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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
23/08/2022 |
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Actualizado : |
22/05/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Nacionales |
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Autor : |
CARRACELAS, B.; NAVAJAS, E.; VERA, B.; CIAPPESONI, G. |
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Afiliación : |
EMERITA BEATRIZ CARRACELAS MARQUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ELLY ANA NAVAJAS VALENTINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; BRENDA VERA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS GABRIEL CIAPPESONI SCARONE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
SNP arrays evaluation as tools in genetic improvement in Corriedale sheep in Uruguay. [Evaluación de paneles de SNP como herramientas en la mejora genética de ovinos Corriedale en Uruguay]. [Avaliação de painéis de SNP como ferramentas em melhoramento genético de ovinos Corriedale no Uruguai]. |
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Complemento del título : |
Animal production and pastures. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Agrociencia Uruguay, 2022, vol. 26, number 2, article e998. doi: https://doi.org/10.31285/AGRO.26.998 |
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ISSN : |
2730-5066 |
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DOI : |
10.31285/AGRO.26.998 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 09 Feb 2022; Accepted 30 May 2022; Published 19 Aug 2022. Editor: Mariana Carriquiry, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay. Correspondence: Beatriz Carracelas, bcarracelas@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (CC BY 4.0). |
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Contenido : |
ABSTRACT.- One control strategy for gastrointestinal nematodes (GIN) is genetic selection. This studys objective was to compare eggs per gram of feces (FEC) and fiber diameter (FD) estimated breeding values (EBV) and genomic EBV (GEBV) in Corriedale breed. Analysis included 19547 lambs with data, and 454, 711 and 383 genotypes from 170, 507 and 50K SNP chips, respectively. A univariate animal model was used for EBV and GEBV estimation, which included contemporary group, type of birth and dam age as fixed effects, and age at recording as covariate. Differential weights (?) were considered in the genomic relationship matrix (G), and the best fit models were identified using Akaikes Information Criterion (AIC), which were later used for GEBV and accuracies estimation. The use of only impacted on low density SNP chips. No differences were observed in mean accuracies for the whole population. However, in the genotyped subgroup accuracies increased by 2% with the 170 SNP chip (?=0.25), and by 5% (?=0.5) and 14% (?=0.75) with the 507 SNP chip. No differences were observed in FD EBV and GEBV mean accuracies. These results show that it is possible to increase GEBV accuracies despite the use of low-density chips -.-.-.-.-.-.-.-.-.-.-.-..-RESUMEN.- Una alternativa para el control de losnematodos gastrointestinales (NGI) es la selección genética. El objetivo de este trabajo fue comparar las precisiones de los valores de cría (EBV) y los EBV genómicos (GEBV) del recuento de huevos por gramo en heces (HPG) y diámetro de fibra (DF) en la raza Corriedale. El análisis incluyó 19547 corderos con datos fenotípicos y 454, 711 y 383 genotipados con paneles o chips de 170, 507 y 50K SNP, respectivamente. Los EBV y GEBV se estimaron con un modelo animal univariado que incluyó los efectos fijos:grupo contemporáneo, tipo de nacimiento y edad de la madre,y edad al registro como covariable. Se consideraron pesos diferenciales (?) en la matriz de relaciones genómicas, identificándose los modelos con mejor ajuste con el criterio de información de Akaike (AIC), que fueron utili-zados para la estimación de los GEBV y sus precisiones. El uso de ?solo impactó en el ajuste con paneles de baja densidad. No se encontraron diferencias en las precisiones promedio de la población total. En cambio, en el subgrupo de animales genotipados las precisiones aumentaron 2% con 170 SNP (?