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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha : |
22/10/2014 |
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Actualizado : |
06/11/2018 |
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Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
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Autor : |
GARCÍA PETILLO, M.; GARCIA, C.; BONINO, C.; ARRIETA, I.; DELGADO, D.; CAMIO, G. |
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Afiliación : |
CLAUDIO CESAR GARCIA GALLARRETA, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
Generación de tecnología para el diseño de riego por melgas, adapada a las condiciones del Uruguay. Primer aporte. |
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Fecha de publicación : |
2014 |
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Fuente / Imprenta : |
En: Giménez, L. (ed.) Riego suplementario en cultivos y pasturas. Montevideo (UY): INIA, 2014, p. 45-58 |
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Serie : |
(Serie FPTA-INIA; 55) |
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ISSN : |
1688-924X |
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Idioma : |
Español |
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Notas : |
Proyecto FPTA 261: Respuesta física y económica al riego suplementario en cultivos y pasturas y desarrollo de la tecnología de riego por superficie en siembra directa. |
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Thesagro : |
CONSTRUCCIÓN; EFICIENCIA DEL RIEGO; PASTURAS; RIEGO POR MELGAS; RIEGO POR SUPERFICIE; SISTEMAS DE RIEGO; UNIFORMIDAD DEL RIEGO. |
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Asunto categoría : |
F06 Riego |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/8823/1/fpta-55.p.45-58.pdf
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Marc : |
LEADER 01064nam a2200277 a 4500
001 1051233
005 2018-11-06
008 2014 bl uuuu u00u1 u #d
022 $a1688-924X
100 1 $aGARCÍA PETILLO, M.
245 $aGeneración de tecnología para el diseño de riego por melgas, adapada a las condiciones del Uruguay. Primer aporte.$h[electronic resource]
260 $aEn: Giménez, L. (ed.) Riego suplementario en cultivos y pasturas. Montevideo (UY): INIA, 2014, p. 45-58$c2014
490 $a(Serie FPTA-INIA; 55)
500 $aProyecto FPTA 261: Respuesta física y económica al riego suplementario en cultivos y pasturas y desarrollo de la tecnología de riego por superficie en siembra directa.
650 $aCONSTRUCCIÓN
650 $aEFICIENCIA DEL RIEGO
650 $aPASTURAS
650 $aRIEGO POR MELGAS
650 $aRIEGO POR SUPERFICIE
650 $aSISTEMAS DE RIEGO
650 $aUNIFORMIDAD DEL RIEGO
700 1 $aGARCIA, C.
700 1 $aBONINO, C.
700 1 $aARRIETA, I.
700 1 $aDELGADO, D.
700 1 $aCAMIO, G.
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Registro original : |
INIA Treinta y Tres (TT) |
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 | Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
01/10/2014 |
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Actualizado : |
09/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
A - 1 |
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Autor : |
ALQUEZAR, B.; RODRIGO, M.J.; LADO, J.; ZACARÍAS, L. |
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Afiliación : |
JOANNA LADO LINDNER, Instituto Nacional de Investigación Agropecuaria (INIA), Uruguay. |
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Título : |
A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). |
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Fecha de publicación : |
2013 |
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Fuente / Imprenta : |
Tree Genetics & Genomes, 2013, v.9, no.5, p.1257-1269. |
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ISSN : |
1614-2942 |
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DOI : |
10.1007/s11295-013-0635-7 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013. |
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Contenido : |
ABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit.
© 2013 Springer-Verlag Berlin Heidelberg. MenosABSTRACT.
Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high... Presentar Todo |
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Palabras claves : |
ABA; CAROTENOIDS; GENE EXPRESSION; GRAPEFRUIT; LYCOPENE. |
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Thesagro : |
CAROTENO; CITRUS. |
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Asunto categoría : |
F01 Cultivo |
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Marc : |
LEADER 02702naa a2200277 a 4500
001 1050762
005 2019-10-09
008 2013 bl uuuu u00u1 u #d
022 $a1614-2942
024 7 $a10.1007/s11295-013-0635-7$2DOI
100 1 $aALQUEZAR, B.
245 $aA comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.).$h[electronic resource]
260 $c2013
500 $aArticle history: Received: 30 January 2013 / Revised: 26 March 2013 / Accepted: 15 April 2013 / Published online: 15 May 2013.
520 $aABSTRACT. Accumulation of lycopene in citrus fruits is an unusual feature restricted to selected mutants. Grapefruit (Citrus paradisi Macf.) is the Citrus specie with greater number of red-fleshed mutants, but the molecular bases of this alteration are not fully understood. To gain knowledge into the mechanisms implicated in this alteration, we conducted a comparative analysis of carotenoid profile and of the expression of genes related to carotenoid biosynthesis and catabolism in flavedo and pulp of two grapefruit cultivars with marked differences in colouration: the white Marsh and the red Star Ruby. Mature green fruit of Marsh accumulated chloroplastic carotenoids, while mature tissues lacked carotenoids. However, accumulation of downstream products such as abscisic acid (ABA) and expression of its biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (NCED1 and NCED2), increased after the onset of colouration. In contrast, red grapefruit accumulated lycopene, phytoene and phytofluene, while ABA content and NCED gene expression were lower than in Marsh, suggesting a blockage in the carotenoid biosynthetic pathway. Expression analysis of three genes of the isoprenoid pathway and nine of the carotenoid biosynthetic pathway revealed virtually no differences in flavedo and pulp between both genotypes, except for the chromoplast-specific lycopene cyclase 2 (β-LCY2) which was lower in the pulp of the red grapefruit. The proportion in the expression of the allele with high (β-LCY2a) and low (β-LCY2b) activity was also similar in the pulp of both genotypes. Therefore, results suggest that reduced expression of β-LCY2 appears to be responsible of lycopene accumulation in the red Star Ruby grapefruit. © 2013 Springer-Verlag Berlin Heidelberg.
650 $aCAROTENO
650 $aCITRUS
653 $aABA
653 $aCAROTENOIDS
653 $aGENE EXPRESSION
653 $aGRAPEFRUIT
653 $aLYCOPENE
700 1 $aRODRIGO, M.J.
700 1 $aLADO, J.
700 1 $aZACARÍAS, L.
773 $tTree Genetics & Genomes, 2013$gv.9, no.5, p.1257-1269.
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