition were monitored over the season as well as final yield, cluster and pruning weights. Results showed that UTCC reduced vegetative growth as expressed by pruning weight/m while closer PD resulted in greater vegetative growth parameters. UTCC reduced vine vegetative growth to recommended values of pruning weight per m of row under both plating densities. It also reduced berry size, cluster weight and bunch rot incidence as well as increased total soluble solids and anthocyanin concentration in grapes compared to the standard herbicide treatment. The use of UTCC with supplemental irrigation, showed promise for achieving vine balance in high vine capacity conditions.
© 2017 Elsevier B.V. All rights reserved MenosABSTRACT.
The goal of our study was to improve Tannat Vitis vinifera L. grape and wine composition, by achieving vine balance in a humid climate. We tested under-trellis cover crops (UTCC) compared to a standard floor management of alleyway cover crops and under-vine herbicide. This strategy was tested in combination with variable planting density over three growing seasons in Southern Uruguay. Two factors were evaluated in a split plot design with five replicates. Treatments were, (1) UTCC (full cover of the vineyard soil with tall fescue (Festuca
arundinacea Shreb)) versus conventional alleyway tall fescue with 1.0 m wide weed-free strips under the trellis, and (2) two spacings between vines in the row (0.8 vs 1.5 m). To avoid excessive vine water stress, supplemental irrigation was used during water deficit periods. Shoot growth rate, mid-day stem water potential, berry size and berry composition were monitored over the season as well as final yield, cluster and pruning weights. Results showed that UTCC reduced vegetative growth as expressed by pruning weight/m while closer PD resulted in greater vegetative growth parameters. UTCC reduced vine vegetative growth to recommended values of pruning weight per m of row under both plating densities. It also reduced berry size, cluster weight and bunch rot incidence as well as increased total soluble solids and anthocyanin concentration in grapes compared to the standard herbicide treatment. The use of UTCC with supplemental irri... Presentar Todo |
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Palabras claves : |
BUNCH ROT; TANNAT; VEGETATIVE GROWTH; VITICULTURAL PRACTIVE; WATER POTENTIAL. |
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Thesagro : |
VARIEDADES; VID; VITICULTURA. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02593naa a2200301 a 4500
001 1057617
005 2017-10-02
008 2017 bl uuuu u00u1 u #d
022 $a0304-4238
024 7 $a10.1016/j.scienta.2017.09.012$2DOI
100 1 $aCONIBERTI, A.
245 $aUnder-trellis cover crop and planting density to achieve vine balance in a humid climate.$h[electronic resource]
260 $c2017
500 $aArticle history: Received 25 June 2017; Received in revised form 24 August 2017; Accepted 16 September 2017; Available online 28 September 2017.
520 $aABSTRACT. The goal of our study was to improve Tannat Vitis vinifera L. grape and wine composition, by achieving vine balance in a humid climate. We tested under-trellis cover crops (UTCC) compared to a standard floor management of alleyway cover crops and under-vine herbicide. This strategy was tested in combination with variable planting density over three growing seasons in Southern Uruguay. Two factors were evaluated in a split plot design with five replicates. Treatments were, (1) UTCC (full cover of the vineyard soil with tall fescue (Festuca arundinacea Shreb)) versus conventional alleyway tall fescue with 1.0 m wide weed-free strips under the trellis, and (2) two spacings between vines in the row (0.8 vs 1.5 m). To avoid excessive vine water stress, supplemental irrigation was used during water deficit periods. Shoot growth rate, mid-day stem water potential, berry size and berry composition were monitored over the season as well as final yield, cluster and pruning weights. Results showed that UTCC reduced vegetative growth as expressed by pruning weight/m while closer PD resulted in greater vegetative growth parameters. UTCC reduced vine vegetative growth to recommended values of pruning weight per m of row under both plating densities. It also reduced berry size, cluster weight and bunch rot incidence as well as increased total soluble solids and anthocyanin concentration in grapes compared to the standard herbicide treatment. The use of UTCC with supplemental irrigation, showed promise for achieving vine balance in high vine capacity conditions. © 2017 Elsevier B.V. All rights reserved
650 $aVARIEDADES
650 $aVID
650 $aVITICULTURA
653 $aBUNCH ROT
653 $aTANNAT
653 $aVEGETATIVE GROWTH
653 $aVITICULTURAL PRACTIVE
653 $aWATER POTENTIAL
700 1 $aFERRARI, V.
700 1 $aDISEGNA, E.
700 1 $aGARCÍA PETILLO, M.
700 1 $aLAKSO, A.N.
773 $tScientia Horticulturae, 2018$gv. 227, p. 65?74.
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