=0.25), y con 507 SNP 5% (?=0.5) y 14% (?=0.75). No hubo diferencias en precisiones de los EBV y los GEBV de DF. Los resultados muestran que es posible aumentar las precisiones de los GEBV aun con paneles de baja densidad. -.-.-.-.-.-.-.-.-.-.-.-..-RESUMO.- Uma alternativa para o controle de nematóidesgastrointestinais (NGI) é a seleção genética. O objetivo deste trabalho foi comparar as precisões dos valores genéticos estimados (EBV) e dos EBVs genômicos (GEBV) da contagem de ovos por grama de fezes (OPG) e diâmetro de fibra (DF) na raça Corriedale. Aanálise incluiu 19547 cordeiros com dados e 454, 711 e 383 genotipados de 170, 507 e 50K SNPs, respectivamente. Foram estimados os EBV e GEBV com um modelo animal univariado que incluiu efeitos fixos de grupo contemporâneo, tipo de nascimento e idade da mãe e idade no registro (covariável). Pesos diferenciais (?) foram considerados na matriz de relações genômicas, identificando os modelos com melhor ajuste via critério de informação de Akaike (AIC), os quais foram utilizados para estimar o GEBV e suas precisões. O uso de ?=0.75 somente impactou no ajuste com painéis de baixa densidade. Não foram encontradas diferenças na precisão média da população total. Em contraste, no subgrupo de animais genotipados as precisões aumentaram 2% com 170 SNPs (?=0.25), e com 507 SNPs 5% (?=0.5) e 14% (?=0.75). Não houve diferenças na precisão de EBV e GEBV de DF. Os resultados mostram que é possível aumentar a precisão de GEBVs mesmo que se utilizem painéis de baixa densidade. MenosABSTRACT.- One control strategy for gastrointestinal nematodes (GIN) is genetic selection. This studys objective was to compare eggs per gram of feces (FEC) and fiber diameter (FD) estimated breeding values (EBV) and genomic EBV (GEBV) in Corriedale breed. Analysis included 19547 lambs with data, and 454, 711 and 383 genotypes from 170, 507 and 50K SNP chips, respectively. A univariate animal model was used for EBV and GEBV estimation, which included contemporary group, type of birth and dam age as fixed effects, and age at recording as covariate. Differential weights (?) were considered in the genomic relationship matrix (G), and the best fit models were identified using Akaikes Information Criterion (AIC), which were later used for GEBV and accuracies estimation. The use of only impacted on low density SNP chips. No differences were observed in mean accuracies for the whole population. However, in the genotyped subgroup accuracies increased by 2% with the 170 SNP chip (?=0.25), and by 5% (?=0.5) and 14% (?=0.75) with the 507 SNP chip. No differences were observed in FD EBV and GEBV mean accuracies. These results show that it is possible to increase GEBV accuracies despite the use of low-density chips -.-.-.-.-.-.-.-.-.-.-.-..-RESUMEN.- Una alternativa para el control de losnematodos gastrointestinales (NGI) es la selección genética. El objetivo de este trabajo fue comparar las precisiones de los valores de cría (EBV) y los EBV genómicos (GEBV) del recuento... Presentar Todo |
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Palabras claves : |
Accuracy; FEC; GEBV; HPG; OPG; Precisão; Precisión. |
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Thesagro : |
CORRIEDALE. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16637/1/998-Article-Text-6221-1-10-20220819.pdf
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Marc : |
LEADER 05383naa a2200289 a 4500
001 1063543
005 2023-05-22
008 2022 bl uuuu u00u1 u #d
022 $a2730-5066
024 7 $a10.31285/AGRO.26.998$2DOI
100 1 $aCARRACELAS, B.
245 $aSNP arrays evaluation as tools in genetic improvement in Corriedale sheep in Uruguay. [Evaluación de paneles de SNP como herramientas en la mejora genética de ovinos Corriedale en Uruguay]. [Avaliação de painéis de SNP como ferramentas em melhoramento genético de ovinos Corriedale no Uruguai].$h[electronic resource]
260 $c2022
500 $aArticle history: Received 09 Feb 2022; Accepted 30 May 2022; Published 19 Aug 2022. Editor: Mariana Carriquiry, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay. Correspondence: Beatriz Carracelas, bcarracelas@inia.org.uy -- License: This work is licensed under a Creative Commons Attribution 4.0 International License. (CC BY 4.0).
520 $aABSTRACT.- One control strategy for gastrointestinal nematodes (GIN) is genetic selection. This studys objective was to compare eggs per gram of feces (FEC) and fiber diameter (FD) estimated breeding values (EBV) and genomic EBV (GEBV) in Corriedale breed. Analysis included 19547 lambs with data, and 454, 711 and 383 genotypes from 170, 507 and 50K SNP chips, respectively. A univariate animal model was used for EBV and GEBV estimation, which included contemporary group, type of birth and dam age as fixed effects, and age at recording as covariate. Differential weights (?) were considered in the genomic relationship matrix (G), and the best fit models were identified using Akaikes Information Criterion (AIC), which were later used for GEBV and accuracies estimation. The use of only impacted on low density SNP chips. No differences were observed in mean accuracies for the whole population. However, in the genotyped subgroup accuracies increased by 2% with the 170 SNP chip (?=0.25), and by 5% (?=0.5) and 14% (?=0.75) with the 507 SNP chip. No differences were observed in FD EBV and GEBV mean accuracies. These results show that it is possible to increase GEBV accuracies despite the use of low-density chips -.-.-.-.-.-.-.-.-.-.-.-..-RESUMEN.- Una alternativa para el control de losnematodos gastrointestinales (NGI) es la selección genética. El objetivo de este trabajo fue comparar las precisiones de los valores de cría (EBV) y los EBV genómicos (GEBV) del recuento de huevos por gramo en heces (HPG) y diámetro de fibra (DF) en la raza Corriedale. El análisis incluyó 19547 corderos con datos fenotípicos y 454, 711 y 383 genotipados con paneles o chips de 170, 507 y 50K SNP, respectivamente. Los EBV y GEBV se estimaron con un modelo animal univariado que incluyó los efectos fijos:grupo contemporáneo, tipo de nacimiento y edad de la madre,y edad al registro como covariable. Se consideraron pesos diferenciales (?) en la matriz de relaciones genómicas, identificándose los modelos con mejor ajuste con el criterio de información de Akaike (AIC), que fueron utili-zados para la estimación de los GEBV y sus precisiones. El uso de ?solo impactó en el ajuste con paneles de baja densidad. No se encontraron diferencias en las precisiones promedio de la población total. En cambio, en el subgrupo de animales genotipados las precisiones aumentaron 2% con 170 SNP (?=0.25), y con 507 SNP 5% (?=0.5) y 14% (?=0.75). No hubo diferencias en precisiones de los EBV y los GEBV de DF. Los resultados muestran que es posible aumentar las precisiones de los GEBV aun con paneles de baja densidad. -.-.-.-.-.-.-.-.-.-.-.-..-RESUMO.- Uma alternativa para o controle de nematóidesgastrointestinais (NGI) é a seleção genética. O objetivo deste trabalho foi comparar as precisões dos valores genéticos estimados (EBV) e dos EBVs genômicos (GEBV) da contagem de ovos por grama de fezes (OPG) e diâmetro de fibra (DF) na raça Corriedale. Aanálise incluiu 19547 cordeiros com dados e 454, 711 e 383 genotipados de 170, 507 e 50K SNPs, respectivamente. Foram estimados os EBV e GEBV com um modelo animal univariado que incluiu efeitos fixos de grupo contemporâneo, tipo de nascimento e idade da mãe e idade no registro (covariável). Pesos diferenciais (?) foram considerados na matriz de relações genômicas, identificando os modelos com melhor ajuste via critério de informação de Akaike (AIC), os quais foram utilizados para estimar o GEBV e suas precisões. O uso de ?=0.75 somente impactou no ajuste com painéis de baixa densidade. Não foram encontradas diferenças na precisão média da população total. Em contraste, no subgrupo de animais genotipados as precisões aumentaram 2% com 170 SNPs (?=0.25), e com 507 SNPs 5% (?=0.5) e 14% (?=0.75). Não houve diferenças na precisão de EBV e GEBV de DF. Os resultados mostram que é possível aumentar a precisão de GEBVs mesmo que se utilizem painéis de baixa densidade.
650 $aCORRIEDALE
653 $aAccuracy
653 $aFEC
653 $aGEBV
653 $aHPG
653 $aOPG
653 $aPrecisão
653 $aPrecisión
700 1 $aNAVAJAS, E.
700 1 $aVERA, B.
700 1 $aCIAPPESONI, G.
773 $tAgrociencia Uruguay, 2022, vol. 26, number 2, article e998. doi: https://doi.org/10.31285/AGRO.26.998
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
08/02/2023 |
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Actualizado : |
08/02/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CASTELLI, L.; BRANCHICCELA, B.; ZUNINO, P.; ANTÚNEZ, K. |
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Afiliación : |
LORELEY CASTELLI, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo 11600, Uruguay; MARIA BELEN BRANCHICCELA CORREA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO ZUNINO, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo 11600, Uruguay; KARINA ANTÚNEZ, Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo 11600, Uruguay. |
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Título : |
Insights into the effects of sublethal doses of pesticides glufosinate-ammonium and sulfoxaflor on honey bee health- |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Science of The Total Environment, 2023, Volume 868, article 161331. doi: http://dx.doi.org/10.1016/j.scitotenv.2022.161331 |
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ISSN : |
0048-9697 |
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DOI : |
10.1016/j.scitotenv.2022.161331 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2022; Received in revised form 27 December 2022; Accepted 29 December 2022; Available online 7 January 2023. -- Corresponding author: Antúnez, K.; Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo, Uruguay; email:kantunez03@gmail.com -- Editor: Rafael Mateo Soria. -- |
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Contenido : |
ABSTRACT.- Insect pollinators are threatened worldwide, being the exposure to multiple pesticides one of the most important stressor. The herbicide Glyphosate and the insecticide Imidacloprid are among the most used pesticides worldwide, although different studies evidenced their detrimental effects on non-target organisms. The emergence of glyphosate-resistant weeds and the recent ban of imidacloprid in Europe due to safety concerns, has prompted their replacement by new molecules, such as glufosinate-ammonium (GA) and sulfoxaflor (S). GA is a broad-spectrum and non-selective herbicide that inhibits a key enzyme in the metabolism of nitrogen, causing accumulation of lethal levels of ammonia; while sulfoxaflor is an agonist at insect nicotinic acetylcholine receptors (nAChRs) and generates excitatory responses including tremors, paralysis and mortality. Although those molecules are being increasingly used for crop protection, little is known about their effects on non-target organisms. In this study we assessed the impact of chronic and acute exposure to sublethal doses of GA and S on honey bee gut microbiota, immunity and survival. We found GA significantly reduced the number of gut bacteria, and decreased the expression of glucose oxidase, a marker of social immunity. On the other hand, S significantly increased the number of gut bacteria altering the microbiota composition, decreased the expression of lysozyme and increased the expression of hymenoptaecin. These alterations in gut microbiota and immunocompetence may lead to an increased susceptibility to pathogens. Finally, both pesticides shortened honey bee survival and increased the risk of death. Those results evidence the negative impact of GA and S on honey bees, even at single exposition to a low dose, and provide useful information to the understanding of pollinators decline. © 2022 MenosABSTRACT.- Insect pollinators are threatened worldwide, being the exposure to multiple pesticides one of the most important stressor. The herbicide Glyphosate and the insecticide Imidacloprid are among the most used pesticides worldwide, although different studies evidenced their detrimental effects on non-target organisms. The emergence of glyphosate-resistant weeds and the recent ban of imidacloprid in Europe due to safety concerns, has prompted their replacement by new molecules, such as glufosinate-ammonium (GA) and sulfoxaflor (S). GA is a broad-spectrum and non-selective herbicide that inhibits a key enzyme in the metabolism of nitrogen, causing accumulation of lethal levels of ammonia; while sulfoxaflor is an agonist at insect nicotinic acetylcholine receptors (nAChRs) and generates excitatory responses including tremors, paralysis and mortality. Although those molecules are being increasingly used for crop protection, little is known about their effects on non-target organisms. In this study we assessed the impact of chronic and acute exposure to sublethal doses of GA and S on honey bee gut microbiota, immunity and survival. We found GA significantly reduced the number of gut bacteria, and decreased the expression of glucose oxidase, a marker of social immunity. On the other hand, S significantly increased the number of gut bacteria altering the microbiota composition, decreased the expression of lysozyme and increased the expression of hymenoptaecin. These alteratio... Presentar Todo |
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Palabras claves : |
Glufosinate-ammonium; Gut microbiota; Honey bee; Immune system; Sulfoxaflor; Weed control. |
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Thesagro : |
APICULTURA; APIS MELLIFERA; MIEL. |
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Asunto categoría : |
H10 Plagas de las plantas |
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Marc : |
LEADER 03244naa a2200301 a 4500
001 1063957
005 2023-02-08
008 2023 bl uuuu u00u1 u #d
022 $a0048-9697
024 7 $a10.1016/j.scitotenv.2022.161331$2DOI
100 1 $aCASTELLI, L.
245 $aInsights into the effects of sublethal doses of pesticides glufosinate-ammonium and sulfoxaflor on honey bee health-$h[electronic resource]
260 $c2023
500 $aArticle history: Received 30 September 2022; Received in revised form 27 December 2022; Accepted 29 December 2022; Available online 7 January 2023. -- Corresponding author: Antúnez, K.; Laboratorio de Microbiología y Salud de las Abejas, Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avda. Italia 3318, Montevideo, Uruguay; email:kantunez03@gmail.com -- Editor: Rafael Mateo Soria. --
520 $aABSTRACT.- Insect pollinators are threatened worldwide, being the exposure to multiple pesticides one of the most important stressor. The herbicide Glyphosate and the insecticide Imidacloprid are among the most used pesticides worldwide, although different studies evidenced their detrimental effects on non-target organisms. The emergence of glyphosate-resistant weeds and the recent ban of imidacloprid in Europe due to safety concerns, has prompted their replacement by new molecules, such as glufosinate-ammonium (GA) and sulfoxaflor (S). GA is a broad-spectrum and non-selective herbicide that inhibits a key enzyme in the metabolism of nitrogen, causing accumulation of lethal levels of ammonia; while sulfoxaflor is an agonist at insect nicotinic acetylcholine receptors (nAChRs) and generates excitatory responses including tremors, paralysis and mortality. Although those molecules are being increasingly used for crop protection, little is known about their effects on non-target organisms. In this study we assessed the impact of chronic and acute exposure to sublethal doses of GA and S on honey bee gut microbiota, immunity and survival. We found GA significantly reduced the number of gut bacteria, and decreased the expression of glucose oxidase, a marker of social immunity. On the other hand, S significantly increased the number of gut bacteria altering the microbiota composition, decreased the expression of lysozyme and increased the expression of hymenoptaecin. These alterations in gut microbiota and immunocompetence may lead to an increased susceptibility to pathogens. Finally, both pesticides shortened honey bee survival and increased the risk of death. Those results evidence the negative impact of GA and S on honey bees, even at single exposition to a low dose, and provide useful information to the understanding of pollinators decline. © 2022
650 $aAPICULTURA
650 $aAPIS MELLIFERA
650 $aMIEL
653 $aGlufosinate-ammonium
653 $aGut microbiota
653 $aHoney bee
653 $aImmune system
653 $aSulfoxaflor
653 $aWeed control
700 1 $aBRANCHICCELA, B.
700 1 $aZUNINO, P.
700 1 $aANTÚNEZ, K.
773 $tScience of The Total Environment, 2023, Volume 868, article 161331. doi: http://dx.doi.org/10.1016/j.scitotenv.2022.161331
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